{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T16:00:13Z","timestamp":1781193613486,"version":"3.54.1"},"reference-count":42,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T00:00:00Z","timestamp":1765843200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2025,12,16]],"date-time":"2025-12-16T00:00:00Z","timestamp":1765843200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100009037","name":"Science and Technology Research Partnership for Sustainable Development","doi-asserted-by":"publisher","award":["SATREPS JPMJSA1806"],"award-info":[{"award-number":["SATREPS JPMJSA1806"]}],"id":[{"id":"10.13039\/501100009037","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The\n                    <jats:italic>Megalocytivirus pagrus1<\/jats:italic>\n                    causes serious disease in aquaculture and is classified into three clades: infectious spleen and kidney necrosis virus (ISKNV), turbot reddish body iridovirus (TRBIV), and red sea bream iridovirus (RSIV), further classified as genotype I or II. Several recent studies have reported RSIV strains designated as mixed genotype I\/II because they were assigned to different subtypes depending on the gene used for phylogenetic analysis. In 2024, three RSIV samples were collected in Japan, one of which (GT1\u2010Oita2024) was PCR\u2010positive but tested negative using an immune fluorescent antibody test (IFAT) with M10 antibodies. Thus, we performed whole genome sequencing and phylogenetic analysis of the IFAT\u2010negative sample and the two other RSIV samples (GT2\u2010Oita2024 and Ehime2024). In GT1\u2010Oita2024,\n                    <jats:italic>LEGFD,<\/jats:italic>\n                    a known antigen of the M10 antibody, was found to harbour several mutations. Based on average nucleotide identity, GT2\u2010Oita2024 and Ehime2024 were classified as genotype II, showing high homology with KagYT\u201096 and RIE12\u20101. GT1\u2010Oita2024 was classified as the intermediate type, showing high homology with SBIV\u2010VP13 and 17SbTy. GT1\u2010Oita2024 also contained regions showing homology with genotype I, genotype II and TRBIV. This is the first detection of this genotype in Japan. Further monitoring is warranted to determine the extent of its spread.\n                  <\/jats:p>","DOI":"10.1111\/jfd.70108","type":"journal-article","created":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T06:24:50Z","timestamp":1765952690000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Genomic Characterisation of Red Sea Bream Iridovirus (RSIV) Samples Collected in Japan in 2024 Revealed Genotype II and the First Detection of the Intermediate Type in Japan"],"prefix":"10.1111","volume":"49","author":[{"given":"Kazuma","family":"Yoshimura","sequence":"first","affiliation":[{"name":"Tokyo University of Marine Science and Technology  Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miho","family":"Furukawa","sequence":"additional","affiliation":[{"name":"Tokyo University of Marine Science and Technology  Tokyo 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