{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T17:52:37Z","timestamp":1790790757821,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,7,29]],"date-time":"2025-07-29T00:00:00Z","timestamp":1753747200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,7,29]],"date-time":"2025-07-29T00:00:00Z","timestamp":1753747200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004410","name":"T\u00fcrkiye Bilimsel ve Teknolojik Ara\u015ft\u0131rma Kurumu","doi-asserted-by":"publisher","award":["222O736"],"award-info":[{"award-number":["222O736"]}],"id":[{"id":"10.13039\/501100004410","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Diagnosis time of infectious diseases in aquaculture is an important issue, and local and commercial enterprises generally do not prefer laboratory diagnosis of infectious diseases due to the long lead time and cost. This leads to the under\u2010reporting of outbreaks in aquaculture farms and the recognition of infectious agents when they spread to many farm systems and cause significant economic loss. In this study, we aim to develop a fast and simple diagnostic test that can easily be applied under field conditions against Infectious pancreatic necrosis virus (IPNV) and Viral hemorrhagic septicemia virus (VHSV Ie), which are two crucial viral pathogens that cause significant problems in trout farming in T\u00fcrkiye, both in hatcheries and farms. For this reason, we developed a lateral flow test strip for the duplex diagnosis of VHSV Ie and IPNV sp. based on Recombinase polymerase amplification (RPA), one of the molecular tests developed in recent years. We also compared Quantitative RT\u2010PCR (q RT\u2010PCR), Quantitative RT\u2010RPA (rtRT\u2010RPA) and lateral flow RT\u2010RPA (lfRT\u2010RPA) methods, which optimise both singleplex and duplex. As a result of this stage, the minimum detection limits of both rtRT\u2010PCR, rtRT\u2010RPA, and lfRT\u2010RPA methods were determined. The lfRT\u2010RPA test is almost as sensitive as rtRT\u2010PCR and rtRT\u2010RPA for the detection of duplex VHSV Ie, but for the detection of the IPNV sp., the lfRT\u2010RPA test was found to be approximately 10\u2010fold less sensitive than rtRT\u2010PCR and rtRT\u2010RPA. It is thought that the duplex lateral flow detection kit will allow timely diagnosis in the field for detecting infectious diseases of other aquaculture farms.<\/jats:p>","DOI":"10.1111\/jfd.70029","type":"journal-article","created":{"date-parts":[[2025,7,29]],"date-time":"2025-07-29T09:05:00Z","timestamp":1753779900000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Implementation of a Recombinase Polymerase Amplification\u2010Driven Duplex Lateral Flow Diagnostic Kit for Concurrent Detecting Infectious Pancreatic Necrosis Virus and Viral Hemorrhagic Septicemia Virus: A Comparative Analysis of\n                    <scp>RPA<\/scp>\n                    and\n                    <scp>PCR<\/scp>\n                    Methods"],"prefix":"10.1111","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3240-8425","authenticated-orcid":false,"given":"Cuneyt","family":"Tamer","sequence":"first","affiliation":[{"name":"Faculty of Veterinary Medicine, Department of Virology Ondokuz May\u0131s University  Samsun Turkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanne Nur","family":"Kuru\u00e7ay","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Department of Virology Ondokuz May\u0131s University  Samsun Turkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seda","family":"G\u00f6zel","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Department of Virology Ondokuz May\u0131s University  Samsun Turkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bahad\u0131r","family":"M\u00fcftoglu","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Department of Experimental Animals Ondokuz May\u0131s University  Samsun Turkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-4075-7427","authenticated-orcid":false,"given":"Elif Nur","family":"K\u00f6kluo\u011flu","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Department of Virology Ondokuz May\u0131s University  Samsun Turkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamza","family":"Kadi","sequence":"additional","affiliation":[{"name":"Republic of T\u00fcrkiye, Ministry of Agriculture and Forestry Samsun Veterinary Control Institute  Samsun T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatih","family":"Dogan","sequence":"additional","affiliation":[{"name":"Republic of T\u00fcrkiye, Ministry of Agriculture and Forestry Samsun Veterinary Control Institute  Samsun T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Serdar","family":"Yildirim","sequence":"additional","affiliation":[{"name":"Republic of T\u00fcrkiye, Ministry of Agriculture and Forestry Samsun Veterinary Control Institute  Samsun T\u00fcrkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Semra","family":"Gumusova","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Department of Virology Ondokuz May\u0131s University  Samsun Turkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2806-7878","authenticated-orcid":false,"given":"Zafer","family":"Yazici","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Department of Virology Ondokuz May\u0131s University  Samsun Turkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7063-7491","authenticated-orcid":false,"given":"Manfred","family":"Weidmann","sequence":"additional","affiliation":[{"name":"Medizinische Hochschule BrandenburgTheodor Fontane  Senftenberg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4468-2790","authenticated-orcid":false,"given":"Harun","family":"Albayrak","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine, Department of Virology Ondokuz May\u0131s University  Samsun Turkiye"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,7,29]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0071642"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2016.01.005"},{"issue":"1","key":"e_1_2_9_4_1","first-page":"67","article-title":"Genetic Analysis of the Complete G Gene of Viral Hemorrhagic Septicemia Virus (VHSV) Genotype Ie Isolates From Turkey","volume":"17","author":"Albayrak H.","year":"2018","journal-title":"Iranian Journal of Fisheries Sciences"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2013.06.027"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.574231"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2015.245829"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2019.03.015"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/978\u20101\u20100716\u20100581\u20103_14"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.22392\/actaquatr.1594067"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fchem.2021.815189"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.22092\/ijfs.2019.119829"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.2807\/1560\u20107917.ES.2015.20.44.30053"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102761.2011.01264.x"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.12910"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2021.635185"},{"key":"e_1_2_9_17_1","unstructured":"ICTV.2025.\u201cInternational Committee on Taxonomy of Viruses Current Current ICTV Taxonomy Release.\u201dhttps:\/\/ictv.global\/taxonomy."},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705\u2010020\u201004798\u2010x"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102761.2012.01416.x"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006862304381"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2024.1379939"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2017.10.015"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12560\u2010011\u20109062\u20109"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.12237"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.72.4.2373\u20102378.2006"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.01984"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0040204"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10499\u2010021\u201000806\u20102"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2021.113771"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.13629"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2021.06.002"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/are.14994"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.13558"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/aje\/kwk052"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ab.2022.114627"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.13492"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.7b00964"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.01064\u201024"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2020.736338"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2021.114343"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2021.114094"}],"container-title":["Journal of Fish Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.70029","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jfd.70029","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.70029","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T14:59:32Z","timestamp":1787669972000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jfd.70029"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,29]]},"references-count":40,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["10.1111\/jfd.70029"],"URL":"https:\/\/doi.org\/10.1111\/jfd.70029","archive":["Portico"],"relation":{},"ISSN":["0140-7775","1365-2761"],"issn-type":[{"value":"0140-7775","type":"print"},{"value":"1365-2761","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,29]]},"assertion":[{"value":"2025-05-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-18","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-29","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70029"}}