{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T07:01:17Z","timestamp":1792047677084,"version":"4.4.0"},"reference-count":30,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T00:00:00Z","timestamp":1745366400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Betanodaviruses, members of the <jats:italic>Nodaviridae<\/jats:italic> family, are the causative agents of viral nervous necrosis in fish, resulting in great economic losses worldwide. This is the first case study describing the detection and confirmation of viral nervous necrosis in pot\u2010bellied seahorses (<jats:styled-content style=\"fixed-case\"><jats:italic>Hippocampus abdominalis<\/jats:italic><\/jats:styled-content>) in Australia. Clinical signs of this infection in the seahorses included whirling, floating and cessation of feeding. Histopathological findings included prominent vacuolation in the grey matter of the brain and in the retina of the eye. Betanodavirus infection was initially detected by betanodavirus reverse transcriptase quantitative PCR and nested PCR. Sequencing confirmed the red\u2010spotted grouper nervous necrosis virus (RGNNV) genotype of betanodavirus. The RGNNV genotype of betanodavirus has previously been detected in Australia in fish, including barramundi (<jats:styled-content style=\"fixed-case\"><jats:italic>Lates calcarifer<\/jats:italic><\/jats:styled-content>) and striped trumpeter (<jats:styled-content style=\"fixed-case\"><jats:italic>Latris lineata<\/jats:italic><\/jats:styled-content>). Immunohistochemistry using antibodies directed against the capsid protein of betanodavirus demonstrated intralesional virus antigen. Betanodavirus targeted the brain, spinal cord and retina, causing intracytoplasmic vacuolation of neuronal processes and neuronal necrosis.<\/jats:p>","DOI":"10.1111\/jfd.14131","type":"journal-article","created":{"date-parts":[[2025,4,23]],"date-time":"2025-04-23T12:24:50Z","timestamp":1745411090000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Viral Nervous Necrosis due to Betanodavirus: A Case Study in Pot\u2010Bellied Seahorses (<i>Hippocampus abdominals<\/i>)"],"prefix":"10.1111","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-3408-5745","authenticated-orcid":false,"given":"Stephen M.","family":"Pefanis","sequence":"first","affiliation":[{"name":"Department of Natural Resources and Environment Animal Health Laboratory (AHL)  Launceston Tasmania Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Graeme","family":"Knowles","sequence":"additional","affiliation":[{"name":"Department of Natural Resources and Environment Animal Health Laboratory (AHL)  Launceston Tasmania Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2402-3843","authenticated-orcid":false,"given":"Peter G.","family":"Mohr","sequence":"additional","affiliation":[{"name":"CSIRO Australian Centre for Disease Preparedness (ACDP)  East Geelong Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kate","family":"Swift","sequence":"additional","affiliation":[{"name":"Department of Natural Resources and Environment Animal Health Laboratory (AHL)  Launceston Tasmania Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jemma","family":"Bergfeld","sequence":"additional","affiliation":[{"name":"CSIRO Australian Centre for Disease Preparedness (ACDP)  East Geelong Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-3982-6465","authenticated-orcid":false,"given":"Teresa K.","family":"Wilson","sequence":"additional","affiliation":[{"name":"Department of Natural Resources and Environment Animal Health Laboratory (AHL)  Launceston Tasmania Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marianne","family":"Douglas","sequence":"additional","affiliation":[{"name":"Department of Natural Resources and Environment Animal Health Laboratory (AHL)  Launceston Tasmania Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachelle","family":"Hawkins","sequence":"additional","affiliation":[{"name":"Department of Natural Resources and Environment Animal Health Laboratory (AHL)  Launceston Tasmania Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Hoad","sequence":"additional","affiliation":[{"name":"CSIRO Australian Centre for Disease Preparedness (ACDP)  East Geelong Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam","family":"Costin","sequence":"additional","affiliation":[{"name":"CSIRO Australian Centre for Disease Preparedness (ACDP)  East Geelong Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4934-7369","authenticated-orcid":false,"given":"Jacqueline","family":"Morris","sequence":"additional","affiliation":[{"name":"CSIRO Australian Centre for Disease Preparedness (ACDP)  East Geelong Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Cummins","sequence":"additional","affiliation":[{"name":"CSIRO Australian Centre for Disease Preparedness (ACDP)  East Geelong Victoria Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas J. 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