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This study investigates the role of pyroptosis, a highly inflammatory programmed cell death, in the immune response of largemouth bass to\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>A. veronii<\/jats:italic>\n                    <\/jats:styled-content>\n                    infection. We characterised key pyroptosis\u2010associated proteins, MsGSDMEa and MsGSDMEb, which harbour two conserved Gasdermin domains and are crucial in mediating pyroptosis. Following\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>A. veronii<\/jats:italic>\n                    <\/jats:styled-content>\n                    infection, largemouth bass exhibited a robust upregulation of inflammatory cytokines (IL\u20101\u03b2, IL\u20108, TNF\u2010\u03b1) and increased lactate dehydrogenase (LDH) activity in serum, indicating extensive cellular damage and inflammation. Quantitative real\u2010time PCR analysis revealed a significant upregulation of pyroptosis\u2010related genes, including NLRP3, ASC, and caspases (Caspase\u20101\/\u22123\/\u22127), in both spleen tissue and GF\u20101 cell line post\u2010infection. Western blot analysis confirmed the cleavage of GSDME, a critical step in pyroptosis execution. These findings underscore the role of pyroptosis in the immune response of largemouth bass to\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>A. veronii<\/jats:italic>\n                    <\/jats:styled-content>\n                    infection and suggest that modulating pyroptosis could be a potential strategy for disease management in freshwater fish species.\n                  <\/jats:p>","DOI":"10.1111\/jfd.70059","type":"journal-article","created":{"date-parts":[[2025,9,23]],"date-time":"2025-09-23T09:05:58Z","timestamp":1758618358000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["<scp>\n                      <i>Aeromonas veronii<\/i>\n                    <\/scp>\n                    Infection Induces Pyroptosis in Largemouth Bass (\n                    <scp>\n                      <i>Micropterus salmoides<\/i>\n                    <\/scp>\n                 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