{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T04:52:04Z","timestamp":1780462324538,"version":"3.54.1"},"reference-count":100,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,5,29]],"date-time":"2025-05-29T00:00:00Z","timestamp":1748476800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100020469","name":"Universidad Cient\u00edfica del Sur","doi-asserted-by":"publisher","award":["student grant (beca cabieses)"],"award-info":[{"award-number":["student grant (beca cabieses)"]}],"id":[{"id":"10.13039\/100020469","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2025,11]]},"abstract":"ABSTRACT<\/jats:title>\n \n Aquaculture is one of the most important food sectors, providing nutrition and employment throughout the world. However, production is under threat due to the occurrence of pathogens.\n Aeromonas<\/jats:italic>\n spp. are bacterial agents that affect the aquaculture industry, including rainbow trout (\n \n Oncorhynchus mykiss<\/jats:italic>\n <\/jats:styled-content>\n ) farms, causing severe economic losses. The objective of this study was to characterise several isolates of\n Aeromonas<\/jats:italic>\n spp. obtained from rainbow trout in Jun\u00edn, Peru. Molecular analysis revealed the presence of four species:\n \n A. sobria<\/jats:italic>\n <\/jats:styled-content>\n (\n n<\/jats:italic>\n \u2009=\u200912),\n \n A. salmonicida<\/jats:italic>\n <\/jats:styled-content>\n (\n n<\/jats:italic>\n \u2009=\u20093),\n \n A. popoffii<\/jats:italic>\n <\/jats:styled-content>\n (\n n<\/jats:italic>\n \u2009=\u20092), and\n \n A. media<\/jats:italic>\n <\/jats:styled-content>\n (\n n<\/jats:italic>\n \u2009=\u20091). Biochemical characterisation revealed that several isolates tolerated 3% NaCl, with only\n \n A. salmonicida<\/jats:italic>\n <\/jats:styled-content>\n surviving in 4% NaCl. Virulence factor analysis demonstrated increased virulence at higher temperatures for\n \n A. sobria<\/jats:italic>\n <\/jats:styled-content>\n , while\n \n A. popoffii<\/jats:italic>\n <\/jats:styled-content>\n exhibited virulence in colder conditions.\n \n A. media<\/jats:italic>\n <\/jats:styled-content>\n displayed limited virulence properties, while\n \n A. salmonicida<\/jats:italic>\n <\/jats:styled-content>\n was consistently virulent across all temperatures.\n \n Aeromonas salmonicida<\/jats:italic>\n <\/jats:styled-content>\n isolates exhibited intense and prolonged motility\u2014a behaviour rarely associated with this species\u2014that persisted across a broad temperature range from 14\u00b0C to 37\u00b0C. The virulence genes\n aer<\/jats:italic>\n ,\n fla<\/jats:italic>\n ,\n ahy<\/jats:italic>\n B,\n lip<\/jats:italic>\n ,\n act<\/jats:italic>\n ,\n ser<\/jats:italic>\n ,\n hly<\/jats:italic>\n and\n altA<\/jats:italic>\n were present in all\n \n A. salmonicida<\/jats:italic>\n <\/jats:styled-content>\n isolates, with both\n \n A. popoffii<\/jats:italic>\n <\/jats:styled-content>\n isolates lacking only the\n fla<\/jats:italic>\n gene. Experimental infection resulted in mortality of up to 100% for\n \n A. salmonicida<\/jats:italic>\n <\/jats:styled-content>\n (7.2\u2009\u00d7\u200910\n 6<\/jats:sup>\n and 7.2\u2009\u00d7\u200910\n 7<\/jats:sup>\n \u2009CFU\/fish), 40% for\n \n A. sobria<\/jats:italic>\n <\/jats:styled-content>\n (4\u2009\u00d7\u200910\n 7<\/jats:sup>\n \u2009CFU\/fish), and 20% for\n \n A. popoffii<\/jats:italic>\n <\/jats:styled-content>\n (4\u2009\u00d7\u200910\n 7<\/jats:sup>\n \u2009CFU\/fish). No direct relation was found between specific gene presence or combinations thereof and mortality. Our findings highlight the need to develop effective management strategies, including the development of a multivalent vaccine to enhance Peruvian trout aquaculture sustainability.\n <\/jats:p>","DOI":"10.1111\/jfd.14158","type":"journal-article","created":{"date-parts":[[2025,5,29]],"date-time":"2025-05-29T08:26:05Z","timestamp":1748507165000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Isolation, Characterisation and Virulence of\n Aeromonas<\/i>\n spp. From Peruvian Rainbow Trout: First Description of\n A. popoffii<\/i>\n \u2010Associated Mortality"],"prefix":"10.1111","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-2662-4977","authenticated-orcid":false,"given":"Christopher James","family":"Engert","sequence":"first","affiliation":[{"name":"Universidad Cient\u00edfica del Sur Lima 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