{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T19:30:13Z","timestamp":1789587013030,"version":"build-2803163510"},"reference-count":37,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T00:00:00Z","timestamp":1751328000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100008725","name":"Agencia Nacional de Investigaci\u00f3n e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["FONDECYT1190283"],"award-info":[{"award-number":["FONDECYT1190283"]}],"id":[{"id":"10.13039\/100008725","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008725","name":"Agencia Nacional de Investigaci\u00f3n e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["FONDAPINCAR1523A0007"],"award-info":[{"award-number":["FONDAPINCAR1523A0007"]}],"id":[{"id":"10.13039\/100008725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2026,1]]},"abstract":"ABSTRACT<\/jats:title>\n \n Tenacibaculosis, caused by\n \n T. dicentrarchi<\/jats:italic>\n <\/jats:styled-content>\n , results in skin lesions, ulceration, yellow plaques on teeth, and haemorrhaging in the operculum, peduncle and pectoral fins. It is the second leading cause of mortality in farmed Atlantic salmon (\n \n Salmo salar<\/jats:italic>\n <\/jats:styled-content>\n ) in Chile. Currently, no vaccine is available to prevent the disease.\n In silico<\/jats:italic>\n and in\u00a0vivo studies have shown that iron plays a key role in\n \n T. dicentrarchi<\/jats:italic>\n <\/jats:styled-content>\n infection. We hypothesised that culturing the TdCh05 vaccine strain under iron\u2010limiting conditions (i.e., with non\u2010assimilable iron chelator 2.2\u2032\u2010dipyridyl [DIP]) would enhance Atlantic salmon protection against tenacibaculosis. Fish were vaccinated intraperitoneally with prototypes (A) TdCh05, (B) TdCh05\u2009+\u2009DIP or (C) A\u2009+\u2009B (a 1:1 mixture of inactivated cultures), and a control group was challenged by bath immersion with a heterologous\n \n T. dicentrarchi<\/jats:italic>\n <\/jats:styled-content>\n strain. Each prototype was emulsified with a commercial adjuvant. At 14\u2009days post\u2010challenge, Atlantic salmon injected with FMM broth had a cumulative mortality of 73.3%, followed by the TdCh05\u2009+\u2009DIP (66.7%) and A\u2009+\u2009B (54.8%) prototypes. The lowest cumulative mortality value (50%) was observed for the prototype containing\n \n T. dicentrarchi<\/jats:italic>\n <\/jats:styled-content>\n TdCh05 grown only in FMM. Moreover, a high proportion of the vaccinated fish that survived the challenge, regardless of the vaccine prototype, carried\n \n T. dicentrarchi<\/jats:italic>\n <\/jats:styled-content>\n in various internal organs, particularly in the spleen. Future studies should focus on identifying the most suitable antigens to develop an effective vaccine for the prevention of\n \n T. dicentrarchi<\/jats:italic>\n <\/jats:styled-content>\n \u2010induced tenacibaculosis.\n <\/jats:p>","DOI":"10.1111\/jfd.70013","type":"journal-article","created":{"date-parts":[[2025,7,1]],"date-time":"2025-07-01T06:09:44Z","timestamp":1751350184000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Efficacy of a Vaccine for Atlantic Salmon (\n Salmo salar<\/i>\n ) Using a\n Tenacibaculum dicentrarchi<\/i>\n Strain Cultured Under Iron\u2010Limited Conditions"],"prefix":"10.1111","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5368-4475","authenticated-orcid":false,"given":"Ruben","family":"Avenda\u00f1o\u2010Herrera","sequence":"first","affiliation":[{"name":"Laboratorio de Patolog\u00eda de Organismos Acu\u00e1ticos y Biotecnolog\u00eda Acu\u00edcola Facultad de Ciencias de la Vida, Universidad Andr\u00e9s Bello Vi\u00f1a del Mar Chile"},{"name":"Centro Interdisciplinario Para la Investigaci\u00f3n en Acuicultura (INCAR), Universidad Andr\u00e9s Bello Santiago Chile"},{"name":"Centro de Investigaci\u00f3n Marina Quintay (CIMARQ), Universidad Andr\u00e9s Bello Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rute","family":"Irgang","sequence":"additional","affiliation":[{"name":"Laboratorio de Patolog\u00eda de Organismos Acu\u00e1ticos y Biotecnolog\u00eda Acu\u00edcola Facultad de Ciencias de la Vida, Universidad Andr\u00e9s Bello Vi\u00f1a del Mar Chile"},{"name":"Centro Interdisciplinario Para la Investigaci\u00f3n en Acuicultura (INCAR), Universidad Andr\u00e9s Bello Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henry","family":"Araya\u2010Le\u00f3n","sequence":"additional","affiliation":[{"name":"Laboratorio de Patolog\u00eda de Organismos Acu\u00e1ticos y Biotecnolog\u00eda Acu\u00edcola Facultad de Ciencias de la Vida, Universidad Andr\u00e9s Bello Vi\u00f1a del Mar Chile"},{"name":"Centro Interdisciplinario Para la Investigaci\u00f3n en Acuicultura (INCAR), Universidad Andr\u00e9s Bello Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pedro","family":"Ilardi","sequence":"additional","affiliation":[{"name":"Cooperative Program for Aquaculture, Universidad de Chile, Universidad Cat\u00f3lica del Norte, Pontificia Universidad Cat\u00f3lica de Valpara\u00edso Valpara\u00edso Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ra\u00fal","family":"Cort\u00e9s","sequence":"additional","affiliation":[{"name":"Centro Interdisciplinario Para la Investigaci\u00f3n en Acuicultura (INCAR), Universidad Andr\u00e9s Bello Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7]]},"reference":[{"key":"e_1_2_9_2_1","first-page":"447","article-title":"Potency Testing of Fish Vaccines","volume":"49","author":"Amend D. 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