{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T02:36:51Z","timestamp":1784169411436,"version":"3.55.0"},"reference-count":34,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,3,1]],"date-time":"2025-03-01T00:00:00Z","timestamp":1740787200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100006370","name":"Small Business Innovation Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006370","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100028802","name":"Society of Transnational Academic Researchers Scholars Network","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100028802","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Piscine francisellosis is a highly infectious and economically significant disease caused by <jats:italic>Francisella orientalis<\/jats:italic> in tilapia (<jats:italic>Oreochromis<\/jats:italic> spp.). There are currently no approved treatments or commercial vaccines for this disease in cultured fish. Injectable vaccines using diatoms as antigen expression vectors have demonstrated efficacy in tilapia models; however, no oral vaccine trials have been performed. We hypothesised that fusion proteins consisting of <jats:styled-content style=\"fixed-case\"><jats:italic>F. orientalis<\/jats:italic><\/jats:styled-content> IglC and flagellin expressed in <jats:styled-content style=\"fixed-case\"><jats:italic>Thalassiosira pseudonana<\/jats:italic><\/jats:styled-content> diatoms will act as self\u2010adjuvanting antigen delivery systems to confer a protective immune response against <jats:styled-content style=\"fixed-case\"><jats:italic>F. orientalis<\/jats:italic><\/jats:styled-content> in tilapia when administered as top\u2010coated feed. Different treatments were immunised and subsequently provided with one or two boosters prior to challenge. Fish were challenged with virulent <jats:styled-content style=\"fixed-case\"><jats:italic>F. orientalis<\/jats:italic><\/jats:styled-content> via immersion thirty days post initial immunisation. Tilapia immune response was assessed 24\u2009h post\u2010challenge by quantifying <jats:italic>il\u201012, il\u201010, ifn\u2010\u03b3<\/jats:italic> and <jats:italic>tgf\u2010\u03b2<\/jats:italic> gene expression in gills and internal organs. Morbidity and mortality were monitored for 21\u2009days after challenge and bacterial load was assessed in survivors. Findings indicate significant changes in the expression of <jats:italic>ifn\u2010\u03b3<\/jats:italic> and <jats:italic>tgf\u2010\u03b2<\/jats:italic> in immunised fish, but similar mortality rates and bacterial load across all exposed groups.<\/jats:p>","DOI":"10.1111\/jfd.14111","type":"journal-article","created":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T09:57:51Z","timestamp":1741687071000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Immune Response of Nile Tilapia (<scp><i>Oreochromis niloticus<\/i><\/scp>) Vaccinated With Diatom\u2010Based Oral Vaccines Against Piscine Francisellosis"],"prefix":"10.1111","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-2190-9042","authenticated-orcid":false,"given":"Collin","family":"Meyer","sequence":"first","affiliation":[{"name":"Department of Medicine and Epidemiology School of Veterinary Medicine, University of California  Davis California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roshan P.","family":"Shrestha","sequence":"additional","affiliation":[{"name":"PhycoVax LLC  San Diego California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruth","family":"Milston\u2010Clements","sequence":"additional","affiliation":[{"name":"Department of Microbiology, College of Science Oregon State University  Corvallis Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Gibson","sequence":"additional","affiliation":[{"name":"Department of Microbiology, College of Science Oregon State University  Corvallis Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taylor I.","family":"Heckman","sequence":"additional","affiliation":[{"name":"Department of Medicine and Epidemiology School of Veterinary Medicine, University of California  Davis California USA"},{"name":"Department of Wildlife, Fisheries and Aquaculture College of Forest Resources, Mississippi State University  Starkville Mississippi USA"},{"name":"Thad Cochran National Warmwater Aquaculture Center, Mississippi Agriculture and Forestry Experiment Station Mississippi State University  Stoneville Mississippi USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeinab","family":"Yazdi","sequence":"additional","affiliation":[{"name":"Department of Medicine and Epidemiology School of Veterinary Medicine, University of California  Davis California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6054-9634","authenticated-orcid":false,"given":"Esteban","family":"Soto","sequence":"additional","affiliation":[{"name":"Department of Medicine and Epidemiology School of Veterinary Medicine, University of California  Davis California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms8091396"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodres.2015.05.021"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02172403"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102761.2010.01226.x"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.01099"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0007114512005223"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253\u2010017\u20108267\u20108"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/vaccines8020283"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574\u20106941.2010.00973.x"},{"key":"e_1_2_10_11_1","volume-title":"The State of World Fisheries and Aquaculture 2024\u2014Blue Transformation in Action","author":"Food and Agriculture Organization of the United Nations","year":"2024"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2021.03.019"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00688\u201013"},{"key":"e_1_2_10_14_1","volume-title":"Technical Note 170 GFP Quantification With DeNovix DS\u201011 Series","author":"Jones A.","year":"2020"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2020.735022"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1080\/13657305.2015.994240"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao053249"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2015.00519"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.1.292"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2015.00354"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/raq.12737"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1900137"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2016.03.003"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1529\u20108817.2006.00269.x"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2020.07.045"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/jam.13918"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.1128\/ec.00209\u201014"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1177\/1040638711407058"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao02204"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102761.2009.01070.x"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2014.00060"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfb.12004"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.03054"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2013.06.013"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/jb\/mvq043"}],"container-title":["Journal of Fish Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.14111","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.14111","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,8,11]],"date-time":"2025-08-11T09:24:14Z","timestamp":1754904254000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jfd.14111"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3]]},"references-count":34,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2025,7]]}},"alternative-id":["10.1111\/jfd.14111"],"URL":"https:\/\/doi.org\/10.1111\/jfd.14111","archive":["Portico"],"relation":{},"ISSN":["0140-7775","1365-2761"],"issn-type":[{"value":"0140-7775","type":"print"},{"value":"1365-2761","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3]]},"assertion":[{"value":"2024-11-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-02-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e14111"}}