{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T14:43:45Z","timestamp":1758120225441,"version":"3.43.0"},"reference-count":25,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,3,19]],"date-time":"2025-03-19T00:00:00Z","timestamp":1742342400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>A 10\u2010month\u2010old intact male blue Siamese fighting fish (<jats:styled-content style=\"fixed-case\"><jats:italic>Betta splendens<\/jats:italic><\/jats:styled-content>) was presented to the Purdue University Small Animal Hospital with multiple bilateral masses on the caudal flank. Comprehensive evaluation using impression cytology, histopathology and transmission electron microscopy (TEM) revealed a tumour composed of a mixed\u2010cell population of iridophores and melanophores, consistent with a mixed\u2010cell irido\u2010melanophoroma. The tumour exhibited clinical and cytohistological characteristics of malignancy, including rapid enlargement, cellular and nuclear atypia, tissue invasion and tumour emboli formation. Interestingly, mild mononuclear inflammation was observed, and mycobacterial culture isolated an acid\u2010fast\u2010positive bacterium identified as <jats:styled-content style=\"fixed-case\"><jats:italic>Mycobacterium chelonae<\/jats:italic><\/jats:styled-content> through Sanger sequencing of <jats:italic>rpoB<\/jats:italic> and <jats:italic>16S rRNA<\/jats:italic>, combined with Basic Local Alignment Search Tool analysis. This is the first case report of a malignant piscine chromatophoroma with concurrent inflammation and isolation of <jats:italic>Mycobacterium<\/jats:italic>, highlighting the need for further studies to investigate a potential association.<\/jats:p>","DOI":"10.1111\/jfd.14115","type":"journal-article","created":{"date-parts":[[2025,3,19]],"date-time":"2025-03-19T10:11:08Z","timestamp":1742379068000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Malignant Mixed Irido\u2010Melanophoroma in a <i>Betta splendens<\/i> with Concurrent <i>Mycobacterium chelonae<\/i> Isolation\u2014A Case Report"],"prefix":"10.1111","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-9765-6076","authenticated-orcid":false,"given":"Zi An","family":"Yu","sequence":"first","affiliation":[{"name":"Department of Comparative Pathobiology Purdue University College of Veterinary Medicine  West Lafayette Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lorraine A.","family":"Corriveau","sequence":"additional","affiliation":[{"name":"Purdue University Veterinary Teaching Hospital Purdue University College of Veterinary Medicine  West Lafayette Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joanne B.","family":"Messick","sequence":"additional","affiliation":[{"name":"Department of Comparative Pathobiology Purdue University College of Veterinary Medicine  West Lafayette Indiana USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,3,19]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micron.2011.07.002"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2024.109523"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1136\/vetreccr\u20102019\u2010000840"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2023.740191"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2023.739822"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2018.06.005"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpc.2011.03.007"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.2108\/zsj.12.811"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1086\/BBLv224n1p14"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-981-16-1490-3"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.1989.76"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1901021116"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432\u20101033.1990.tb15403.x"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/aff2.74"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmor.1051600107"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rvsc.2010.09.010"},{"issue":"8","key":"e_1_2_7_18_1","first-page":"1761","article-title":"Chromatophoromas and Chromatophore Hyperplasia in Pacific Rockfish","volume":"53","author":"Okihiro M. 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