{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T15:30:40Z","timestamp":1787671840123,"version":"build-2736575974"},"reference-count":55,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,8,2]],"date-time":"2025-08-02T00:00:00Z","timestamp":1754092800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,8,2]],"date-time":"2025-08-02T00:00:00Z","timestamp":1754092800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF\u20102022R1I1A2068984"],"award-info":[{"award-number":["NRF\u20102022R1I1A2068984"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Many studies have demonstrated that miRNAs play a crucial role in virus\u2010host interactions. This study aimed to investigate the antiviral effects of miR\u2010146a in Epithelioma papulosum cyprini (EPC) cells infected with viral hemorrhagic septicemia virus (VHSV), infectious pancreatic necrosis virus (IPNV), and infectious haematopoietic necrosis virus (IHNV), which are major causes of mortality in various freshwater and marine fish worldwide. Cytopathic effects (CPE) were observed as early as 72\u2009h post\u2010infection in both the control group and the miR\u2010146a inhibitor group. In contrast, CPE formation was not observed in virus\u2010infected groups treated with the miR\u2010146a mimic. The control and miR\u2010146a inhibitor groups exhibited a rapid increase in viral titres after 48\u2009h, whereas the miR\u2010146a mimic\u2010treated groups showed minimal viral replication at all assay time points. To further investigate the antiviral mechanism of miR\u2010146a, we analysed the genes upregulated by miR\u2010146a following viral stimulation. The results revealed a significant increase in the expression of genes involved in the type I interferon (IFN) response in the miR\u2010146a mimic group. Taken together, these findings suggest that miR\u2010146a exerts antiviral effects against VHSV, IPNV and IHNV.<\/jats:p>","DOI":"10.1111\/jfd.70025","type":"journal-article","created":{"date-parts":[[2025,8,2]],"date-time":"2025-08-02T07:29:17Z","timestamp":1754119757000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Antiviral Effect of\n                    <scp>miR<\/scp>\n                    \u2010146a in Epithelioma Papulosum Cyprini (\n                    <scp>EPC<\/scp>\n                    ) Cells Against Three Fish Viruses:\n                    <scp>VHSV<\/scp>\n                    ,\n                    <scp>IHNV<\/scp>\n                    and\n                    <scp>IPNV<\/scp>"],"prefix":"10.1111","volume":"49","author":[{"given":"Yoon\u2010Hwan","family":"Jeon","sequence":"first","affiliation":[{"name":"Department of Biological Sciences Kongju National University  Gongju South Korea"},{"name":"Institute of Vision Research Department of Ophthalmology, Severance Eye Hospital Severance Eye Hospital, Yonsei University College of Medicine  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ha Eun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Kongju National University  Gongju South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Najib","family":"Abdellaoui","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Kongju National University  Gongju South Korea"},{"name":"Biotechnology Research Institute Kongju National University  Gongju South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5935-0249","authenticated-orcid":false,"given":"Min Sun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Kongju National University  Gongju South Korea"},{"name":"Biotechnology Research Institute Kongju National University  Gongju South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,8,2]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2023.108937"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10126\u2010024\u201010354\u20104"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2022.738000"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2016.12.013"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1261\/rna.2183803"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1577\/1548\u20108667(200006)012<0085:MEREOA>2.0.CO;2"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2014.07.003"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2005.08.087"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2016.02.012"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2017.08.073"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2005.06.036"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2017.01.011"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13567\u2010016\u20100341\u20101"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0959\u20108030(95)00003\u20108"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.3390\/v14102184"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagrm.2008.05.005"},{"key":"e_1_2_9_18_1","unstructured":"Hambrey J.2017.\u201cThe 2030 Agenda and the Sustainable Development Goals: The Challenge for Aquaculture Development and Management. FAO Fisheries and Aquaculture Circular (C1141).\u201d"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1006\/viro.2001.1336"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagrm.2011.05.009"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1639"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010022\u201007346\u20106"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao047087"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1113329"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.12317"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.2962"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2018.12.008"},{"key":"e_1_2_9_28_1","volume-title":"Aquaculture Virology","author":"Kibenge F. S.","year":"2024"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2023.199133"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.3390\/v6052204"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2016.09.035"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2009.07.019"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010marine\u2010010814\u2010015646"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1108784"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.02203\u201009"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2012.11.002"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1002\/iub.2222"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2016.03.014"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1099\/jgv.0.000811"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.prevetmed.2009.11.020"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1080\/13657305.2012.678558"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virol.2011.06.014"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.180.8.5689"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2012.63"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0912986107"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2009.03.004"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.00456\u201010"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.01342\u201012"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102761.2008.00951.x"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.049528\u20100"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.22158"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2013.05.003"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1800377"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.01594\u201017"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep28354"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1002\/vms3.931"}],"container-title":["Journal of Fish Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.70025","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jfd.70025","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.70025","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T14:59:41Z","timestamp":1787669981000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jfd.70025"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,2]]},"references-count":55,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["10.1111\/jfd.70025"],"URL":"https:\/\/doi.org\/10.1111\/jfd.70025","archive":["Portico"],"relation":{},"ISSN":["0140-7775","1365-2761"],"issn-type":[{"value":"0140-7775","type":"print"},{"value":"1365-2761","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,2]]},"assertion":[{"value":"2025-04-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-08","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-02","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70025"}}