{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T08:32:53Z","timestamp":1775809973332,"version":"3.50.1"},"reference-count":21,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,1,7]],"date-time":"2025-01-07T00:00:00Z","timestamp":1736208000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100009649","name":"University of the West Indies","doi-asserted-by":"publisher","award":["Officeofthecampusregistrar,schoolofgraduatestudiesandresearch"],"award-info":[{"award-number":["Officeofthecampusregistrar,schoolofgraduatestudiesandresearch"]}],"id":[{"id":"10.13039\/100009649","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009649","name":"University of the West Indies","doi-asserted-by":"publisher","award":["CRP.5.OCT18.81"],"award-info":[{"award-number":["CRP.5.OCT18.81"]}],"id":[{"id":"10.13039\/100009649","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Potentially zoonotic Mycobacterium spp. are impacting freshwater ornamental fish in Trinidad and Tobago. Clinical cases presented at the Aquatic Animal Health Unit of The University of the West Indies, School of Veterinary Medicine, from September 2011 to September 2018 indicated the presence of piscine mycobacteriosis in freshwater ornamental fish from locations throughout Trinidad and Tobago. Subsequently, an investigation was conducted from June 2018 to December 2021 to identify the specific Mycobacterium spp. involved. Nested polymerase chain reaction (PCR) was used to amplify and later sequence the 723 bp of the rpoB. Analysis of region V of the rpoB offers similar discriminatory power as concatenation or whole genome analysis of Mycobacterium spp., and serves as an acceptable method for differentiating and discriminating between species in this genus. With respect to tank\u2010reared freshwater ornamental fish, this study identified <jats:italic>M. fortuitum<\/jats:italic> (98.8% identity), <jats:italic>M. liflandii<\/jats:italic> (100% identity), <jats:italic>M. stomatepiae<\/jats:italic> (97.2% identity), <jats:italic>M. pseudoshottsii<\/jats:italic> related (96.9% identity), two <jats:italic>M. stomatepiae<\/jats:italic> related (95.4% and 96.7% identity), <jats:italic>M. immunogenum<\/jats:italic> related (93.4% identity), and 17 <jats:italic>M. insubricum<\/jats:italic> related (91.4%\u201395.2% identity). Additionally, seven mycobacteria related to <jats:italic>M. insubricum<\/jats:italic> were identified in wild\u2010caught guppies. These findings suggest that both known, and potentially novel Mycobacterium spp. are circulating, and adversely impacting the local populations of freshwater ornamental fish in Trinidad and Tobago.<\/jats:p>","DOI":"10.1111\/jfd.14079","type":"journal-article","created":{"date-parts":[[2025,1,7]],"date-time":"2025-01-07T12:03:31Z","timestamp":1736251411000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Identification of Potentially Novel <i>Mycobacterium<\/i> Spp. In Freshwater Ornamental Fish in Trinidad and Tobago"],"prefix":"10.1111","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3036-015X","authenticated-orcid":false,"given":"Lemar","family":"Blake","sequence":"first","affiliation":[{"name":"Department of Basic Veterinary Sciences, School of Veterinary Medicine, Faculty of Medical Sciences The University of the West Indies  St. Augustine W.I Trinidad and Tobago"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arianne Brown","family":"Jordan","sequence":"additional","affiliation":[{"name":"Department of Para\u2010Clinical Sciences, Biochemistry Unit, Faculty of Medical Sciences The University of the West Indies  St. Augustine W.I Trinidad and Tobago"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soren","family":"Nicholls","sequence":"additional","affiliation":[{"name":"Department of Para\u2010Clinical Sciences, Biochemistry Unit, Faculty of Medical Sciences The University of the West Indies  St. Augustine W.I Trinidad and Tobago"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6054-9634","authenticated-orcid":false,"given":"Esteban","family":"Soto","sequence":"additional","affiliation":[{"name":"Department of Medicine and Epidemiology, School of Veterinary Medicine University of California\u2010Davis  Davis California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luke","family":"Iwanowicz","sequence":"additional","affiliation":[{"name":"U.S. Department of Agriculture\/Agricultural Research Service National Center for Cool and Cold Water Aquaculture  Leetown West Virginia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rod","family":"Suepaul","sequence":"additional","affiliation":[{"name":"Department of Basic Veterinary Sciences, School of Veterinary Medicine, Faculty of Medical Sciences The University of the West Indies  St. Augustine W.I Trinidad and Tobago"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher","family":"Oura","sequence":"additional","affiliation":[{"name":"Department of Basic Veterinary Sciences, School of Veterinary Medicine, Faculty of Medical Sciences The University of the West Indies  St. Augustine W.I Trinidad and Tobago"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayanna Carla N.","family":"Phillips Savage","sequence":"additional","affiliation":[{"name":"Department of Clinical Veterinary Sciences, School of Veterinary Medicine, Faculty of Medical Sciences The University of the West Indies  St. Augustine W.I Trinidad and Tobago"},{"name":"Department of Population Health Sciences, Virginia\u2010Maryland College of Veterinary Medicine Virginia Polytechnic Institute and State University  Blacksburg Virginia USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,1,7]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.12.5699-5708.2003"},{"issue":"10","key":"e_1_2_7_3_1","first-page":"182","article-title":"Comparison of Conventional and Molecular Methods Used for Diagnosis of \n                     mycobacterium tuberculosis\n                   in Clinical Samples","volume":"65","author":"Akkaya O.","year":"2019","journal-title":"Clinical Laboratory"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-56604-7"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1099\/acmi.0.000245"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.63222-0"},{"key":"e_1_2_7_7_1","volume-title":"Mycobacterial Infections of Fish","author":"Francis\u2010Floyd R.","year":"2011"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2008.05.012"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.00714"},{"key":"e_1_2_7_10_1","volume-title":"Clinical Microbiology Procedures Handbook","author":"Isenberg H. 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