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A new strain of an amoebozoan, <jats:italic>Ptolemeba bulliensis<\/jats:italic> Watson et\u00a0al. 2014 (Tubulinea) was isolated, studied and identified from the gills of farmed trout showing signs of NGD in the northwest of Russia. The strain was identified using morphological and molecular (small subunit [SSU] ribosomal RNA gene sequence data) characters. Actin and cytochrome c oxidase (Cox1) gene sequences were obtained for the representative of <jats:italic>Ptolemeba<\/jats:italic>\u00a0for the first time. A peculiar feature of the Cox1 gene is the presence of group I intron that contains a 177 amino acids long open reading frame encoding a homing endonuclease. This is the first case of a group I intron in the Cox1 gene in Amoebozoa apart from <jats:italic>Dictyostelium<\/jats:italic>. In the experimental study, the studied amoebae demonstrate a broad range of salinity tolerance. They are capable of survival and reproduction in a range of salinities between freshwater Prescott and James medium and 18\u2030, but could not survive or reproduce in 25\u2030 and above. This indicates a possibility of this species' distribution through brackish water biotopes and shows that in case of pathogenicity for the farmed fish, the methods of treatment based on increasing salinity may be inefficient. In spite of the lack of direct evidence for pathogenicity, repeated observations of <jats:italic>P<\/jats:italic>. <jats:italic>bulliensis<\/jats:italic> isolated from the gills may suggest its association with the disease.<\/jats:p>","DOI":"10.1111\/jfd.14132","type":"journal-article","created":{"date-parts":[[2025,4,10]],"date-time":"2025-04-10T05:36:24Z","timestamp":1744263384000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["<i>Ptolemeba bulliensis<\/i> Watson et\u00a0al. 2014 (Amoebozoa, Tubulinea) From Freshwater NGD\u2010Affected Rainbow Trout (<i>Oncorhynchus mykiss<\/i> Walbaum, 1792) Gills Tolerates Brackish Water Conditions"],"prefix":"10.1111","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3818-3610","authenticated-orcid":false,"given":"Alexander","family":"Kudryavtsev","sequence":"first","affiliation":[{"name":"Laboratory of Cellular and Molecular Protistology Zoological Institute of the Russian Academy of Sciences  Saint\u2010Petersburg Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ekaterina","family":"Volkova","sequence":"additional","affiliation":[{"name":"Laboratory of Cellular and Molecular Protistology Zoological Institute of the Russian Academy of Sciences  Saint\u2010Petersburg Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9917-186X","authenticated-orcid":false,"given":"Aleksey","family":"Parshukov","sequence":"additional","affiliation":[{"name":"Institute of Biology of the Karelian Research Centre RAS  Petrozavodsk Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,4,10]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/jeu.12691"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022\u20102836(05)80360\u20102"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1078\/1434\u20104610\u201000025"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.13933"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.protis.2010.09.003"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp348"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.13821"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2012.04.002"},{"key":"e_1_2_9_10_1","volume-title":"Illustrated Guide to Culture Collection of Free\u2010Living Amoebae","author":"Dykov\u00e1 I.","year":"2013"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.14411\/fp.2010.021"},{"key":"e_1_2_9_12_1","first-page":"81","article-title":"Amoebae Isolated From Organs of Farmed Tilapias, Oreochromis niloticus","volume":"44","author":"Dykov\u00e1 I.","year":"1997","journal-title":"Folia Parasitologica"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.14411\/fp.2005.040"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.12384"},{"key":"e_1_2_9_15_1","first-page":"415","article-title":"A Free\u2010Living Amoeba With Unusual Pattern of Mitochondrial Structure Isolated From Atlantic Salmon, Salmo salar L","volume":"41","author":"Dykov\u00e1 I.","year":"2002","journal-title":"Acta Protozoologica"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao03537"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.3354\/aei00325"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.13319"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejop.2018.10.003"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0014-3820.2002.tb00191.x"},{"key":"e_1_2_9_21_1","unstructured":"Hall T. 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