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Five strains were selected and identified as belonging to the genus\n                    <jats:italic>Weissella<\/jats:italic>\n                    . In\u00a0vitro assays revealed high tolerance to acidic pH (2.0\u20133.0), resistance to bile salts (10%), biofilm formation capacity, hydrophobicity and direct\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Aeromonas hydrophila<\/jats:italic>\n                    <\/jats:styled-content>\n                    inhibition. In in\u00a0vivo tests, strains M13 and M14 promoted significant increases in tambaqui survival (72.2% and 68.7%;\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05) and growth (standard length and eye diameter), as well as water quality (nitrite reduction), highlighting multiple beneficial effects. Innate immunity was activated, with total protein level (I3 and M14) and agglutinating titre (M13 and M14) increases. Intestinal colonisation was confirmed by bacterial counts, and the observed effects were consistent with adhesive and antimicrobial strain properties. The findings demonstrate a synergistic interaction between probiotics, hosts and pathogens, with integrated health, performance and environmental stability benefits. Strains M13 and M14 are, thus, promising alternatives to the use of antibiotics in intensive aquaculture systems, contributing to sustainable and safe tropical fish production strategies.\n                  <\/jats:p>","DOI":"10.1111\/jfd.70061","type":"journal-article","created":{"date-parts":[[2025,9,20]],"date-time":"2025-09-20T07:45:55Z","timestamp":1758354355000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Prospection of Autochthonous Lactic Acid Bacteria Displaying Probiotic Potential to Enhance\n                    <scp>\n                      <i>Colossoma macropomum<\/i>\n                    <\/scp>\n                    Larvae Growth and Immunoprotection Against\n                    <scp>\n                      <i>Aeromonas hydrophila<\/i>\n                    <\/scp>"],"prefix":"10.1111","volume":"49","author":[{"given":"Thayn\u00e1 M.","family":"dos Santos","sequence":"first","affiliation":[{"name":"Graduate Program in Biosciences Federal University of Western Par\u00e1 (UFOPA)  Santar\u00e9m Par\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Layana A. B.","family":"Pereira","sequence":"additional","affiliation":[{"name":"Graduate Program in Aquaculture (PPG\u2010AQUI) Nilton Lins University  Manaus Amazonas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laine P. C.","family":"dos Santos","sequence":"additional","affiliation":[{"name":"Graduate Program in Aquaculture (PPG\u2010AQUI) Nilton Lins University  Manaus Amazonas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrya L.","family":"Lopes","sequence":"additional","affiliation":[{"name":"Graduate Program in Animal Science Federal University of Western Par\u00e1, UFOPA  Santar\u00e9m Par\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larissa A. F.","family":"Sampaio","sequence":"additional","affiliation":[{"name":"Graduate Program in Biosciences Federal University of Western Par\u00e1 (UFOPA)  Santar\u00e9m Par\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Graziella V. G.","family":"de Matos Silva","sequence":"additional","affiliation":[{"name":"Graduate Program in Aquaculture Federal University of Santa Catarina (UFSC)  Florian\u00f3polis Santa Catarina Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana N.","family":"Ferreira","sequence":"additional","affiliation":[{"name":"Graduate Program in Aquaculture (PPG\u2010AQUI) Nilton Lins University  Manaus Amazonas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gleika T. J.","family":"Dos Reis","sequence":"additional","affiliation":[{"name":"Graduate Program in Biosciences Federal University of Western Par\u00e1 (UFOPA)  Santar\u00e9m Par\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonny B.","family":"Teixeira","sequence":"additional","affiliation":[{"name":"Graduate Program in Aquaculture (PPG\u2010AQUI) Nilton Lins University  Manaus Amazonas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raimundo J. T.","family":"Diniz\u2010Neto","sequence":"additional","affiliation":[{"name":"Graduate Program in Aquaculture (PPG\u2010AQUI) Nilton Lins University  Manaus Amazonas Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura G. M.","family":"de Carvalho","sequence":"additional","affiliation":[{"name":"Graduate Program in Animal Science Federal University of Western Par\u00e1, UFOPA  Santar\u00e9m Par\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle Midori Sena","family":"Fugimura","sequence":"additional","affiliation":[{"name":"Graduate Program in Biosciences Federal University of Western Par\u00e1 (UFOPA)  Santar\u00e9m Par\u00e1 Brazil"},{"name":"Graduate Program in Aquaculture (PPG\u2010AQUI) Nilton Lins University  Manaus Amazonas Brazil"},{"name":"Graduate Program in Animal Science Federal University of Western Par\u00e1, UFOPA  Santar\u00e9m Par\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciano Jensen","family":"Vaz","sequence":"additional","affiliation":[{"name":"Graduate Program in Animal Science Federal University of Western Par\u00e1, UFOPA  Santar\u00e9m Par\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia E.","family":"Plaul","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Sciences National University of La Plata (UNLP)  La Plata Buenos Aires Argentina"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5063-9123","authenticated-orcid":false,"given":"Gustavo S.","family":"Claudiano","sequence":"additional","affiliation":[{"name":"Graduate Program in Biosciences Federal University of Western Par\u00e1 (UFOPA)  Santar\u00e9m Par\u00e1 Brazil"},{"name":"Graduate Program in Aquaculture (PPG\u2010AQUI) Nilton Lins University  Manaus Amazonas Brazil"},{"name":"Graduate Program in Animal Science Federal University of Western Par\u00e1, UFOPA  Santar\u00e9m Par\u00e1 Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,9,20]]},"reference":[{"key":"e_1_2_12_2_1","first-page":"24","article-title":"Isolation and Identification of Potential Lactic Acid Bacteria as Probiotics From the Intestines of Repang Fish (Puntioplites waandersi)","volume":"15","author":"Agustina A.","year":"2022","journal-title":"AACL Bioflux"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12602\u2010018\u20109439\u20102"},{"key":"e_1_2_12_4_1","first-page":"1","article-title":"Survival of Probiotic Strains in Acidic and Bile Conditions: A Comparative Study","volume":"234","author":"Ashraf R.","year":"2016","journal-title":"International Journal of Food Microbiology"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.4322\/rbcv.2014.011"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003\u20102697(76)90527\u20103"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2013.11.012"},{"key":"e_1_2_12_8_1","article-title":"Water Quality and Its Relation to Fish Immunity in Aquaculture Systems","volume":"21","author":"Cavalcante D. 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P.2022.\u201cProbi\u00f3tico Aut\u00f3ctone (Enterococcus faecium) na Frequ\u00eancia de Alimenta\u00e7\u00e3o de Larvas de Piau\u00e7uMegaleporinus macrocephalus(Garavello & Britsky 1988) Sobre o Desempenho Produtivo e Resist\u00eancia a Pat\u00f3genos.\u201dUndergraduated Thesis Universidade Federal do Par\u00e1 Bragan\u00e7a\u2010PA 59 pp.https:\/\/bdm.ufpa.br:8443\/jspui\/handle\/prefix\/4378."},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574\u20106976.2006.00017.x"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.1111\/raq.12191"},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2021.737405"},{"key":"e_1_2_12_47_1","doi-asserted-by":"publisher","DOI":"10.1080\/01652176.2016.1172132"}],"container-title":["Journal of Fish Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.70061","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jfd.70061","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.70061","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T07:02:11Z","timestamp":1770966131000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jfd.70061"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,9,20]]},"references-count":46,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2026,3]]}},"alternative-id":["10.1111\/jfd.70061"],"URL":"https:\/\/doi.org\/10.1111\/jfd.70061","archive":["Portico"],"relation":{},"ISSN":["0140-7775","1365-2761"],"issn-type":[{"value":"0140-7775","type":"print"},{"value":"1365-2761","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,9,20]]},"assertion":[{"value":"2025-08-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-11","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-09-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70061"}}