{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T03:24:54Z","timestamp":1788578694460,"version":"build-2803163510"},"reference-count":36,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,7,17]],"date-time":"2025-07-17T00:00:00Z","timestamp":1752710400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,7,17]],"date-time":"2025-07-17T00:00:00Z","timestamp":1752710400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2026,2]]},"abstract":"ABSTRACT<\/jats:title>\n \n The in\u00a0vitro efficacy of various anthelminthic agents was investigated against adults and eggs of the\n \n Microcotyle sebastis<\/jats:italic>\n <\/jats:styled-content>\n and\n \n M. caudata<\/jats:italic>\n <\/jats:styled-content>\n . Praziquantel (PZQ), ivermectin (IVM), trichlorfon (TCF), fenbendazole (FNBZ), febantel (FBT), flubendazole (FLBZ), thiabendazole (TBZ), formalin, hydrogen peroxide and sodium hypochlorite (NaOCl) were selected as test agents. The hatching rate of\n \n M. sebastis<\/jats:italic>\n <\/jats:styled-content>\n and\n \n M. caudata<\/jats:italic>\n <\/jats:styled-content>\n eggs was significantly reduced by formalin (200, 300\u2009mg\/L), hydrogen peroxide (50\u2013200\u2009mg\/L) and NaOCl (75\u2013200\u2009mg\/L) treatment. However, seven anthelminthic agents, including PZQ, did not effectively inhibit the hatching of eggs. 50\u2013200\u2009mg\/L NaOCl treatment significantly killed\n \n M. sebastis<\/jats:italic>\n <\/jats:styled-content>\n and\n \n M. caudata<\/jats:italic>\n <\/jats:styled-content>\n adults. In particular, IVM 200\u2009mg\/L for 30\u2009min treatment showed 100% killing activity against\n \n M. sebastis<\/jats:italic>\n <\/jats:styled-content>\n adults and 80% killing activity against\n \n M. caudata<\/jats:italic>\n <\/jats:styled-content>\n adults. However, PZQ, TCF, FNBZ, FBT, FLBZ and TBZ treatments showed mortalities of less than 33%. When anthelmintics were directly applied to adult worms attached to the gills in\u00a0vitro, PZQ 200\u2009mg\/L for 30\u2009min treatment induced detachment of\n \n M. sebastis<\/jats:italic>\n <\/jats:styled-content>\n (99.2%) and\n \n M. caudata<\/jats:italic>\n <\/jats:styled-content>\n (100%) from the gills. However, most of the worms recovered after the treatment. IVM 200\u2009mg\/L induced detachment of more than 83% of\n \n M. sebastis<\/jats:italic>\n <\/jats:styled-content>\n and\n \n M. caudata<\/jats:italic>\n <\/jats:styled-content>\n on the gills and, consequently, death of the detached worms. All the other agents induced negligible effects on the adult worms attached to the gills. This result suggests that NaOCl and IVM bath treatments effectively control\n \n M. sebastis<\/jats:italic>\n <\/jats:styled-content>\n and\n \n M. caudata<\/jats:italic>\n <\/jats:styled-content>\n in sebastid species aquaculture, and formalin, hydrogen peroxide and NaOCl are effective against eggs. An in\u00a0vivo study will be necessary to evaluate their anthelmintic effects on aquaculture farms.\n <\/jats:p>","DOI":"10.1111\/jfd.70024","type":"journal-article","created":{"date-parts":[[2025,7,17]],"date-time":"2025-07-17T08:33:30Z","timestamp":1752741210000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["In Vitro Anthelmintic Effect of Selected Agents Against\n Microcotyle sebastis<\/i>\n and\n Microcotyle caudata<\/i>\n , the Gill Monogenean Parasites of Rockfish,\n Sebastes<\/i>\n spp."],"prefix":"10.1111","volume":"49","author":[{"given":"U\u2010Hwa","family":"Nam","sequence":"first","affiliation":[{"name":"Department of Aquatic Life Medicine Gangneung\u2010Wonju National University Gangneung Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han\u2010Seul","family":"Cho","sequence":"additional","affiliation":[{"name":"Department of Aquatic Life Medicine Gangneung\u2010Wonju National University Gangneung Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7920-8258","authenticated-orcid":false,"given":"Jeong\u2010Ho","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Aquatic Life Medicine Gangneung\u2010Wonju National University Gangneung Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,17]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.12952"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.13725"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/jvp.12735"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaeng.2020.102140"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1017\/s0031182022001093"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365\u20102109.2000.00510.x"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0020\u20107519(93)90053\u20102"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.12089"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2018.08.070"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2013.04.021"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.3147\/jsfp.55.80"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10499\u2010019\u201000471\u20106"},{"key":"e_1_2_9_14_1","first-page":"272","volume-title":"Fish Parasites: A Handbook of Protocols for Their Isolation, Culture and Transmission","author":"Hutson K. 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