{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T06:31:54Z","timestamp":1787725914406,"version":"build-2784847793"},"reference-count":32,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,6,30]],"date-time":"2025-06-30T00:00:00Z","timestamp":1751241600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    A lateral flow immunochromatographic assay (LFIA) was developed for the rapid detection of infectious haematopoietic necrosis virus (IHNV, genogroups JRt\u2010N and JRt\u2010S) in rainbow trout (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Oncorhynchus mykiss<\/jats:italic>\n                    <\/jats:styled-content>\n                    ). The assay utilises the monoclonal antibody (mAb) IHNV\u20103, which serves as both the detector antibody in a colloidal gold\u2010mAb conjugate and the capture antibody on the test line. The LFIA strip demonstrated a detection limit ranging from 10\n                    <jats:sup>5.05<\/jats:sup>\n                    to 10\n                    <jats:sup>5.8<\/jats:sup>\n                    TCID\n                    <jats:sub>50<\/jats:sub>\n                    \/100\u2009\u03bcL for six IHNV isolates (genogroups JRt\u2010N and JRt\u2010S) and showed no cross\u2010reactivity with other fish viruses. Field validation using spleen samples from 81 IHNV\u2010infected and 102 non\u2010infected rainbow trout revealed a specificity of 100%, a sensitivity of 97.5%, and an overall diagnostic effectiveness of 98.9%. These results highlight the LFIA strip as a reliable tool for the rapid (within 10\u2009min) on\u2010site detection of IHNV in rainbow trout at aquaculture facilities.\n                  <\/jats:p>","DOI":"10.1111\/jfd.70014","type":"journal-article","created":{"date-parts":[[2025,6,30]],"date-time":"2025-06-30T05:49:18Z","timestamp":1751262558000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Field\u2010Usable Lateral Flow Immunochromatographic Assay for Rapid Detection of Infectious Haematopoietic Necrosis Virus (\n                    <scp>IHNV<\/scp>\n                    )"],"prefix":"10.1111","volume":"49","author":[{"given":"Choon\u2010Sup","family":"Kim","sequence":"first","affiliation":[{"name":"EnBioGene Inc.  Yeosu Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-3707-2900","authenticated-orcid":false,"given":"Kyoung\u2010Hui","family":"Kong","sequence":"additional","affiliation":[{"name":"Pathology Division National Institute of Fisheries Science (NIFS)  Busan Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-3391-2462","authenticated-orcid":false,"given":"Sung\u2010Hun","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Fishery, Marine, Industry, Tourism, and Leisure Chonnam National University  Yeosu Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wi\u2010Sik","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Aqualife Medicine Chonnam National University  Yeosu Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,6,30]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2024.115008"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13567\u2010016\u20100341\u20101"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao040163"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2015.08.032"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2023.199133"},{"key":"e_1_2_10_7_1","first-page":"81","article-title":"Isolation and Characterization of Infectious Hematopoietic Necrosis Virus Causing High Mortality in Rainbow Trout Oncorhynchus mykiss","volume":"16","author":"Kim K. 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