{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T15:22:32Z","timestamp":1767972152173,"version":"3.49.0"},"reference-count":32,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,2,13]],"date-time":"2025-02-13T00:00:00Z","timestamp":1739404800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2024A1515012674"],"award-info":[{"award-number":["2024A1515012674"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021171","name":"Basic and Applied Basic Research Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2024A1515010074"],"award-info":[{"award-number":["2024A1515010074"]}],"id":[{"id":"10.13039\/501100021171","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2025,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\"><jats:italic>Nocardia seriolae<\/jats:italic><\/jats:styled-content> is the main pathogen causing fish nocardiosis, which can infect various aquatic freshwater and marine fish species. We have previously confirmed that the heme\u2010binding protein of <jats:styled-content style=\"fixed-case\"><jats:italic>N.\u2009seriolae<\/jats:italic><\/jats:styled-content> (NsHBP) is a secreted protein relating to bacterial virulence. In the present study, a \u0394NsHBP mutant of <jats:styled-content style=\"fixed-case\"><jats:italic>N.\u2009seriolae<\/jats:italic><\/jats:styled-content> was successfully generated by deleting <jats:italic>NsHBP<\/jats:italic> gene, and it was further used for attenuated vaccine development and evaluation in hybrid snakehead (<jats:styled-content style=\"fixed-case\"><jats:italic>Channa maculata<\/jats:italic><\/jats:styled-content> \u2640\u2009\u00d7\u2009<jats:styled-content style=\"fixed-case\"><jats:italic>Channa argus<\/jats:italic><\/jats:styled-content> \u2642). The LD<jats:sub>50<\/jats:sub> of strain \u0394NsHBP by intraperitoneal injection in hybrid snakehead was presented at 5.47\u2009\u00d7\u200910<jats:sup>7<\/jats:sup>\u2009CFU\/mL, significantly (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.01) higher than that of the wild\u2010type strain at 5.28\u2009\u00d7\u200910<jats:sup>5<\/jats:sup>\u2009CFU\/mL, indicating the virulent reduction of strain \u0394NsHBP in comparison to wild\u2010type strain. The live attenuated vaccine used strain \u0394NsHBP at sub\u2010clinical dosages (LD<jats:sub>15<\/jats:sub>, 1.26\u2009\u00d7\u200910<jats:sup>7<\/jats:sup>\u2009CFU\/mL) to immunise the fish by intraperitoneal injection for 56\u2009days could provide a relative percentage survival (RPS) at 82.40% against artificial challenge with wild\u2010type strain ZJ0503. Additionally, serum enzymes activities of lysozyme (LZM), peroxidase (POD), acid phosphatase (ACP), alkaline phosphatase (AKP), superoxide dismutase (SOD) as well as specific antibody (IgM) titers were induced in fish following \u0394NsHBP vaccination. Furthermore, the expression of immune\u2010related genes (<jats:italic>TNF\u03b1<\/jats:italic>, <jats:italic>IL\u20101\u03b2<\/jats:italic>, <jats:italic>MHCI\u03b1<\/jats:italic>, <jats:italic>MHCII\u03b1<\/jats:italic>, <jats:italic>CD4<\/jats:italic> and <jats:italic>CD8\u03b1<\/jats:italic>) was significantly increased in comparison to the control group during immunisation period with \u0394NsHBP in fish. Taken together, it is revealed that the deleted strain \u0394NsHBP could be a potential candidate for live attenuated vaccine development to control fish nocardiosis in aquaculture.<\/jats:p>","DOI":"10.1111\/jfd.14090","type":"journal-article","created":{"date-parts":[[2025,2,13]],"date-time":"2025-02-13T05:00:38Z","timestamp":1739422838000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Construction of Heme\u2010Binding Protein Deleted Strain and Using It as an Attenuated Vaccine Against <i>Nocardia seriolae<\/i> in Hybrid Snakehead (<i>Channa maculata<\/i> \u2640\u2009\u00d7\u2009<i>Channa argus<\/i> \u2642)"],"prefix":"10.1111","volume":"48","author":[{"given":"Yanqi","family":"Wu","sequence":"first","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture Shenzhen Institute of Guangdong Ocean University, Fisheries College of Guangdong Ocean University  Zhanjiang Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yansheng","family":"Liu","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture Shenzhen Institute of Guangdong Ocean University, Fisheries College of Guangdong Ocean University  Zhanjiang Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huiyuan","family":"Yang","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture Shenzhen Institute of Guangdong Ocean University, Fisheries College of Guangdong Ocean University  Zhanjiang Guangdong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wanna","family":"Sirimanapong","sequence":"additional","affiliation":[{"name":"Veterinary Aquatic Animal Research &amp; Health Care Unit, Department of Clinical Sciences and Public Health, Faculty of 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