{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T04:00:33Z","timestamp":1788321633249,"version":"build-2803163510"},"reference-count":61,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,10,23]],"date-time":"2024-10-23T00:00:00Z","timestamp":1729641600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Chemokines play a crucial role in immune responses by facilitating the migration of cells expressing corresponding chemokine receptors along concentration gradients. <jats:italic>Photobacterium damselae<\/jats:italic> subsp. <jats:italic>Damselae<\/jats:italic> (PDD) and <jats:italic>Nocardia seriolae<\/jats:italic> (NS) are known to induce substantial mortality in silver pomfret populations, yet there exists a dearth of research regarding the immune response of <jats:italic>CCL<\/jats:italic>s in PDD\u2010 or NS\u2010infected silver pomfret. In our investigation, we identified 10 <jats:italic>PaCCL<\/jats:italic>s, which include one fish\u2010specific <jats:italic>CCL<\/jats:italic> (<jats:italic>PaCCL44<\/jats:italic>). Phylogenetic analysis revealed considerable diversity in <jats:italic>CCL<\/jats:italic> types and copy numbers among various teleost fishes. Notably, silver pomfret lacks specific <jats:italic>CCL<\/jats:italic> genes, with most <jats:italic>PaCCL<\/jats:italic>s exhibiting heightened expression levels in immune\u2010related organs such as the spleen and kidney, and some being expressed in mucosal immune\u2010related organs like the skin and gills. Transcriptome analysis conducted on silver pomfret infected with NS and PDD elucidated that the expression changes of <jats:italic>PaCCL<\/jats:italic>s primarily manifested in the spleen during the initial stages of NS infection, shifting to the kidney in later stages. Conversely, the expression changes of <jats:italic>PaCCL<\/jats:italic>s following PDD infection predominantly occurred in the kidney. In\u00a0vitro studies using silver pomfret spleen cell lines demonstrated an early peak in <jats:italic>PaCCL<\/jats:italic>s expression during infection, followed by gradual decline with NS treatment and rapid diminishment with PDD treatment. These findings suggest that <jats:italic>PaCCL<\/jats:italic>s primarily support the innate immunity of silver pomfret, potentially exhibiting chemotactic effects in the early infection stages, such as the synergistic action of <jats:italic>PaCCL4<\/jats:italic> and <jats:italic>PaCCL25<\/jats:italic>, and later serving as direct antibacterial agents. NS invasion is characterised by a chronic infection affecting multiple organs, whereas PDD primarily inflicts severe damage to the kidney. <jats:italic>PaCCL19a<\/jats:italic> and <jats:italic>PaCCL19b<\/jats:italic> are specific to PDD, and their expression levels may decrease in the later stages of infection due to PDD immune escape. These data offer initial insights into understanding the mechanism underlying the innate immune response of the <jats:italic>CCL<\/jats:italic> gene family in silver pomfret and provide theoretical underpinnings for fish culture practices.<\/jats:p>","DOI":"10.1111\/jfd.14032","type":"journal-article","created":{"date-parts":[[2024,10,23]],"date-time":"2024-10-23T11:09:54Z","timestamp":1729681794000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Immune Response of Silver Pomfret (<i>Pampus argenteus<\/i>) <scp>CC<\/scp> Chemokine Ligand Gene Family to <i>Photobacterium damselae<\/i> Subsp. <i>Damselae<\/i> and <i>Nocardia seriolae<\/i> Infections"],"prefix":"10.1111","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-2898-5174","authenticated-orcid":false,"given":"Kaiheng","family":"Yan","sequence":"first","affiliation":[{"name":"College of Marine Sciences Ningbo University  Ningbo China"},{"name":"Institute of Marine Science and Technology Shandong University  Qingdao China"},{"name":"Key Laboratory of Applied Marine Biotechnology Ningbo University, Ministry of Education  Ningbo China"},{"name":"Key Laboratory of Marine Biotechnology of Zhejiang Province Ningbo University  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youyi","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Marine Sciences Ningbo University  Ningbo China"},{"name":"Key Laboratory of Applied Marine Biotechnology Ningbo University, Ministry of Education  Ningbo China"},{"name":"Key Laboratory of Marine Biotechnology of Zhejiang Province Ningbo University  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanbo","family":"Li","sequence":"additional","affiliation":[{"name":"College of Marine Sciences Ningbo University  Ningbo China"},{"name":"Key Laboratory of Applied Marine Biotechnology Ningbo University, Ministry of Education  Ningbo China"},{"name":"Key Laboratory of Marine Biotechnology of Zhejiang Province Ningbo University  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengke","family":"Tang","sequence":"additional","affiliation":[{"name":"College of Marine Sciences Ningbo University  Ningbo China"},{"name":"Key Laboratory of Applied Marine Biotechnology Ningbo University, Ministry of Education  Ningbo China"},{"name":"Key Laboratory of Marine Biotechnology of Zhejiang Province Ningbo University  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yifan","family":"Xu","sequence":"additional","affiliation":[{"name":"Institute of Marine Science and Technology Shandong University  Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojun","family":"Yan","sequence":"additional","affiliation":[{"name":"College of Marine Sciences Ningbo University  Ningbo China"},{"name":"Key Laboratory of Applied Marine Biotechnology Ningbo University, Ministry of Education  Ningbo China"},{"name":"Key Laboratory of Marine Biotechnology of Zhejiang Province Ningbo University  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiabao","family":"Hu","sequence":"additional","affiliation":[{"name":"College of Marine Sciences Ningbo University  Ningbo China"},{"name":"Key Laboratory of Applied Marine Biotechnology Ningbo University, Ministry of Education  Ningbo China"},{"name":"Key Laboratory of Marine Biotechnology of Zhejiang Province Ningbo University  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yajun","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Marine Sciences Ningbo University  Ningbo China"},{"name":"Key Laboratory of Applied Marine Biotechnology Ningbo University, Ministry of Education  Ningbo China"},{"name":"Key Laboratory of Marine Biotechnology of Zhejiang Province Ningbo University  Ningbo China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,10,23]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sjbs.2021.06.026"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2011.03.011"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2015.06.001"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.179.4.2223"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.70.3.465"},{"key":"e_1_2_11_7_1","first-page":"28","article-title":"Fitting a Mixture Model by Expectation Maximization to Discover Motifs in Bipolymers","volume":"2","author":"Bailey T. 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