{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T14:55:14Z","timestamp":1788274514149,"version":"build-2803163510"},"reference-count":38,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T00:00:00Z","timestamp":1767830400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T00:00:00Z","timestamp":1767830400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000202","name":"U.S. Fish and Wildlife Service","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000202","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008745","name":"Alabama Agricultural Experiment Station","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008745","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    A captive\u2010reared cohort of Laurel Dace,\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Chrosomus saylori<\/jats:italic>\n                    <\/jats:styled-content>\n                    (Skelton 2001) (Cypriniformes: Leuciscidae), a federally\u2010endangered species endemic to several streams in eastern Tennessee's Cumberland Plateau, exhibited signs of morbidity and trickling mortalities in a recirculating aquaculture system. Ziehl\u2010Neelsen (ZN) staining revealed acid\u2010fast rod\u2010shaped bacteria within necrotic and intracellular lesions affecting the eye, swim bladder, spleen, and ovary. Histopathology showed granulomatous inflammation characterised by macrophages, multinucleated giant cells, and eosinophilic exudates. The eye, swim bladder, and ovary were particularly impacted: the choroid plexus of the eye displayed haemorrhages and dense cellular infiltrates; the swim bladder exhibited granulomatous inflammation with extensive fibrosis; the ovary contained caseous granulomas; and the choroid plexus of the eye displayed haemorrhages and dense cellular infiltrates. PCR amplification of the\n                    <jats:italic>hsp65<\/jats:italic>\n                    gene (316\u2009bp) from formalin\u2010fixed and paraffin\u2010embedded lesioned tissues was identical (100% similar; 424\u2009bp fragment) to that of the type strain of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Mycobacterium gordonae<\/jats:italic>\n                    <\/jats:styled-content>\n                    Bojalil et\u00a0al. 1962 (AF547840). This is the first report of an infection by\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>M. gordonae<\/jats:italic>\n                    <\/jats:styled-content>\n                    in Laurel Dace; the first record of this bacterium causing a lesion in the swim bladder, eye, or ovary; and the first record of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>M. gordonae<\/jats:italic>\n                    <\/jats:styled-content>\n                    from a freshwater fish in North America.\n                  <\/jats:p>","DOI":"10.1111\/jfd.70116","type":"journal-article","created":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T11:41:42Z","timestamp":1767872502000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["<i>Mycobacterium gordonae<\/i>\n                    (Actinomycetota: Mycobacteriales: Mycobacteriaceae) Infecting Captive\u2010Reared Laurel Dace,\n                    <i>Chrosomus saylori<\/i>\n                    (Cypriniformes: Leuciscidae) in North America"],"prefix":"10.1111","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8476-0255","authenticated-orcid":false,"given":"Daniel","family":"Gordillo\u2010Gonz\u00e1lez","sequence":"first","affiliation":[{"name":"Southeastern Cooperative Fish Parasite and Disease Laboratory, School of Fisheries, Aquaculture, and Aquatic Sciences, College of Agriculture Auburn University  Auburn Alabama USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5769-5718","authenticated-orcid":false,"given":"Steven P.","family":"Ksepka","sequence":"additional","affiliation":[{"name":"Southeastern Cooperative Fish Parasite and Disease Laboratory, School of Fisheries, Aquaculture, and Aquatic Sciences, College of Agriculture Auburn University  Auburn Alabama USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haley R.","family":"Dutton","sequence":"additional","affiliation":[{"name":"Southeastern Cooperative Fish Parasite and Disease Laboratory, School of Fisheries, Aquaculture, and Aquatic Sciences, College of Agriculture Auburn University  Auburn Alabama USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamila","family":"Cajiao\u2010Mora","sequence":"additional","affiliation":[{"name":"Southeastern Cooperative Fish Parasite and Disease Laboratory, School of Fisheries, Aquaculture, and Aquatic Sciences, College of Agriculture Auburn University  Auburn Alabama USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John H.","family":"Brule","sequence":"additional","affiliation":[{"name":"Southeastern Cooperative Fish Parasite and Disease Laboratory, School of Fisheries, Aquaculture, and Aquatic Sciences, College of Agriculture Auburn University  Auburn Alabama USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernard R.","family":"Kuhajda","sequence":"additional","affiliation":[{"name":"Tennessee Aquarium Conservation Institute  Chattanooga Tennessee USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna L.","family":"George","sequence":"additional","affiliation":[{"name":"Tennessee Aquarium Conservation Institute  Chattanooga Tennessee USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen A.","family":"Bullard","sequence":"additional","affiliation":[{"name":"Southeastern Cooperative Fish Parasite and Disease Laboratory, School of Fisheries, Aquaculture, and Aquatic Sciences, College of Agriculture Auburn University  Auburn Alabama USA"},{"name":"Southeastern Cooperative Fish Genetics Laboratory, School of Fisheries, Aquaculture, and Aquatic Sciences, College of Agriculture Auburn University  Auburn Alabama USA"},{"name":"Department of Zoology, School for Environmental Sciences and Development North\u2010West University  Potchefstroom South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,8]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2761.2006.00729.x"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1099\/00221287\u201028\u20102\u2010333"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/sysbio\/syw037"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/ar.10103"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.63222-0"},{"key":"e_1_2_10_7_1","volume-title":"Catalog of Fishes: Genera, Species","author":"Eschmeyer W. 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