{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T00:53:38Z","timestamp":1788310418221,"version":"build-2803163510"},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T00:00:00Z","timestamp":1763337600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T00:00:00Z","timestamp":1763337600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFC2812105"],"award-info":[{"award-number":["2023YFC2812105"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012428","name":"Central Public-interest Scientific Institution Basal Research Fund, Chinese Academy of Fishery Sciences","doi-asserted-by":"publisher","award":["NO. 2025TD42"],"award-info":[{"award-number":["NO. 2025TD42"]}],"id":[{"id":"10.13039\/501100012428","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2026,4]]},"abstract":"ABSTRACT<\/jats:title>\n \n Turbot (\n \n Scophthalmus maximus<\/jats:italic>\n <\/jats:styled-content>\n ) is an economically important farming fish in China. Turbot circovirus (\n TCV<\/jats:styled-content>\n ), a recently discovered member of the\n Circovirus<\/jats:italic>\n genus within the\n Circoviridae<\/jats:italic>\n family, has emerged as a significant threat to the turbot aquaculture industry in China. This virus is the causative agent of turbot acute hemorrhagic disease (\n TAHD<\/jats:styled-content>\n ), resulting in substantial economic losses in recent years. As a novel pathogen, the development of more accurate and efficient detection methods for\n TCV<\/jats:styled-content>\n remains an urgent need. In this study, the specific probe and primers were designed based on the consensus sequence of the\n cap<\/jats:italic>\n gene of\n TCV<\/jats:styled-content>\n to develop a\n TaqMan<\/jats:styled-content>\n probe\u2010based real\u2010time\n PCR<\/jats:styled-content>\n method for virus detection. The specificity of the assay was validated using turbot reddish body iridovirus (\n TRBIV<\/jats:styled-content>\n ), viral nervous necrosis virus (\n VNNV<\/jats:styled-content>\n ), the\n cap<\/jats:italic>\n gene of European catfish (\n \n Silurus glanis<\/jats:italic>\n <\/jats:styled-content>\n ) circovirus (\n CfCV<\/jats:styled-content>\n ) and European eel (\n \n Anguilla anguilla<\/jats:italic>\n <\/jats:styled-content>\n ) circovirus (\n EeCV<\/jats:styled-content>\n ), and no cross\u2010reaction was observed, indicating the high specificity of the established method. The detection limit of this assay was 10\n 1<\/jats:sup>\n copies per reaction, with an efficiency of 93.7%, a regression squared value of 0.999, and a linear range from 10\n 1<\/jats:sup>\n copies to 10\n 8<\/jats:sup>\n copies of\n TCV DNA<\/jats:styled-content>\n . The assay demonstrated high specificity, sensitivity and reproducibility, with both intra\u2010 and inter\u2010assay coefficients of variation less than 2.0%. Then 390 clinical samples suspected of\n TAHD<\/jats:styled-content>\n collected from 2019 to 2022 were tested using this method, and all the samples tested positive for\n TCV<\/jats:styled-content>\n . In this study, we established a\n TaqMan qPCR<\/jats:styled-content>\n \u2010based method for\n TCV<\/jats:styled-content>\n detection, which could be applied to epidemiological investigations and pathogenic studies of\n TCV<\/jats:styled-content>\n .\n <\/jats:p>","DOI":"10.1111\/jfd.70084","type":"journal-article","created":{"date-parts":[[2025,11,17]],"date-time":"2025-11-17T12:22:11Z","timestamp":1763382131000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Development of a\n TaqMan<\/scp>\n \u2010Based Real\u2010Time\n PCR<\/scp>\n Assay for the Specific Detection of Turbot Circovirus"],"prefix":"10.1111","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-8390-8724","authenticated-orcid":false,"given":"Huilin","family":"Wang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences Qingdao Shandong China"},{"name":"Fisheries College Ocean University of China Qingdao China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xia","family":"Gao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences Qingdao Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"Gao","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences Qingdao Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohan","family":"Shang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences Qingdao Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences Qingdao Shandong China"},{"name":"Laboratory for Marine Fisheries Science and Food Production Processes Qingdao Marine Science and Technology Center Qingdao Shandong China"},{"name":"Tutbot Branch China Aquatic Products Processing and Marketing Alliance Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,17]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0378\u20101135(99)00004\u20108"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00705\u2010005\u20100639\u20101"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1556\/avet.51.2003.4.13"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.virusres.2011.11.021"},{"issue":"1","key":"e_1_2_9_6_1","first-page":"44","article-title":"Isolation of Circovirus From Lesions of Pigs With Postweaning Multisystemic Wasting Syndrome","volume":"39","author":"Ellis J.","year":"1998","journal-title":"Canadian Veterinary 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