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To date, no commercial fish vaccine is available in India to protect against streptococcosis due to a lack of proper adjuvants and vaccine targets. Multiple studies justify that immunomodulators like \u03b2\u2010glucan and Alkoxy glycerol (AKG) have improved immunity in fish. \u03b2\u2010glucan and AKG are natural homopolymers and lipids, respectively, which have proven immunostimulant capabilities in fish and can be effectively used as a prominent adjuvant to improve vaccine efficacy. The present study aims to demonstrate the improved adjuvant efficacy of the formalin\u2010inactivated\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>S. agalactiae<\/jats:italic>\n                    <\/jats:styled-content>\n                    vaccine. Nile tilapia fingerlings were immunised with formalin\u2010inactivated\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>S. agalactiae<\/jats:italic>\n                    <\/jats:styled-content>\n                    vaccines mixed with \u03b2\u2010glucan and AKG. Each week postvaccination, the serum nonspecific responses were measured by lysozyme, myeloperoxidase, SOD and bactericidal activity. Following the challenge after 28\u2009days postvaccination, the survival rate achieved was 32.14%, 57.14%, 53.57% and 71.43% in the respective experimental groups. The immune gene expressions of IgM, TCR\u03b2 and MHCII were evoked postvaccination. The specific serum IgM titre values were significantly upregulated in 14\u2010 and 28\u2010day postvaccinated experimental groups. The study highlights the enhanced activity of \u03b2\u2010glucan and AKG incorporated vaccine, which has the potency towards commercialisation for sustainable aquaculture.\n                  <\/jats:p>","DOI":"10.1111\/jfd.14141","type":"journal-article","created":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T03:30:07Z","timestamp":1747193407000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Immune Response and Protective Efficacy of \u03b2\u2010Glucan and Alkoxyglycerol as Adjuvant in\n                    <scp>\n                      <i>Streptococcus agalactiae<\/i>\n                    <\/scp>\n                    Formalin\u2010Inactivated Vaccine in Nile Tilapia (\n                    <scp>\n                      <i>Oreochromis niloticus<\/i>\n                    <\/scp>\n                    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