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Conventional reliance on antibiotics to combat these infections has raised serious concerns regarding antimicrobial resistance, environmental contamination and food safety. Additionally, environmental stressors such as salinity shifts and poor water quality exacerbate disease outbreaks, leading to severe production losses across Asia and Latin America. To explore eco\u2010friendly therapeutic alternatives, this study assessed the antiviral potential of cannabidiol (CBD), a bioactive compound extracted from\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Cannabis sativa<\/jats:italic>\n                    <\/jats:styled-content>\n                    seed oil, identified through GC\u2013MS analysis. Using molecular docking techniques, we evaluated CBD's interactions with key viral proteins: VP28 of WSSV, the tubulin \u03b2\u2010chain of EHP and the capsid protein of IHHNV. The docking results revealed strong binding affinities of \u22126.61\u2009kcal\/mol (EHP), \u22126.72\u2009kcal\/mol (IHHNV) and \u22125.38\u2009kcal\/mol (WSSV), indicating stable and potentially inhibitory interactions. Structural models were retrieved from RCSB PDB and SwissModel, while ligand preparation and docking were performed using AutoDock 4.2. CBD also demonstrated favourable pharmacokinetic and safety profiles, with predictions indicating no mutagenicity, hepatotoxicity or cardiotoxicity, and acceptable drug\u2010likeness characteristics. Compared to other plant\u2010derived compounds previously tested in shrimp disease models, CBD exhibited superior binding stability, more interaction residues and better bioavailability scores. These findings highlight CBD as a promising dual\u2010function agent, capable of both modulating shrimp immunity and directly inhibiting key viral pathogens. These findings highlight cannabidiol (CBD) as a promising dual\u2010action compound, with the potential to both enhance shrimp immune responses and exert direct antiviral effects against key pathogens. This study lays a robust groundwork for future in\u00a0vivo validations, formulation strategies and regulatory frameworks, ultimately supporting the development of sustainable, precision\u2010based aquaculture health management.\n                  <\/jats:p>","DOI":"10.1111\/jfd.70015","type":"journal-article","created":{"date-parts":[[2025,7,14]],"date-time":"2025-07-14T11:23:29Z","timestamp":1752492209000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["In Silico Assessment of Cannabidiol From\n                    <scp>\n                      <i>Cannabis sativa<\/i>\n                    <\/scp>\n                    as an Antiviral Agent Against Key Shrimp Pathogens in Aquaculture"],"prefix":"10.1111","volume":"49","author":[{"given":"Shoba","family":"Gunasekaran","sequence":"first","affiliation":[{"name":"Department of Biotechnology Dwaraka Doss Goverdhan Doss Vaishnav College  Chennai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-8719-2632","authenticated-orcid":false,"given":"Atchuthan","family":"Purushothaman","sequence":"additional","affiliation":[{"name":"Sathyabama Institute of Science and Technology  Chennai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Anju","sequence":"additional","affiliation":[{"name":"Sathyabama Institute of Science and Technology  Chennai India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,7,14]]},"reference":[{"issue":"2","key":"e_1_2_13_2_1","first-page":"1024","article-title":"An Overview of Molecular Docking","volume":"4","author":"Agarwal S.","year":"2016","journal-title":"JSM Chemistry"},{"key":"e_1_2_13_3_1","unstructured":"Akpolat N. andY. 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