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Among these, four isolates demonstrated strong inhibitory effects, producing inhibition zones of 16\u201320\u2009mm in agar well diffusion assays. These four LAB isolates were reported to cause no shrimp mortality during the feeding trials. Notably, shrimp fed with the isolate CG02 for 4\u2009weeks showed a significantly improved survival (\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.05) after the immersion challenge with\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>V. parahaemolyticus<\/jats:italic>\n                    <\/jats:styled-content>\n                    strain pVPA3\u20101. Based on 16S rDNA sequencing, the isolate CG02 was identified as\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Lactobacillus plantarum<\/jats:italic>\n                    <\/jats:styled-content>\n                    . To improve its viability, the isolate CG02 was encapsulated into spray\u2010dried microcapsules. The resulting probiotic powder exhibited high cell viability, with counts of 9\u2009\u00b1\u20091.45\u2009\u00d7\u200910\n                    <jats:sup>8<\/jats:sup>\n                    \u2009CFU\u2009g\n                    <jats:sup>\u22121<\/jats:sup>\n                    after 4\u2009months at room temperature, and 4.11\u2009\u00b1\u20090.38\u2009\u00d7\u200910\n                    <jats:sup>8<\/jats:sup>\n                    \u2009CFU\u2009g\n                    <jats:sup>\u22121<\/jats:sup>\n                    after 8\u2009months at 4\u00b0C. When incorporated into shrimp feed, the CG02 powder did not cause any shrimp mortality. Moreover, it provided a relative percent survival (RPS) of 34.78% compared to the control group following the immersion challenge with\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>V. parahaemolyticus<\/jats:italic>\n                    <\/jats:styled-content>\n                    . These results highlight the potential of spray\u2010dried\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>L. plantarum<\/jats:italic>\n                    <\/jats:styled-content>\n                    CG02 as a probiotic feed additive for enhancing shrimp health and supporting sustainable aquaculture practices.\n                  <\/jats:p>","DOI":"10.1111\/jfd.70101","type":"journal-article","created":{"date-parts":[[2025,12,9]],"date-time":"2025-12-09T12:32:58Z","timestamp":1765283578000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Efficacy of\n                    <i>Lactobacillus plantarum CG02<\/i>\n                    Against\n                    <i>Vibrio parahaemolyticus<\/i>\n                    <scp>pVPA3<\/scp>\n                    \u20101 Infection in Whiteleg Shrimp (\n                    <i>Penaeus vannamei<\/i>\n                    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