{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T20:27:21Z","timestamp":1783110441283,"version":"3.54.6"},"reference-count":35,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,2,22]],"date-time":"2026-02-22T00:00:00Z","timestamp":1771718400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,22]],"date-time":"2026-02-22T00:00:00Z","timestamp":1771718400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["25ZR1402190"],"award-info":[{"award-number":["25ZR1402190"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32130109"],"award-info":[{"award-number":["32130109"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Hepcidin is a cysteine\u2010rich peptide that functions both as an antimicrobial peptide and a central regulator of iron homeostasis in vertebrates. Structurally, it consists of an N\u2010terminal signal peptide, a propeptide region and a C\u2010terminal bioactive mature peptide. The canonical mature hepcidin contains eight conserved cysteine residues that form four disulfide bonds, stabilising a characteristic \u03b2\u2010sheet hairpin\u2010like structure. In the Antarctic toothfish (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Dissostichus mawsoni<\/jats:italic>\n                    <\/jats:styled-content>\n                    ), which inhabits extremely cold marine environments, a unique hepcidin variant was identified possessing only four cysteine residues, designated dm_4cyshep. To evaluate the antimicrobial function of the dm_4cyshep peptide, the minimum inhibitory concentration (MIC) assays and flow cytometry analyses were performed. The peptide demonstrated binding affinity and bactericidal activity against a range of Gram\u2010positive and Gram\u2010negative bacteria, with distinct MIC values observed for different strains. Furthermore, overexpression of dm_4cyshep in a hepcidin\u2010deficient zebrafish model markedly reduced systemic iron accumulation, indicating a conserved role in iron regulation. These results demonstrate that despite the reduced number of cysteine residues, dm_4cyshep retains both antimicrobial and iron\u2010modulatory functions, underscoring its potential as an immune effector molecule. This study enhances the understanding of the structural and functional diversity of fish hepcidins and identifies a promising candidate for improving disease resistance in aquaculture.\n                  <\/jats:p>","DOI":"10.1111\/jfd.70142","type":"journal-article","created":{"date-parts":[[2026,2,22]],"date-time":"2026-02-22T12:40:10Z","timestamp":1771764010000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Dual Function Characterisation of the 4\u2010Cysteine Hepcidin in the Antarctic Toothfish\n                    <scp>\n                      <i>Dissostichus mawsoni<\/i>\n                    <\/scp>"],"prefix":"10.1111","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7798-6045","authenticated-orcid":false,"given":"Mingli","family":"Liu","sequence":"first","affiliation":[{"name":"Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education Shanghai Ocean University  Shanghai China"},{"name":"International Research Centre for Marine Biosciences, Ministry of Science and Technology Shanghai Ocean University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruiqin","family":"Hu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education Shanghai Ocean University  Shanghai China"},{"name":"International Research Centre for Marine Biosciences, Ministry of Science and Technology Shanghai Ocean University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5845-0018","authenticated-orcid":false,"given":"Wanying","family":"Zhai","sequence":"additional","affiliation":[{"name":"Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education Shanghai Ocean University  Shanghai China"},{"name":"International Research Centre for Marine 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China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peng","family":"Hu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education Shanghai Ocean University  Shanghai China"},{"name":"International Research Centre for Marine Biosciences, Ministry of Science and Technology Shanghai Ocean University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianghua","family":"Xu","sequence":"additional","affiliation":[{"name":"Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education Shanghai Ocean University  Shanghai China"},{"name":"International Research Centre for Marine Biosciences, Ministry of Science and Technology Shanghai Ocean University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liangbiao","family":"Chen","sequence":"additional","affiliation":[{"name":"Key Laboratory of 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