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Amid rising antibiotic resistance, bacteriophage therapy offers a sustainable alternative. This study aimed to isolate, characterise, and evaluate the therapeutic potential of a lytic phage targeting\n                    <jats:italic>L. petauri<\/jats:italic>\n                    . Phage Lp ACM616_1 was isolated from aquaculture effluents in T\u00fcrkiye using\n                    <jats:italic>L. petauri<\/jats:italic>\n                    as the host. Whole\u2010genome analysis and transmission electron microscopy were used to identify it as a member of the Caudoviricetes class. It has an icosahedral head (~31\u2009nm) and a long tail (~107\u2009nm). Host range testing showed specificity to 10 of 11\n                    <jats:italic>L. petauri<\/jats:italic>\n                    strains, with no activity against other pathogens. Lp ACM616_1 exhibited robust stability (70%\u201390% viability at \u221220\u00b0C to 50\u00b0C; &gt;\u200975% at pH 4\u201310), rapid adsorption (&gt;\u200985% in 30\u2009min), a 10\u201320\u2009min latent period, and high burst size. In\u00a0vitro, it suppressed bacterial growth at low MOIs and showed no cytotoxicity in EPC cells. Genomic characterisation revealed a 23,157\u2009bp double\u2010stranded DNA genome with 36.3% GC content and 40 predicted coding sequences, supporting its classification as a novel species within the genus\n                    <jats:italic>Chertseyvirus<\/jats:italic>\n                    lacking virulence and antimicrobial resistance genes. In rainbow trout challenge trials, intraperitoneal phage injection of the phage post\u2010infection significantly improved survival to 77% compared to 40% in the untreated control group. In contrast, oral delivery via feed was found to be ineffective, yielding a survival rate of 42%. This first\n                    <jats:italic>L. petauri<\/jats:italic>\n                    \u2010specific phage demonstrates promising biocontrol potential, advocating for cocktails and encapsulation to enhance aquaculture applications.\n                  <\/jats:p>","DOI":"10.1111\/jfd.70124","type":"journal-article","created":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T09:33:39Z","timestamp":1768469619000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Genomic Characterisation and In\u00a0Vivo Therapeutic Evaluation of the Novel Phage Lp ACM616_1 Targeting\n                    <i>Lactococcus petauri<\/i>"],"prefix":"10.1111","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3475-521X","authenticated-orcid":false,"given":"Ilhan","family":"Altinok","sequence":"first","affiliation":[{"name":"Department of Fisheries Technology Engineering, Faculty of Marine Sciences Karadeniz Technical 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