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The gene, protein sequences and functions of vWF in flounder <jats:styled-content style=\"fixed-case\"><jats:italic>Paralichthys olivaceus<\/jats:italic><\/jats:styled-content> (<jats:italic>Po<\/jats:italic>vWF) were analysed in this study. <jats:italic>Po<\/jats:italic>vWF possesses an 8550\u2010bp open reading frame (ORF) that encodes a 2849 amino acid protein. <jats:italic>Po<\/jats:italic>vWF mRNA is highly expressed in the heart and gill, followed by the intestine, skin, spleen, kidney, muscle and liver. <jats:italic>Po<\/jats:italic>vWF positive cells are mainly endothelial cells (ECs), predominantly located along the inner lining of blood vessels, enclosing the bloodstream. After being infected with hirame novirhabdovirus (HIRRV), flounder exhibits a dark body colour, congested fins and visceral membranes. Histopathologic analysis revealed that the ECs of diseased fish had compromised integrity, accompanied by a significant increase in number of cells within blood vessels. Immunofluorescence and ultrastructural studies showed that virions infect ECs can induce morphological and functional alterations, which lead to the release of vWF and facilitate the migration of neutrophils into tissues to exert antiviral functions. This research pinpoints the role of vWF in the immune response to HIRRV infection in teleost. It offers an in\u2010depth and all\u2010encompassing understanding of the pathophysiological interaction between HIRRV and endothelial cells during invasive infections in fish.<\/jats:p>","DOI":"10.1111\/jfd.14069","type":"journal-article","created":{"date-parts":[[2024,12,11]],"date-time":"2024-12-11T18:35:34Z","timestamp":1733942134000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Von Willebrand Factor in Weibel\u2013Palade Bodies of Endothelial Cells Involved in the Immune Response to Hirame Novirhabdovirus Infection in a Teleost <scp><i>Paralichthys olivaceus<\/i><\/scp>"],"prefix":"10.1111","volume":"48","author":[{"given":"Qiujie","family":"Gan","sequence":"first","affiliation":[{"name":"Laboratory of Pathology and Immunology of Aquatic Animals KLMME, Ocean University of China  Qingdao China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heng","family":"Chi","sequence":"additional","affiliation":[{"name":"Laboratory of Pathology and Immunology of Aquatic Animals KLMME, Ocean University of China  Qingdao China"},{"name":"Laboratory for Marine Fisheries Science and Food Production Processes Qingdao Marine Science and Technology Center  Qingdao China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xianghu","family":"Meng","sequence":"additional","affiliation":[{"name":"Laboratory of Pathology and Immunology of Aquatic Animals KLMME, Ocean University of China  Qingdao China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoqian","family":"Tang","sequence":"additional","affiliation":[{"name":"Laboratory of Pathology and Immunology of Aquatic Animals KLMME, Ocean University of China  Qingdao China"},{"name":"Laboratory for Marine Fisheries Science and Food Production Processes Qingdao Marine Science and Technology Center  Qingdao China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Xing","sequence":"additional","affiliation":[{"name":"Laboratory of Pathology and Immunology of Aquatic Animals KLMME, Ocean University of China  Qingdao China"},{"name":"Laboratory for Marine Fisheries Science and Food Production Processes Qingdao Marine Science and Technology Center  Qingdao China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiuzhen","family":"Sheng","sequence":"additional","affiliation":[{"name":"Laboratory of Pathology and Immunology of Aquatic Animals KLMME, Ocean University of China  Qingdao China"},{"name":"Laboratory for Marine Fisheries Science and Food Production Processes Qingdao Marine Science and Technology Center  Qingdao China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenbin","family":"Zhan","sequence":"additional","affiliation":[{"name":"Laboratory of Pathology and Immunology of Aquatic Animals KLMME, Ocean University of China  Qingdao China"},{"name":"Laboratory for Marine Fisheries Science and Food Production Processes Qingdao Marine Science and Technology Center  Qingdao China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,12,11]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao02285"},{"key":"e_1_2_9_3_1","first-page":"30","article-title":"Atlantic Salmon (\n                     Salmo salar\n                   L.) 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