{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T00:13:04Z","timestamp":1791591184276,"version":"4.3.4"},"reference-count":102,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,8,5]],"date-time":"2025-08-05T00:00:00Z","timestamp":1754352000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,8,5]],"date-time":"2025-08-05T00:00:00Z","timestamp":1754352000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100009037","name":"Science and Technology Research Partnership for Sustainable Development","doi-asserted-by":"publisher","award":["JPMJSA1806"],"award-info":[{"award-number":["JPMJSA1806"]}],"id":[{"id":"10.13039\/501100009037","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Edwardsiella piscicida<\/jats:italic>\n                    is a significant pathogen that poses a particular threat to Japanese flounder (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Paralichthys olivaceus<\/jats:italic>\n                    <\/jats:styled-content>\n                    ) aquaculture in Japan and other countries. The damage is caused by the pathogen's ability to evade host immune defences and establish intracellular infections, intensified by its genomic plasticity and capacity for horizontal gene transfer. To investigate evolutionary adaptations between one older (2019) and four recent (2023)\n                    <jats:italic>E. piscicida<\/jats:italic>\n                    strains from the same geographical locations, we performed comparative genomic analysis of five isolates using high\u2010quality hybrid genome assemblies and compared them with 27 Edwardsiella reference genomes. Pangenome analysis identified distinct novel genomic islands (GIs) specific to the 2023 strains. These GIs (~100\u2009kb in size) shared 85 gene clusters encoding multiple antibiotic resistance genes, phage defence systems, mobilisation genes, and mercury resistance. In addition, they encoded integrases, transposases, and conjugative transfer genes, suggesting they function as integrative and conjugative elements (ICEs), a type of mobile genetic element. Phenotypic characterisation showed the 2023 strains carrying novel GI increased antibiotic resistance, but no significant difference in virulence in Japanese flounder infection trials. These findings highlight the recent genomic diversification of\n                    <jats:italic>E. piscicida<\/jats:italic>\n                    in aquaculture and the importance of monitoring emerging GIs driving antibiotic resistance and environmental persistence.\n                  <\/jats:p>","DOI":"10.1111\/jfd.70035","type":"journal-article","created":{"date-parts":[[2025,8,5]],"date-time":"2025-08-05T10:59:01Z","timestamp":1754391541000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Comparative Genomics of\n                    <i>Edwardsiella piscicida<\/i>\n                    in the Japanese Flounder (\n                    <i>Paralichthys olivaceus<\/i>\n                    ): Discovery and Implications of a Novel Genomic Island"],"prefix":"10.1111","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-6643-6966","authenticated-orcid":false,"given":"Theeyathart","family":"Homsombat","sequence":"first","affiliation":[{"name":"Department of Marine Biosciences Tokyo University of Marine Science and Technology  Minato\u2010ku Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keisuke","family":"Yoshii","sequence":"additional","affiliation":[{"name":"Fisheries Research Division Oita Prefectural Agriculture, Forestry and Fisheries Research Center  Saiki Oita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yutaka","family":"Fukuda","sequence":"additional","affiliation":[{"name":"Fisheries Research Division Oita Prefectural Agriculture, Forestry and Fisheries Research Center  Saiki Oita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8890-8229","authenticated-orcid":false,"given":"Keiichiro","family":"Koiwai","sequence":"additional","affiliation":[{"name":"Department of Marine Biosciences Tokyo University of Marine Science and Technology  Minato\u2010ku Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2355-3121","authenticated-orcid":false,"given":"Ikuo","family":"Hirono","sequence":"additional","affiliation":[{"name":"Department of Marine Biosciences Tokyo University of Marine Science and Technology  Minato\u2010ku Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5102-6831","authenticated-orcid":false,"given":"Hidehiro","family":"Kondo","sequence":"additional","affiliation":[{"name":"Department of Marine Biosciences Tokyo University of Marine Science and Technology  Minato\u2010ku Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,8,5]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2019.00404"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.001485"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022\u20102836(05)80360\u20102"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkad644"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102164\u20109\u201075"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1089\/cmb.2012.0021"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/1574\u20106976.12058"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1099\/mgen.0.000818"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkx343"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.317.7172.1572"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkaa345"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/aem.00991\u201022"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao03281"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00233\u201018"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty560"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky425"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/mmbr.00016\u201010"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/applmicrobiol4040111"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.02065"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro884"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13567\u2010018\u20100613\u2010z"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms10051043"},{"key":"e_1_2_9_26_1","unstructured":"European Committee on Antimicrobial Susceptibility Testing (EUCAST).2023.\u201cBreakpoints Tables for Interpretation of MICs and Zone Diameters (Version 15.0).\u201dhttps:\/\/www.eucast.org\/clinical_breakpoints\/."},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/291067"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2023.739703"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471\u20102164\u201013\u2010347"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/genomea.00927\u201014"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1235"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574\u20106941.2002.tb01020.x"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.64483\u20100"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkad326"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1093\/embo\u2010reports\/kve097"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586\u2010019\u20101894\u20108"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0008094"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics13121204"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.12688\/wellcomeopenres.14826.1"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.01327\u201006"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574\u20106976.2008.00136.x"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2022.738199"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41587\u2010019\u20100072\u20108"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1101\/2019.12.19.882506"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2350"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkab301"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2019.1621648"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2021.2006890"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty191"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12866\u2010021\u201002114\u20104"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.723577"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky1080"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864\u2010022\u201008402\u20101"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gky1123"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.13491"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.4014\/jmb.2301.01008"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms11020515"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty266"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.ARBA\u20100017\u20102017"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msaa015"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.3147\/jsfp.58.175"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv421"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.186072.114"},{"key":"e_1_2_9_64_1","doi-asserted-by":"publisher","DOI":"10.1128\/cmr.00088\u201017"},{"key":"e_1_2_9_65_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.02928"},{"key":"e_1_2_9_66_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.00970\u201017"},{"key":"e_1_2_9_67_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0906412106"},{"key":"e_1_2_9_68_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsle.2005.02.034"},{"key":"e_1_2_9_69_1","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.02060\u201021"},{"key":"e_1_2_9_70_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-981-19-9342-8_1"},{"key":"e_1_2_9_71_1","doi-asserted-by":"publisher","DOI":"10.1128\/cmr.17.1.14\u201056.2004"},{"key":"e_1_2_9_72_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu153"},{"key":"e_1_2_9_73_1","doi-asserted-by":"crossref","unstructured":"Seppey M. M.Manni andE. M.Zdobnov.2019.\u201cBUSCO: Assessing Genome Assembly and Annotation Completeness. Gene Prediction: Methods and Protocols.\u201d 227\u2013245.https:\/\/doi.org\/10.1007\/978\u20101\u20104939\u20109173\u20100_14.","DOI":"10.1007\/978-1-4939-9173-0_14"},{"key":"e_1_2_9_74_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12941\u2010019\u20100317\u2010x"},{"key":"e_1_2_9_75_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40779\u2010021\u201000362\u2010z"},{"key":"e_1_2_9_76_1","doi-asserted-by":"publisher","DOI":"10.1128\/9781555819217.ch26"},{"key":"e_1_2_9_77_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkj014"},{"key":"e_1_2_9_78_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv351"},{"key":"e_1_2_9_79_1","doi-asserted-by":"publisher","DOI":"10.1002\/aah.10169"},{"key":"e_1_2_9_80_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00069\u201011"},{"key":"e_1_2_9_81_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkad313"},{"key":"e_1_2_9_82_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2020.10.029"},{"key":"e_1_2_9_83_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0294236"},{"key":"e_1_2_9_84_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btx713"},{"key":"e_1_2_9_85_1","doi-asserted-by":"publisher","DOI":"10.1099\/mgen.0.000322"},{"key":"e_1_2_9_86_1","doi-asserted-by":"publisher","DOI":"10.1101\/2024.01.25.577194"},{"key":"e_1_2_9_87_1","doi-asserted-by":"publisher","DOI":"10.1002\/0471142727.mb0117s79"},{"key":"e_1_2_9_88_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1000344"},{"key":"e_1_2_9_89_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2011.00203"},{"key":"e_1_2_9_90_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btl369"},{"key":"e_1_2_9_91_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00203\u2010022\u201002757\u20105"},{"key":"e_1_2_9_92_1","doi-asserted-by":"publisher","DOI":"10.3390\/md15060158"},{"key":"e_1_2_9_93_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.01864\u201019"},{"key":"e_1_2_9_94_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev\u2010micro\u2010032521\u2010022006"},{"key":"e_1_2_9_95_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv383"},{"key":"e_1_2_9_96_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.521"},{"key":"e_1_2_9_97_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkad382"},{"key":"e_1_2_9_98_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms252413316"},{"key":"e_1_2_9_99_1","first-page":"67","article-title":"Characteristics of the Fish Pathogen Edwardsiella Isolated From Several Species of Cultured Marine Fish","volume":"8","author":"Yasunaga N.","year":"1982","journal-title":"Bulletin of the Nagasaki Prefectural Institute of Fisheries"},{"key":"e_1_2_9_100_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13258\u2010014\u20100263\u20100"},{"key":"e_1_2_9_101_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2023.739855"},{"key":"e_1_2_9_102_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864\u2010018\u20105366\u20106"},{"key":"e_1_2_9_103_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2021.737127"}],"container-title":["Journal of Fish Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.70035","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jfd.70035","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.70035","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T14:59:48Z","timestamp":1787669988000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jfd.70035"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,5]]},"references-count":102,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2026,2]]}},"alternative-id":["10.1111\/jfd.70035"],"URL":"https:\/\/doi.org\/10.1111\/jfd.70035","archive":["Portico"],"relation":{},"ISSN":["0140-7775","1365-2761"],"issn-type":[{"value":"0140-7775","type":"print"},{"value":"1365-2761","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,5]]},"assertion":[{"value":"2025-03-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-07-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-08-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70035"}}