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Microorganisms are entrapped in extruded DNA fibres co\u2010localised with antimicrobial peptides and eventually killed. However, as a cell death mechanism, its strict regulation is essential as excessive formation of ETs may cause detrimental effects to the host by contributing to disease pathophysiology. Here, we investigated the ability of freeze\u2010dried\n                    <jats:italic>Lactiplantibacillus plantarum<\/jats:italic>\n                    (FD\u2010LAB), previously reported to enhance shrimp immunity against pathogenic infections, to regulate ETosis. In an ex\u00a0vivo setup, gill cells and circulating hemocytes were pre\u2010exposed to FD\u2010LAB and were then stimulated with\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Vibrio parahaemolyticus<\/jats:italic>\n                    <\/jats:styled-content>\n                    . Results showed that\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>V. parahaemolyticus<\/jats:italic>\n                    <\/jats:styled-content>\n                    alone induced ETosis in both gill cells and circulating hemocytes, while FD\u2010LAB alone did not. However, when cells were pre\u2010exposed to FD\u2010LAB prior to stimulation with\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>V. parahaemolyticus<\/jats:italic>\n                    <\/jats:styled-content>\n                    , no ETosis occurred. Similarly, changes in reactive oxygen species (ROS) production coincided with the formation of ETs, thus signifying that FD\u2010LAB may regulate ETosis in shrimp gill cells and circulating hemocytes possibly by dampening ROS production. These results present a novel means to regulate ETosis and indicate that FD\u2010LAB may enhance shrimp immunity while also acting on immune regulation.\n                  <\/jats:p>","DOI":"10.1111\/jfd.70092","type":"journal-article","created":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T08:11:12Z","timestamp":1764317472000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Freeze\u2010Dried\n                    <i>Lactiplantibacillus plantarum<\/i>\n                    Regulates Formation of Extracellular Traps in\n                    <i>Penaeus vannamei<\/i>\n                    Ex\u00a0Vivo"],"prefix":"10.1111","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2753-6385","authenticated-orcid":false,"given":"John Paul Matthew Domingo","family":"Guzman","sequence":"first","affiliation":[{"name":"Laboratory of Genome Science Tokyo University of Marine Science and Technology  Tokyo Japan"},{"name":"Environment and Biotechnology Division Industrial Technology Development Institute Department of Science and Technology  Taguig City Philippines"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudio","family":"Gargano","sequence":"additional","affiliation":[{"name":"Laboratory of Genome Science Tokyo University of Marine Science and Technology  Tokyo Japan"},{"name":"Department of Biological, Chemical and Pharmaceutical Sciences and Technologies Universita di Palermo  Palermo Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mikio","family":"Aoki","sequence":"additional","affiliation":[{"name":"Sumitomo Chemicals Co. Ltd.  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroshi","family":"Kuwahara","sequence":"additional","affiliation":[{"name":"Sumitomo Chemicals Co. Ltd.  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8890-8229","authenticated-orcid":false,"given":"Keiichiro","family":"Koiwai","sequence":"additional","affiliation":[{"name":"Laboratory of Genome Science Tokyo University of Marine Science and Technology  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5102-6831","authenticated-orcid":false,"given":"Hidehiro","family":"Kondo","sequence":"additional","affiliation":[{"name":"Laboratory of Genome Science Tokyo University of Marine Science and Technology  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2355-3121","authenticated-orcid":false,"given":"Ikuo","family":"Hirono","sequence":"additional","affiliation":[{"name":"Laboratory of Genome Science Tokyo University of Marine Science and Technology  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,11,28]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1900156"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/BIOLOGY9080234"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2023.108992"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2635"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/S12906\u2010016\u20101375\u20107\/FIGURES\/6"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41581\u2010019\u20100163\u20102"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.02084\u201018"},{"key":"e_1_2_11_9_1","unstructured":"Fitzpatrick M.2021.\u201cThe Open Lab Book Release 1.0.\u201d"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/929743"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2025.110950"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2024.742092"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2024.110091"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/J.FSI.2016.03.039"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2018.04.036"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/genomeA.00221\u201014"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1182\/BLOOD\u20102015\u201004\u2010641142"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2013.06.014"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.29245\/2578-3009\/2018\/5.1161"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.3856\/VOL49\u2010ISSUE1\u2010FULLTEXT\u20102514"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2017.105"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1371\/JOURNAL.PONE.0020960"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms5627"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.3390\/MICROORGANISMS9102027"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/ANTIOX11091683\/S1"},{"key":"e_1_2_11_26_1","doi-asserted-by":"crossref","unstructured":"Sofia P. 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