{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:37:16Z","timestamp":1753879036223,"version":"3.41.2"},"reference-count":51,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,12,17]],"date-time":"2024-12-17T00:00:00Z","timestamp":1734393600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100007280","name":"Srinakharinwirot University","doi-asserted-by":"publisher","award":["024\/2565","307\/2566"],"award-info":[{"award-number":["024\/2565","307\/2566"]}],"id":[{"id":"10.13039\/501100007280","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2025,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The minute intestinal trematode <jats:italic>Centrocestus formosanus<\/jats:italic> is a major problem that can be found in the gills of various fish species. This parasite can destroy the gill structure of fish, leading to increased fish morbidity and mortality rates. In this study, loop\u2010mediated isothermal amplification (LAMP) based on the internal transcript spacer 1 region combined with a lateral\u2010flow dipstick (LAMP\u2010LFD) assay was used to detect <jats:styled-content style=\"fixed-case\"><jats:italic>C.\u2009formosanus<\/jats:italic><\/jats:styled-content> in goldfish, <jats:styled-content style=\"fixed-case\"><jats:italic>Carassius auratus<\/jats:italic><\/jats:styled-content>. The results showed that LAMP\u2010LFD was specific to <jats:styled-content style=\"fixed-case\"><jats:italic>C.\u2009formosanus<\/jats:italic><\/jats:styled-content> and had no cross\u2010amplification with other co\u2010infecting parasite species, close related parasite species or their hosts (both intermediate host and definitive host). The limit of detection is as low as one metacercaria per gill. In testing 40 goldfish gill samples, the LAMP\u2010LFD method showed 100% accuracy when compared to traditional morphological identification. This method can be used as a rapid diagnostic tool for <jats:styled-content style=\"fixed-case\"><jats:italic>C.\u2009formosanus<\/jats:italic><\/jats:styled-content> detection to obtain epidemiological information for monitoring, controlling, and preventing outbreaks of this parasite.<\/jats:p>","DOI":"10.1111\/jfd.14074","type":"journal-article","created":{"date-parts":[[2024,12,17]],"date-time":"2024-12-17T10:09:13Z","timestamp":1734430153000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Loop\u2010Mediated Isothermal Amplification Combined With Lateral\u2010Flow Dipstick for Detection of <i>Centrocestus formosanus<\/i> in Ornamental Fish"],"prefix":"10.1111","volume":"48","author":[{"given":"Metawee","family":"Sabaijai","sequence":"first","affiliation":[{"name":"Applied Parasitology Research Laboratory, Department of Biology, Faculty of Science Srinakharinwirot University  Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thanawan","family":"Tejangkura","sequence":"additional","affiliation":[{"name":"Applied Parasitology Research Laboratory, Department of Biology, Faculty of Science Srinakharinwirot University  Bangkok Thailand"},{"name":"Research and Innovation Unit for Diagnosis of Medical and Veterinary Important Parasites, Faculty of Science Srinakharinwirot University  Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4241-6966","authenticated-orcid":false,"given":"Thapana","family":"Chontananarth","sequence":"additional","affiliation":[{"name":"Applied Parasitology Research Laboratory, Department of Biology, Faculty of Science Srinakharinwirot University  Bangkok Thailand"},{"name":"Research and Innovation Unit for Diagnosis of Medical and Veterinary Important Parasites, Faculty of Science Srinakharinwirot University  Bangkok Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,12,17]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_11_2_1","DOI":"10.4172\/21559546.1000108"},{"volume-title":"AVMA Guidelines for the Euthanasia of Animals: 2020 Edition","year":"2020","author":"American Veterinary Medical Association","key":"e_1_2_11_3_1"},{"doi-asserted-by":"publisher","key":"e_1_2_11_4_1","DOI":"10.1155\/2022\/4928009"},{"doi-asserted-by":"publisher","key":"e_1_2_11_5_1","DOI":"10.1016\/0166\u20106851(92)90109\u2010w"},{"doi-asserted-by":"publisher","key":"e_1_2_11_6_1","DOI":"10.1645\/12\u201037.1"},{"doi-asserted-by":"publisher","key":"e_1_2_11_7_1","DOI":"10.2307\/3272966"},{"doi-asserted-by":"publisher","key":"e_1_2_11_8_1","DOI":"10.1016\/j.apjtb.2016.11.018"},{"key":"e_1_2_11_9_1","first-page":"1016","article-title":"Trematode Infections of the Freshwater Snail Family Thiaridae in the Khek River, Thailand","volume":"38","author":"Dechruksa W.","year":"2007","journal-title":"Southeast Asian Journal of Tropical Medicine and Public Health"},{"doi-asserted-by":"publisher","key":"e_1_2_11_10_1","DOI":"10.1590\/1519\u20106984.15015"},{"doi-asserted-by":"publisher","key":"e_1_2_11_11_1","DOI":"10.20546\/ijcmas.2018.707.112"},{"doi-asserted-by":"publisher","key":"e_1_2_11_12_1","DOI":"10.2478\/s11687\u2010007\u20100034\u20104"},{"doi-asserted-by":"publisher","key":"e_1_2_11_13_1","DOI":"10.3347\/kjp.2008.46.1.49"},{"doi-asserted-by":"publisher","key":"e_1_2_11_14_1","DOI":"10.1016\/j.aquaculture.2021.737660"},{"doi-asserted-by":"publisher","key":"e_1_2_11_15_1","DOI":"10.1007\/s10499\u2010015\u20109909\u20104"},{"doi-asserted-by":"publisher","key":"e_1_2_11_16_1","DOI":"10.1016\/j.actatropica.2016.01.007"},{"key":"e_1_2_11_17_1","first-page":"7","article-title":"Prevalence and Morphological Changes on Fish Gills of Cyprinoid Fish Infected With Centrocestus formosanus Metacercariae From Li River, Lamphun Province","volume":"32","author":"Kuntatum R.","year":"2019","journal-title":"Microscopy and Microanalysis Research"},{"doi-asserted-by":"publisher","key":"e_1_2_11_18_1","DOI":"10.1093\/bjaceaccp\/mkn041"},{"doi-asserted-by":"publisher","key":"e_1_2_11_19_1","DOI":"10.1016\/j.fm.2017.10.001"},{"volume-title":"AFS\u2010FHS (American Fisheries Society\u2010Fish Health Section). 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