{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T13:12:35Z","timestamp":1792069955216,"version":"4.4.0"},"reference-count":25,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T00:00:00Z","timestamp":1757635200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,9,12]],"date-time":"2025-09-12T00:00:00Z","timestamp":1757635200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002850","name":"Fondo Nacional de Desarrollo Cient\u00edfico y Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["1230311"],"award-info":[{"award-number":["1230311"]}],"id":[{"id":"10.13039\/501100002850","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Saprolegnia<\/jats:italic>\n                    sp., a water mold causing significant economic losses in aquaculture, necessitates sustainable alternatives to chemical treatments. This study investigated the resinous exudate of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Psoralea glandulosa<\/jats:italic>\n                    <\/jats:styled-content>\n                    and its derivatives as inhibitors of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Saprolegnia parasitica<\/jats:italic>\n                    <\/jats:styled-content>\n                    and\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>S. australis<\/jats:italic>\n                    <\/jats:styled-content>\n                    . Through in\u00a0vitro assays and in silico molecular docking, the research aimed to determine the inhibitory efficacy and mechanism of action of these natural compounds. The results identified 3\u2010hydroxybakuchiol (2) as the most potent agent. It demonstrated superior minimum inhibitory concentration (MIC) values of 50\u2009\u03bcg\/mL against\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>S. parasitica<\/jats:italic>\n                    <\/jats:styled-content>\n                    and 25\u2009\u03bcg\/mL against\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>S. australis<\/jats:italic>\n                    <\/jats:styled-content>\n                    , surpassing the commercial control, bronopol. Its minimum oomyceticidal concentration (MOC) was also confirmed at low levels (50\u201375\u2009\u03bcg\/mL). Molecular docking analysis revealed that compound 2 has a high binding affinity for two crucial proteins in\n                    <jats:italic>Saprolegnia<\/jats:italic>\n                    : host\u2010targeting protein 1 (Htp\u20101) and plasma membrane ATPase. These findings suggest that compound 2 acts as a potent dual inhibitor, with its free hydroxyl group being critical for its activity. This study positions 3\u2010hydroxybakuchiol as a promising lead compound for developing new, safer, natural\u2010based therapies to control saprolegniasis in the aquaculture industry.\n                  <\/jats:p>","DOI":"10.1111\/jfd.70058","type":"journal-article","created":{"date-parts":[[2025,9,13]],"date-time":"2025-09-13T05:41:11Z","timestamp":1757742071000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Bakuchiol and Related Compounds From\n                    <i>Psoralea glandulosa<\/i>\n                    as Potent Inhibitors of\n                    <i>Saprolegnia<\/i>\n                    sp.: A Combined In\u00a0Vitro and In Silico Study"],"prefix":"10.1111","volume":"49","author":[{"given":"Esperanza","family":"Galvez","sequence":"first","affiliation":[{"name":"Laboratorio de Productos Naturales y S\u00edntesis Org\u00e1nica (LPNSO), Facultad de Ciencias Naturales y Exactas Universidad de Playa Ancha  Valpara\u00edso Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evelyn","family":"Mu\u00f1oz","sequence":"additional","affiliation":[{"name":"Laboratorio de Productos Naturales y S\u00edntesis Org\u00e1nica (LPNSO), Facultad de Ciencias Naturales y Exactas Universidad de Playa Ancha  Valpara\u00edso Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentina","family":"Silva","sequence":"additional","affiliation":[{"name":"Laboratorio de Productos Naturales y S\u00edntesis Org\u00e1nica (LPNSO), Facultad de Ciencias Naturales y Exactas Universidad de Playa Ancha  Valpara\u00edso Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iv\u00e1n","family":"Montenegro","sequence":"additional","affiliation":[{"name":"Escuela de Obstetricia y Puericultura, Facultad de Medicina Universidad de Valpara\u00edso  Vi\u00f1a del Mar Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8977-7032","authenticated-orcid":false,"given":"Alejandro","family":"Madrid","sequence":"additional","affiliation":[{"name":"Laboratorio de Productos Naturales y S\u00edntesis Org\u00e1nica (LPNSO), Facultad de Ciencias Naturales y Exactas Universidad de Playa Ancha  Valpara\u00edso Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,9,12]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/ics.12784"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/ics.12117"},{"key":"e_1_2_9_4_1","first-page":"645","volume-title":"Kibenge","author":"Chong R. 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