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From October to November 2023, channel catfishes (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Ictalurus punctatus<\/jats:italic>\n                    <\/jats:styled-content>\n                    ) cultured in Xiaowan reservoir at Dali city, Yunnan province in China, showed gross pathological changes with skin ulcers, white nodules in the liver and heart, histopathological tests revealed the typical granulomas and chronic hepatitis changes in the liver, and the clumps of filamentous bacteria in the heart and spleen. A Gram\u2010positive acid\u2010fast bacterium (CCF_NS01) was isolated from viscera (liver, spleen and kidney); biochemical tests and 16sRNA\u2010GyrB gene concatenated sequences analysis of this isolated microorganism identified it as\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>N. seriolae<\/jats:italic>\n                    <\/jats:styled-content>\n                    . Drug sensitivity testing indicated susceptibility to commonly used antibiotics such as co\u2010trimoxazole, doxycycline, enrofloxacin, florfenicol, amikacin and rifampicin. In isolated CCF_NS01, multiple pathogenic factors corresponding to encoding genes were detected, including\n                    <jats:italic>espG<\/jats:italic>\n                    ,\n                    <jats:italic>mftF<\/jats:italic>\n                    ,\n                    <jats:italic>pcaA<\/jats:italic>\n                    ,\n                    <jats:italic>fadD32<\/jats:italic>\n                    ,\n                    <jats:italic>pks13<\/jats:italic>\n                    ,\n                    <jats:italic>narJ<\/jats:italic>\n                    ,\n                    <jats:italic>feoB<\/jats:italic>\n                    ,\n                    <jats:italic>sodA<\/jats:italic>\n                    ,\n                    <jats:italic>katG<\/jats:italic>\n                    and\n                    <jats:italic>mceF<\/jats:italic>\n                    . 100% mortality with 1\u2009\u00d7\u200910\n                    <jats:sup>8<\/jats:sup>\n                    , 1\u2009\u00d7\u200910\n                    <jats:sup>7<\/jats:sup>\n                    and 1\u2009\u00d7\u200910\n                    <jats:sup>6<\/jats:sup>\n                    \u2009cfu\/mL in a 35\u2010d challenge test period was observed, and the intraperitoneal injection median lethal dose (LD\n                    <jats:sub>50<\/jats:sub>\n                    ) was 1.89\u2009\u00d7\u200910\n                    <jats:sup>4<\/jats:sup>\n                    \u2009cfu\/mL. This is the first isolation report of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>N. seriolae<\/jats:italic>\n                    <\/jats:styled-content>\n                    infection from cultured channel catfish, aiming to warn against the continuous host\u2010range expansion of this pathogen, which is threatening the inland aquaculture industry.\n                  <\/jats:p>","DOI":"10.1111\/jfd.70044","type":"journal-article","created":{"date-parts":[[2025,8,19]],"date-time":"2025-08-19T09:59:54Z","timestamp":1755597594000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["First Case of\n                    <i>Nocardia seriolae<\/i>\n                    Infection in China Cultured Channel Catfish (\n                    <i>Ictalurus punctatus<\/i>\n                    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