{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T15:11:54Z","timestamp":1780585914740,"version":"3.54.1"},"reference-count":68,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T00:00:00Z","timestamp":1769731200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T00:00:00Z","timestamp":1769731200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100006752","name":"U.S. Army Corps of Engineers","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006752","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000203","name":"U.S. Geological Survey","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000203","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000202","name":"U.S. Fish and Wildlife Service","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000202","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009612","name":"Oregon State University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100009612","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100018037","name":"Wildlife Management Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100018037","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2026,7]]},"abstract":"ABSTRACT<\/jats:title>\n \n Adult Chinook salmon (\n \n Oncorhynchus tshawytscha<\/jats:italic>\n <\/jats:styled-content>\n ) trap\u2010and\u2010haul operations exist in rivers across the Pacific Northwest with the objective of re\u2010establishing salmon populations above impoundments. These efforts are complicated by high prespawn mortality (PSM), which can hinder restoration by reducing reproductive success. Recent improvements at trapping facilities in the Willamette River Basin, Oregon, USA, may reduce PSM by streamlining trap\u2010and\u2010haul operations to reduce handling stress. However, studies suggest that PSM is also related to factors associated with haul operations, including fish density and exposure to external stressors such as pathogens. Environmental DNA (eDNA) can help detect pathogens in confined systems with high fish\u2010to\u2010water ratios. In this work, pathogen eDNA samples were collected from anaesthesia and transport tank waters at a trap\u2010and\u2010haul facility in 2017 and 2021 and analysed to assess pathogen presence. Pathogen DNA was detected in both tanks prior to fish addition, including\n \n Aeromonas salmonicida<\/jats:italic>\n <\/jats:styled-content>\n ,\n \n Renibacterium salmoninarum<\/jats:italic>\n <\/jats:styled-content>\n ,\n \n Salmincola californiensis<\/jats:italic>\n <\/jats:styled-content>\n , and\n Ceratonova shasta.<\/jats:italic>\n After fish entered the anaesthesia tank, average pathogen concentrations increased in magnitude for\n \n A. salmonicida<\/jats:italic>\n <\/jats:styled-content>\n (73%),\n \n S. californiensis<\/jats:italic>\n <\/jats:styled-content>\n (3165%),\n \n C. shasta<\/jats:italic>\n <\/jats:styled-content>\n (1619%), and decreased for\n \n R. salmoninarum<\/jats:italic>\n <\/jats:styled-content>\n (\u221257%). Results suggest shedding of horizontally transmitted pathogens, particularly\n \n A. salmonicida<\/jats:italic>\n <\/jats:styled-content>\n , during transport.\n <\/jats:p>","DOI":"10.1111\/jfd.70123","type":"journal-article","created":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T12:32:56Z","timestamp":1769776376000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Quantifying Pathogen\n eDNA<\/scp>\n at a Trap\u2010and\u2010Haul Facility for Adult Chinook Salmon (\n Oncorhynchus tshawytscha<\/i>\n )"],"prefix":"10.1111","volume":"49","author":[{"given":"Katherine C.","family":"Carey","sequence":"first","affiliation":[{"name":"Department of Fisheries, Wildlife, and Conservation Sciences Oregon State University Corvallis Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael L.","family":"Kent","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences Oregon State University Corvallis Oregon USA"},{"name":"Department of Microbiology Oregon State University Corvallis Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren","family":"Norris","sequence":"additional","affiliation":[{"name":"Department of Antibody Purification, Immunology Consultants Laboratory Portland Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9970-7628","authenticated-orcid":false,"given":"Justin L.","family":"Sanders","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences Oregon State University Corvallis Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carl B.","family":"Schreck","sequence":"additional","affiliation":[{"name":"Department of Fisheries, Wildlife, and Conservation Sciences Oregon State University Corvallis Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire E.","family":"Couch","sequence":"additional","affiliation":[{"name":"Department of Fisheries, Wildlife, and Conservation Sciences Oregon State University Corvallis Oregon USA"},{"name":"U.S. Geological Survey Western Fisheries Research Center Corvallis Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corbin J.","family":"Schuster","sequence":"additional","affiliation":[{"name":"Department of Microbiology Oregon State University Corvallis Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7709-8590","authenticated-orcid":false,"given":"James T.","family":"Peterson","sequence":"additional","affiliation":[{"name":"Department of Fisheries, Wildlife, and Conservation Sciences Oregon State University Corvallis Oregon USA"},{"name":"U.S. Geological Survey, Oregon Cooperative Fish and Wildlife Research Unit, Department of Fisheries, Wildlife, and Conservation Sciences Oregon State University Corvallis Oregon USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,1,30]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao063151"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.19228"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.2307\/3276714"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pt.2022.12.010"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocon.2006.01.019"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1080\/00028487.2015.1073621"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.2307\/3275341"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253\u2010014\u20105531\u2010z"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tree.2014.05.012"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1080\/00028487.2017.1377110"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102761.2010.01178.x"},{"key":"e_1_2_11_13_1","unstructured":"Bruno D. W. A. L. S.Munro andE. A.Needham.1986.\u201cGill Lesions Caused byAeromonas salmonicidain Sea\u2010Reared Atlantic Salmon Salmo salar L. International Council for the Exploration of the Sea Mariculture Committee CM 1986\/F 6.\u201d"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/rra.1565"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/nafm.11035"},{"key":"e_1_2_11_16_1","volume-title":"Design of Fishways and Other Fish Facilities","author":"Clay C. 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L.Kent.2011.\u201cMigration Behavior and Spawning Success of Spring Chinook Salmon in Fall Creek and the North Fork Middle Fork Willamette River: Relationships Among Fate Fish Condition and Environmental Factors 2010 (Technical Report 2011\u20108) Moscow Idaho.\u201d"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao038075"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1080\/00028487.2015.1052558"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1017\/s0031182020001109"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/aah.10206"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao012091"},{"key":"e_1_2_11_43_1","unstructured":"National Marine Fisheries Service (NMFS).2008.\u201cEndangered Species Act Section\u00a07(a)(2) Consultation Biological Opinion and Magnuson\u2010Stevens Fishery Conservation and I Act Essential Fish Habitat Consultation Consultation on the Willamette River Basin 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