{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T15:54:09Z","timestamp":1776095649547,"version":"3.50.1"},"reference-count":49,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,7,14]],"date-time":"2025-07-14T00:00:00Z","timestamp":1752451200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32202993"],"award-info":[{"award-number":["32202993"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Gyrodactylus<\/jats:italic>\n                    spp. pose significant threats to farmed fish due to their high pathogenicity, resulting in substantial economic losses in aquaculture. Currently, treatments for gyrodactylosis primarily rely on chemical agents, the efficacy and safety of which are not always satisfactory, underscoring the urgent need for novel anthelmintic agents. In the pursuit of novel therapeutic agents, we assessed the in\u00a0vivo anthelmintic efficacy of 20 natural compounds using a goldfish (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Carassius auratus<\/jats:italic>\n                    <\/jats:styled-content>\n                    )\u2013\n                    <jats:italic>Gyrodactylus kobayashii<\/jats:italic>\n                    infection model. Among these compounds, naringenin (NAR) exhibited the highest efficacy and was selected for further investigation. In\u00a0vitro results demonstrated NAR's significant anti\u2010parasitic activity, achieving 100% mortality at 4.0\u2009mg\/L after 60\u2009min of exposure. Subsequent in\u00a0vivo investigations revealed that the median effective concentration (EC\n                    <jats:sub>50<\/jats:sub>\n                    ) of NAR was 0.859\u2009mg\/L at 24\u2009h and 0.704\u2009mg\/L at 48\u2009h post\u2010treatment, with EC\n                    <jats:sub>100<\/jats:sub>\n                    at 2\u2009mg\/L. Additionally, NAR significantly impaired the mobility of\n                    <jats:italic>G.\u2009kobayashii<\/jats:italic>\n                    . Confocal microscopy revealed that NAR\u2010induced structural damage in worm tissues, including epidermal disruption and muscle dissolution. Moreover, both in\u00a0vitro and in\u00a0vivo exposure to NAR caused a decline in ATP levels, indicating potential impairment of energy production in\n                    <jats:italic>G.\u2009kobayashii<\/jats:italic>\n                    . Collectively, these findings establish NAR as a promising therapeutic agent for protecting farmed fish against\n                    <jats:italic>Gyrodactylus<\/jats:italic>\n                    infections.\n                  <\/jats:p>","DOI":"10.1111\/jfd.70026","type":"journal-article","created":{"date-parts":[[2025,7,14]],"date-time":"2025-07-14T10:49:13Z","timestamp":1752490153000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Naringenin as a Promising Therapeutic Agent for\n                    <i>Gyrodactylus kobayashii<\/i>\n                    in Goldfish"],"prefix":"10.1111","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-1951-682X","authenticated-orcid":false,"given":"Guangshuo","family":"Wang","sequence":"first","affiliation":[{"name":"Hainan Institute of Northwest A&amp;F University  Sanya Hainan China"},{"name":"College of Animal Science and Technology Northwest A&amp;F University  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Yangling Shaanxi China"},{"name":"Engineering Research Center of the Innovation and Development of Green Fishery Drugs Universities of Shaanxi Province, Northwest A&amp;F University  Yangling Shaanxi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9465-108X","authenticated-orcid":false,"given":"Gaoxue","family":"Wang","sequence":"additional","affiliation":[{"name":"Hainan Institute of Northwest A&amp;F University  Sanya Hainan China"},{"name":"College of Animal Science and Technology Northwest A&amp;F University  Yangling Shaanxi China"},{"name":"Key Laboratory of Livestock Biology Northwest A&amp;F University  Yangling Shaanxi China"},{"name":"Engineering Research Center of the Innovation and Development of Green Fishery Drugs Universities of Shaanxi Province, Northwest A&amp;F University  Yangling Shaanxi 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