{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:26:49Z","timestamp":1791246409631,"version":"4.1.0"},"reference-count":51,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,5,7]],"date-time":"2025-05-07T00:00:00Z","timestamp":1746576000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001824","name":"Grantov\u00e1 Agentura \u010cesk\u00e9 Republiky","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2025,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Trematodes of the genus <jats:italic>Diplostomum<\/jats:italic> (Diplostomidae) are widely distributed and significant fish pathogens known for causing a range of negative effects. Any mechanism that protects the host from the parasite thus represents an evolutionary advantage. In this study, we observed an unusual localisation of <jats:italic>Diplostomum<\/jats:italic> metacercariae, which were displaced into the sac protruding from the lens, in North\u2010American bullhead catfish species of the genus <jats:italic>Ameiurus<\/jats:italic>. The occurrence of the sac was found in all sampling sites (Czech Republic) where <jats:styled-content style=\"fixed-case\"><jats:italic>A. nebulosus<\/jats:italic><\/jats:styled-content> and <jats:styled-content style=\"fixed-case\"><jats:italic>A. melas<\/jats:italic><\/jats:styled-content> were infected with <jats:italic>Diplostomum<\/jats:italic>, regardless of the parasite species, as both <jats:italic>D. pseudospathaceum<\/jats:italic> and <jats:styled-content style=\"fixed-case\"><jats:italic>D. spathaceum<\/jats:italic><\/jats:styled-content> induced sac formation. The probability of sac formation increased with the intensity of <jats:italic>Diplostomum<\/jats:italic> infection. Experimental infection of juvenile <jats:styled-content style=\"fixed-case\"><jats:italic>A. melas<\/jats:italic><\/jats:styled-content> showed that the first signs of sac formation appeared shortly after the infection. While visual observation under the light microscope suggested the first signs at 7\u2009days post\u2010infection, histological examination documented the start of epithelial proliferation by the third day post\u2010infection. Our study suggests that the process of sac formation, along with parasite displacement, might present a host reaction to mitigate the negative effects of infection and the impaired vision caused by the parasite.<\/jats:p>","DOI":"10.1111\/jfd.14137","type":"journal-article","created":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T09:41:45Z","timestamp":1748338905000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["<i>Diplostomum<\/i>\u2010Induced Sac Formation in Lenses of <i>Ameiurus<\/i> Bullheads: A Host Defence Response?"],"prefix":"10.1111","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0933-806X","authenticated-orcid":false,"given":"Mark\u00e9ta","family":"Ondra\u010dkov\u00e1","sequence":"first","affiliation":[{"name":"Institute of Vertebrate Biology of the Czech Academy of Sciences  Brno Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8313-1580","authenticated-orcid":false,"given":"Andrea","family":"Valigurov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Botany and Zoology Faculty of Science, Masaryk University  Brno Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3621-5460","authenticated-orcid":false,"given":"Iveta","family":"Hodov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Botany and Zoology Faculty of Science, Masaryk University  Brno Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1737-0668","authenticated-orcid":false,"given":"Veronika","family":"Bart\u00e1kov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Vertebrate Biology of the Czech Academy of Sciences  Brno Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2716-8375","authenticated-orcid":false,"given":"Maria Yu.","family":"Tkachenko","sequence":"additional","affiliation":[{"name":"Institute of Vertebrate Biology of the Czech Academy of Sciences  Brno Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6264-6091","authenticated-orcid":false,"given":"Michal","family":"Jan\u00e1\u010d","sequence":"additional","affiliation":[{"name":"Institute of Vertebrate Biology of the Czech Academy of Sciences  Brno Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0828-9684","authenticated-orcid":false,"given":"Luk\u00e1\u0161","family":"Vete\u0161n\u00edk","sequence":"additional","affiliation":[{"name":"Institute of Vertebrate Biology of the Czech Academy of Sciences  Brno Czech Republic"},{"name":"Department of Botany and Zoology Faculty of Science, Masaryk University  Brno Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,5,7]]},"reference":[{"key":"e_1_2_10_2_1","first-page":"37","article-title":"Parasites of Channel Catfish. Ictalurus punctatus Rafinesque, From the Island Region of Western Lake Erie","volume":"43","author":"Baker J. C.","year":"1976","journal-title":"Proceedings of the Helminthological Society of Washington"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0001\u2010706X(93)90079\u2010Q"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.2307\/3284227"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-540-29678-2_3178"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003\u20103472(80)80031\u20105"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2007.02.015"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11686\u2010024\u201000794\u20102"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000021284"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182021002092"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2022.109298"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2024.1392569"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2012.10.019"},{"issue":"1","key":"e_1_2_10_14_1","first-page":"29","article-title":"The Metacercaria of Diplostomum pseudospathaceum Niewiadomska, 1984 and \n                     Diplostomum spathaceum\n                   (Rudolphi, 1819) in the Ocular Lens of Fish and Reactions of the Lens to the Presence of the Parasites","volume":"34","author":"Graczyk T.","year":"1988","journal-title":"Wiadomo\u015bci Parazytologiczne"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2015.09.008"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/cne.900750206"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0021\u20105155(03)00075\u20103"},{"key":"e_1_2_10_18_1","volume-title":"Animal Tissue Techniques","author":"Humason G. L.","year":"1967"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/ece3.4151"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1079\/9781789243277.0434"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao01918"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1017\/s0031182004006006"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts199"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2016.1148"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/ece3.2645"},{"key":"e_1_2_10_26_1","doi-asserted-by":"publisher","DOI":"10.2307\/3221788"},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.2307\/3276087"},{"key":"e_1_2_10_28_1","first-page":"332","article-title":"Pathogenicity of Diplostomum Cercariae in Rainbow Trout, and Alternative Measures to Prevent Diplostomosis in Fish Farms","volume":"25","author":"Larsen A. H.","year":"2005","journal-title":"Bulletin of the European Association of Fish Pathologists"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.isci.2021.102533"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2015.07.001"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1093\/oso\/9780192895561.003.0014"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0166\u20106851(92)90181\u2010I"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1577\/1548\u20108667(1999)011<0275:DEFIYO>2.0.CO;2"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfb.12363"},{"key":"e_1_2_10_35_1","first-page":"1230","article-title":"Systematic Relationships Among Six Species of Diplostomum Nordmann, 1832 (Digenea) Based on Morphological and Molecular Data","volume":"47","author":"Niewiadomska K.","year":"2002","journal-title":"Acta Parasitologica"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2024.742100"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijppaw.2018.01.007"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1098\/rsbl.2006.0462"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00436\u2010016\u20105222\u2010z"},{"key":"e_1_2_10_40_1","first-page":"45","article-title":"Visual Ecology of Fish\u2014A Review With Special Reference to Percids","volume":"2","author":"Sandstr\u00f6m A.","year":"1999","journal-title":"Fiskeriverket Rapport"},{"key":"e_1_2_10_41_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756\u20133305\u20107\u2013109"},{"key":"e_1_2_10_42_1","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182021001372"},{"key":"e_1_2_10_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1095-8649.2008.02144.x"},{"key":"e_1_2_10_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anbehav.2005.01.021"},{"key":"e_1_2_10_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102761.1980.tb00432.x"},{"key":"e_1_2_10_46_1","unstructured":"Steenstrup J. J. S.1842.\u201c\u00dcber den Generationswechsel (Ubers. Lorenzen).\u201dXVII + 140pp. 3 pls. 80 Kopenhagen."},{"key":"e_1_2_10_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijppaw.2018.05.001"},{"key":"e_1_2_10_48_1","doi-asserted-by":"publisher","DOI":"10.3391\/bir.2022.11.1.27"},{"issue":"8","key":"e_1_2_10_49_1","first-page":"1819","article-title":"Thioacetamide\u2010Induced Cataract With Invasive Proliferation of the Lens Epithelium in Rainbow Trout","volume":"26","author":"Von Sallmann L.","year":"1966","journal-title":"Cancer Research"},{"key":"e_1_2_10_50_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1095\u20108649.2008.02050.x"},{"key":"e_1_2_10_51_1","doi-asserted-by":"publisher","DOI":"10.1645\/GE\u2010225R"},{"key":"e_1_2_10_52_1","doi-asserted-by":"publisher","DOI":"10.1177\/030098588702400613"}],"container-title":["Journal of Fish Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.14137","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,9,10]],"date-time":"2025-09-10T11:42:35Z","timestamp":1757504555000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jfd.14137"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,7]]},"references-count":51,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2025,10]]}},"alternative-id":["10.1111\/jfd.14137"],"URL":"https:\/\/doi.org\/10.1111\/jfd.14137","archive":["Portico"],"relation":{},"ISSN":["0140-7775","1365-2761"],"issn-type":[{"value":"0140-7775","type":"print"},{"value":"1365-2761","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5,7]]},"assertion":[{"value":"2024-12-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-05-07","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e14137"}}