{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T15:08:01Z","timestamp":1780585681851,"version":"3.54.1"},"reference-count":37,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T00:00:00Z","timestamp":1769990400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T00:00:00Z","timestamp":1769990400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Chemokines are essential for vertebrate immune regulation, and teleosts possess diverse CXCL8 lineages. However, studies on CXCL8 in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C. auratus<\/jats:italic>\n                    <\/jats:styled-content>\n                    remain limited. In the present study, four CXCL8 homologues (\n                    <jats:italic>CaCXCL8\u20101<\/jats:italic>\n                    to\n                    <jats:italic>\u20104<\/jats:italic>\n                    ) were successfully cloned and identified from\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>C. auratus<\/jats:italic>\n                    <\/jats:styled-content>\n                    , and their expression patterns were analysed in the tissues of healthy and those naturally infected with\n                    <jats:italic>H. doneci<\/jats:italic>\n                    . Bioinformatic analyses revealed that all homologues possess the conserved structural features of CXC chemokines. However, the divergence was observed in the N\u2010terminal ELR motif: CaCXCL8\u20101 and CaCXCL8\u20102 harboured a QLR motif, whereas CaCXCL8\u20103 and CaCXCL8\u20104 possessed a DPR motif. Phylogenetic analysis classified CaCXCL8\u20101 and CaCXCL8\u20102 into the CXCL8_L2 lineage, and CaCXCL8\u20103 and CaCXCL8\u20104 into the teleost\u2010specific CXCL8_L1b lineage. Tertiary structure predictions indicated that CaCXCL8\u20101, \u22123, and \u22124 conserve key receptor\u2010binding sites, whereas CaCXCL8\u20102 retained only GAG\u2010binding sites, suggesting potential functional divergence. Under healthy conditions, the four homologues were all constitutively expressed but displayed divergent basal profiles. Following\n                    <jats:italic>H. doneci<\/jats:italic>\n                    infection, they exhibited distinct tissue\u2010specific expression patterns:\n                    <jats:italic>CaCXCL8\u20101<\/jats:italic>\n                    was significantly upregulated in the gills;\n                    <jats:italic>CaCXCL8\u20102<\/jats:italic>\n                    was specifically induced in the spleen;\n                    <jats:italic>CaCXCL8\u20103<\/jats:italic>\n                    was markedly upregulated in both the gills and head kidney; and\n                    <jats:italic>CaCXCL8\u20104<\/jats:italic>\n                    was upregulated in the liver and head kidney but downregulated in the spleen. These divergent expression patterns suggest functional specialisation among the CaCXCL8 homologues, with different paralogs potentially coordinating localised versus systemic immune responses. Our findings provide evidence for the functional diversification of the CXCL8 chemokine in teleosts and contribute to understanding the evolution of complex immune systems in vertebrates.\n                  <\/jats:p>","DOI":"10.1111\/jfd.70135","type":"journal-article","created":{"date-parts":[[2026,2,2]],"date-time":"2026-02-02T10:35:42Z","timestamp":1770028542000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Study on the Diversity of the\n                    <scp>CXCL8<\/scp>\n                    Family in\n                    <i>Carassius auratus<\/i>\n                    : Homologue Characteristics and Tissue\u2010Specific Immune Responses Under\n                    <i>H. doneci<\/i>\n                    Infection"],"prefix":"10.1111","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9878-9051","authenticated-orcid":false,"given":"Jin\u2010ye","family":"Zhang","sequence":"first","affiliation":[{"name":"Chongqing Key Laboratory of Conservation and Utilization of Freshwater Fishes Chongqing Normal University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong\u2010yan","family":"Liu","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Conservation and Utilization of Freshwater Fishes Chongqing Normal University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue\u2010ru","family":"Wang","sequence":"additional","affiliation":[{"name":"Animal Biology Key Laboratory of Chongqing Education Commission Chongqing Normal University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan\u2010jun","family":"Zhao","sequence":"additional","affiliation":[{"name":"Chongqing Key Laboratory of Conservation and Utilization of Freshwater Fishes Chongqing Normal University  Chongqing 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