{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T00:52:19Z","timestamp":1791420739560,"version":"4.3.3"},"reference-count":52,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,2,27]],"date-time":"2025-02-27T00:00:00Z","timestamp":1740614400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100017170","name":"Thailand Science Research and Innovation","doi-asserted-by":"publisher","award":["FFB670076\/0337"],"award-info":[{"award-number":["FFB670076\/0337"]}],"id":[{"id":"10.13039\/501100017170","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2025,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Infectious spleen and kidney necrosis virus (ISKNV) has a broad host range and poses a significant threat to aquaculture species. Herein, we report two disease outbreaks in snakeskin gourami (<jats:styled-content style=\"fixed-case\"><jats:italic>Trichopodus pectoralis<\/jats:italic><\/jats:styled-content>) in Thailand, marked by skin haemorrhage, scale loss, internal organ discoloration and degeneration and a mortality exceeding 60%. Additionally, three spot gourami (<jats:styled-content style=\"fixed-case\"><jats:italic>Trichopodus trichopterus<\/jats:italic><\/jats:styled-content>) cultured in the same ponds along with snakeskin gourami were found to be affected and tested positive for the virus. Histopathological examination revealed extensive necrosis of haematopoietic tissues in the kidneys and spleen, accompanied by the presence of numerous megalocytic cells in both fish species. Real\u2010time PCR assays, using protocols targeting the major capsid protein (MCP) gene in a broad range of <jats:italic>Megalocytivirus<\/jats:italic> genotypes, confirmed the presence of ISKNV in the infected fish. Furthermore, molecular and phylogenetic analyses based on the MCP gene (1362\u2009bp) and the adenosine triphosphatase (ATPase) gene (720\u2009bp) revealed that the ISKNV strains in gourami and other fish hosts were closely related, suggesting possible cross\u2010species transmission. This report extends the host range of ISKNV and highlights the need to prevent the spread of this virus across species boundaries. Further research is needed to understand the pathogenicity and transmission routes of the virus, gain insights into its epidemiology and develop strategies to mitigate disease outbreaks.<\/jats:p>","DOI":"10.1111\/jfd.14110","type":"journal-article","created":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T01:17:33Z","timestamp":1741742253000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Natural Infection of Two Gourami Species (<i>Trichopodus<\/i> spp.) With Infectious Spleen and Kidney Necrosis Virus (<scp>ISKNV<\/scp>): Clinical, Molecular and Histopathological Findings"],"prefix":"10.1111","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5318-6265","authenticated-orcid":false,"given":"Nguyen","family":"Dinh\u2010Hung","sequence":"first","affiliation":[{"name":"Aquaculture Pathology Laboratory, School of Animal &amp; Comparative Biomedical Sciences The University of Arizona  Tucson Arizona USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ha Thanh","family":"Dong","sequence":"additional","affiliation":[{"name":"Aquaculture and Aquatic Resources Management (AARM), School of Environment, Resources and Development Asian Institute of Technology (AIT)  Pathum Thani Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kornsunee","family":"Phiwsaiya","sequence":"additional","affiliation":[{"name":"Fish Health Platform, Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Faculty of Science Mahidol University  Bangkok Thailand"},{"name":"National Center for Genetic Engineering and Biotechnology (BIOTEC) National Science and Technology Development Agency (NSTDA)  Pathum Thani Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suwimon","family":"Taengphu","sequence":"additional","affiliation":[{"name":"Fish Health Platform, Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Faculty of Science Mahidol University  Bangkok Thailand"},{"name":"National Center for Genetic Engineering and Biotechnology (BIOTEC) National Science and Technology Development Agency (NSTDA)  Pathum Thani Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nguyen Vu","family":"Linh","sequence":"additional","affiliation":[{"name":"Department of Animal and Aquatic Sciences, Faculty of Agriculture Chiang Mai University  Chiang Mai Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2216-3700","authenticated-orcid":false,"given":"Satid","family":"Chatchaiphan","sequence":"additional","affiliation":[{"name":"Department of Aquaculture, Faculty of Fisheries Kasetsart University  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5711-9294","authenticated-orcid":false,"given":"Channarong","family":"Rodkhum","sequence":"additional","affiliation":[{"name":"Center of Excellence in Fish Infectious Diseases (CE FID), Department of Veterinary Microbiology, Faculty of Veterinary Science Chulalongkorn University  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hung N.","family":"Mai","sequence":"additional","affiliation":[{"name":"Aquaculture Pathology Laboratory, School of Animal &amp; Comparative Biomedical Sciences The University of Arizona  Tucson Arizona USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arun K.","family":"Dhar","sequence":"additional","affiliation":[{"name":"Aquaculture Pathology Laboratory, School of Animal &amp; Comparative Biomedical Sciences The University of Arizona  Tucson Arizona USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5599-4343","authenticated-orcid":false,"given":"Saengchan","family":"Senapin","sequence":"additional","affiliation":[{"name":"Fish Health Platform, Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Faculty of Science Mahidol University  Bangkok Thailand"},{"name":"National Center for Genetic Engineering and Biotechnology (BIOTEC) National Science and Technology Development Agency (NSTDA)  Pathum Thani Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,2,27]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2023.740330"},{"key":"e_1_2_9_3_1","first-page":"11","article-title":"Distribution and Detection of Megalocytivirus in Ornamental Fish in Thailand","volume":"43","author":"Baoprasertkul P.","year":"2019","journal-title":"Journal of Fisheries and Environment"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.12822"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1099\/jgv.0.000818"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-540-68618-7_4"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005074"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2023.739583"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2022.847977"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2022.739173"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2017.06.054"},{"key":"e_1_2_9_12_1","unstructured":"FAO.2024.\u201cThailand. 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