{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T15:11:33Z","timestamp":1780585893141,"version":"3.54.1"},"reference-count":66,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T00:00:00Z","timestamp":1770163200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T00:00:00Z","timestamp":1770163200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Edwardsiella anguillarum<\/jats:italic>\n                    is a gram\u2010negative bacterium, synonymous with previously described atypical, fish\u2010pathogenic\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Edwardsiella tarda<\/jats:italic>\n                    <\/jats:styled-content>\n                    . Originally described from eels in 2015,\n                    <jats:italic>E.\u2009anguillarum<\/jats:italic>\n                    is an important global fish pathogen, particularly in tilapia. This study describes intraspecific phenotypic and genotypic variability among 17\n                    <jats:italic>E.\u2009anguillarum<\/jats:italic>\n                    isolates from non\u2010anguillid fish hosts and varied geographic origins. Isolates demonstrated similar biochemical characteristics, with slight variation in motility and hydrogen sulfide production. Genomic relatedness among isolates was analysed with repetitive extragenic palindromic sequence\u2010based PCR (rep\u2010PCR) and multilocus sequence analysis (MLSA). Rep\u2010PCR with the ERIC II primer set revealed two distinct genetic clusters, while amplification strategies utilising the GTG\n                    <jats:sub>5<\/jats:sub>\n                    , BOX or ERIC I&amp;II primer sets yielded more uniform profiles. While rep\u2010PCR deemed the isolates largely clonal, MLSA schemes using reference genes from published\n                    <jats:italic>Edwardsiella<\/jats:italic>\n                    MLSA studies revealed\n                    <jats:italic>E.\u2009anguillarum<\/jats:italic>\n                    isolates formed five discrete phylogroups. A unique, ~91.5\u2009kB plasmid was identified in Costa Rican and Colombian isolates, associated with conjugative and transposable elements, plasmid mobilisation, and adhesion; however, no plasmid mediated antibiotic resistance genes were identified. This study provides insight into genetic diversity among\n                    <jats:italic>E.\u2009anguillarum<\/jats:italic>\n                    isolates from different hosts and geographic regions, identifying an optimal MLSA scheme from previous reports applicable to\n                    <jats:italic>E.\u2009anguillarum<\/jats:italic>\n                    isolates.\n                  <\/jats:p>","DOI":"10.1111\/jfd.70136","type":"journal-article","created":{"date-parts":[[2026,2,5]],"date-time":"2026-02-05T04:30:37Z","timestamp":1770265837000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Intraspecific Variation of\n                    <i>Edwardsiella anguillarum<\/i>\n                    From Non\u2010Anguillid Fish From Varied Geographic Origins"],"prefix":"10.1111","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5695-6875","authenticated-orcid":false,"given":"Abigail 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University of California Davis  Davis California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8304-4807","authenticated-orcid":false,"given":"Cynthia B.","family":"Stine","sequence":"additional","affiliation":[{"name":"Food and Drug Administration Center for Veterinary Medicine, Office of Applied Science  Laurel Maryland USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cynthia C.","family":"Ware","sequence":"additional","affiliation":[{"name":"Department of Pathobiology and Population Medicine, College of Veterinary Medicine Mississippi State University  Stoneville Mississippi USA"},{"name":"Thad Cochran National Warmwater Aquaculture Center, Delta Research and Extension Center Mississippi State University  Stoneville Mississippi 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