{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T15:10:13Z","timestamp":1780585813403,"version":"3.54.1"},"reference-count":59,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T00:00:00Z","timestamp":1771372800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T00:00:00Z","timestamp":1771372800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100020884","name":"Agencia Nacional de Investigaci\u00f3n y Desarrollo","doi-asserted-by":"publisher","award":["ANID INCAR\u00b2 CIA250009"],"award-info":[{"award-number":["ANID INCAR\u00b2 CIA250009"]}],"id":[{"id":"10.13039\/501100020884","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100020884","name":"Agencia Nacional de Investigaci\u00f3n y Desarrollo","doi-asserted-by":"publisher","award":["ANID BECAS DOCTORADO NACIONAL 21232432"],"award-info":[{"award-number":["ANID BECAS DOCTORADO NACIONAL 21232432"]}],"id":[{"id":"10.13039\/501100020884","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Edwardsiella anguillarum<\/jats:italic>\n                    is an emerging aquaculture pathogen responsible for economic losses across several cultured fish species. This study reports the isolation, identification, and characterisation of four isolates recovered from two infectious outbreaks in farmed Nile tilapia (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Oreochromis niloticus<\/jats:italic>\n                    <\/jats:styled-content>\n                    ) in Mexico. In 2021 and 2022, mortalities events occurred at two aquaculture facilities located in Quer\u00e9taro and Estado de M\u00e9xico, where affected fish exhibited clinical signs including skin darkening, ataxia, erratic swimming, haemorrhages at the operculum base, and scale loss. Internally, ascites and small nodules in the spleen and liver were observed. Bacteriological cultures from infected spleen tissue yielded four isolates, all conclusively identified as\n                    <jats:italic>E. anguillarum<\/jats:italic>\n                    through phenotypic, genetic, and phylogenomic analyses, clustering with the type strain\n                    <jats:italic>E. anguillarum<\/jats:italic>\n                    ET080813\n                    <jats:sup>T<\/jats:sup>\n                    , differentiating them from other\n                    <jats:italic>Edwardsiella<\/jats:italic>\n                    species. Draft genome sizes are approximately 4.12 Mbp, with GC contents of about 58.8%. Comparative genomics revealed near\u2010clonal relatedness among isolates, with Average Nucleotide Identity based on BLAST (ANIb) values &gt;\u200999.98% and identified 49 to 77 putative genomic islands enriched in mobility and virulence\u2010associated genes. Pathogenicity tests using isolate S\u2010002\u201023 resulted in mean cumulative mortality of 97% and 53% at 1.3\u2009\u00d7\u200910\n                    <jats:sup>6<\/jats:sup>\n                    and 3.7\u2009\u00d7\u200910\n                    <jats:sup>4<\/jats:sup>\n                    \u2009CFU\/fish, respectively. Infected tilapia displayed clinical signs and necrotising granulomas typical of edwardsiellosis. These findings expand the known geographic distribution of\n                    <jats:italic>E. anguillarum<\/jats:italic>\n                    , confirming its virulence and genomic plasticity, and suggesting a potential impact on tilapia aquaculture.\n                  <\/jats:p>","DOI":"10.1111\/jfd.70147","type":"journal-article","created":{"date-parts":[[2026,2,18]],"date-time":"2026-02-18T10:19:24Z","timestamp":1771409964000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Isolation and Comprehensive Characterisation of\n                    <i>Edwardsiella anguillarum<\/i>\n                    From Diseased Tilapia (\n                    <i>Oreochromis niloticus<\/i>\n                    ) Cultured in Mexico: Biochemical, Genetic and Genomic Insights and Dose\u2010Dependent Pathogenicity"],"prefix":"10.1111","volume":"49","author":[{"given":"Eduardo","family":"Mart\u00ednez\u2010Matus","sequence":"first","affiliation":[{"name":"Laboratorio de Patolog\u00eda de Organismos Acu\u00e1ticos y Biotecnolog\u00eda Acu\u00edcola, Facultad de Ciencias de la Vida Universidad Andr\u00e9s Bello  Vi\u00f1a del Mar Chile"},{"name":"Interdisciplinary Center for Aquaculture Research \u2013INCAR<sup>2<\/sup> Universidad Andr\u00e9s Bello  Vi\u00f1a del Mar Chile"},{"name":"Actino Lab USM, Centro de Biotecnolog\u00eda \u2018Daniel Alkalay Lowitt\u2019 y Departamento de Qu\u00edmica Universidad T\u00e9cnica Federico Santa Mar\u00eda  Valpara\u00edso Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4995-4676","authenticated-orcid":false,"given":"Cesar","family":"Ortega\u2010Santana","sequence":"additional","affiliation":[{"name":"Centro de Investigaci\u00f3n y Estudios Avanzados en Salud Animal, Facultad de Medicina Veterinaria y Zootecnia Universidad Aut\u00f3noma del Estado de M\u00e9xico  Toluca M\u00e9xico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00f3nica","family":"Saldarriaga\u2010C\u00f3rdoba","sequence":"additional","affiliation":[{"name":"Interdisciplinary Center for Aquaculture Research \u2013INCAR<sup>2<\/sup> Universidad Andr\u00e9s Bello  Vi\u00f1a del Mar Chile"},{"name":"Escuela de Medicina Veterinaria, Centro de Investigaci\u00f3n en Recursos Naturales y Sustentabilidad Universidad Bernardo O'Higgins  Santiago Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatriz","family":"C\u00e1mara","sequence":"additional","affiliation":[{"name":"Actino Lab USM, Centro de Biotecnolog\u00eda \u2018Daniel Alkalay Lowitt\u2019 y Departamento de Qu\u00edmica Universidad T\u00e9cnica Federico Santa Mar\u00eda  Valpara\u00edso Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5368-4475","authenticated-orcid":false,"given":"Ruben","family":"Avenda\u00f1o\u2010Herrera","sequence":"additional","affiliation":[{"name":"Laboratorio de Patolog\u00eda de Organismos Acu\u00e1ticos y Biotecnolog\u00eda Acu\u00edcola, Facultad de Ciencias de la Vida Universidad Andr\u00e9s Bello  Vi\u00f1a del Mar Chile"},{"name":"Interdisciplinary Center for Aquaculture Research \u2013INCAR<sup>2<\/sup> Universidad Andr\u00e9s Bello  Vi\u00f1a del Mar Chile"},{"name":"Centro de Investigaci\u00f3n Marina Quintay (CIMARQ) Universidad Andr\u00e9s Bello  Quintay Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,18]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0110726"},{"key":"e_1_2_12_3_1","unstructured":"AGRICULTURA.2021.\u201cAcuerdo mediante el cual se aprueba la actualizaci\u00f3n de la Carta Nacional Acu\u00edcola.\u201dDiario Oficial de la Federaci\u00f3n.https:\/\/dof.gob.mx\/nota_detalle.php?codigo=5615929&fecha=15\/04\/2021#gsc.tab=0."},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.13058"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.13691"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkx343"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msu088"},{"key":"e_1_2_12_8_1","volume-title":"BBMap: A Fast, Accurate, Splice\u2010Aware Aligner","author":"Bushnell B.","year":"2014"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.13743"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao02363"},{"key":"e_1_2_12_11_1","volume-title":"Performance Standards for Antimicrobial Susceptibility Testing of Bacteria Isolated From Aquatic Animals","author":"CLSI","year":"2020"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/jam.15538"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/17.19.7843"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2022.105620"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/femspd\/ftx058"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.14105"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2013.03.027"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao02687"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btt086"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2109"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2016.190"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw1004"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.00141"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12917\u2010015\u20100482\u2010x"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/bbx108"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts199"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.4194\/1303\u20102712\u2010v14_2_29"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa1105"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/are.14932"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/mlf2.12104"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/jwas.12576"},{"key":"e_1_2_12_32_1","volume-title":"Tilapia Aquaculture in Mexico: Assessment With a Focus on Social and Economic Performance","author":"Mart\u00ednez\u2010Cordero F. J.","year":"2021"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2025.107934"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens10081014"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.13135"},{"key":"e_1_2_12_36_1","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2020.00014"},{"key":"e_1_2_12_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.12775"},{"key":"e_1_2_12_38_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao02999"},{"key":"e_1_2_12_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-031-08491-1_3"},{"key":"e_1_2_12_40_1","doi-asserted-by":"publisher","DOI":"10.1101\/gr.186072.114"},{"key":"e_1_2_12_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/cpbi.102"},{"key":"e_1_2_12_42_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.13688"},{"key":"e_1_2_12_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.13982"},{"key":"e_1_2_12_44_1","doi-asserted-by":"publisher","DOI":"10.1128\/genomea.01004\u201015"},{"key":"e_1_2_12_45_1","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.00970\u201017"},{"key":"e_1_2_12_46_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btv681"},{"key":"e_1_2_12_47_1","unstructured":"SAGARPA.2018.\u201cAcuerdo mediante el cual se dan a conocer en los Estados Unidos Mexicanos las enfermedades y plagas ex\u00f3ticas y end\u00e9micas de notificaci\u00f3n obligatoria de los animales terrestres y acu\u00e1ticos.\u201dDiario Oficial de la Federaci\u00f3n.https:\/\/dof.gob.mx\/nota_detalle.php?codigo=5545304&fecha=29\/11\/2018#gsc.tab=0."},{"key":"e_1_2_12_48_1","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.molbev.a040454"},{"key":"e_1_2_12_49_1","doi-asserted-by":"publisher","DOI":"10.1577\/H08\u2010061.1"},{"key":"e_1_2_12_50_1","doi-asserted-by":"publisher","DOI":"10.1577\/H09\u2010004.1"},{"key":"e_1_2_12_51_1","unstructured":"SENASICA.2023.\u201cRequisitos para la Expedici\u00f3n de Certificado de Sanidad Acu\u00edcola para Instalaciones Acu\u00edcolas 2023 dem\u00e1s especies.\u201dhttps:\/\/www.gob.mx\/cms\/uploads\/attachment\/file\/826164\/Hoja_de_requisitos_para_Instalaciones_Acuicolas_2023_dem_s_especies.pdf."},{"key":"e_1_2_12_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.syapm.2014.10.008"},{"key":"e_1_2_12_53_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao067259"},{"issue":"4","key":"e_1_2_12_54_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.15381\/rivep.v31i4.17097","article-title":"Caracterizaci\u00f3n histol\u00f3gica y molecular de infecci\u00f3n por Edwardsiella anguillarum en tilapia (\n                     Oreochromis niloticus\n                  ) cultivada en sistema biofloc en Lima, Per\u00fa","volume":"31","author":"Sierralta V. A.","year":"2020","journal-title":"Revista de Investigaciones Veterinarias del Per\u00fa"},{"key":"e_1_2_12_55_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.13921"},{"key":"e_1_2_12_56_1","doi-asserted-by":"publisher","DOI":"10.1002\/aah.10169"},{"key":"e_1_2_12_57_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao03051"},{"key":"e_1_2_12_58_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/19.24.6823"},{"key":"e_1_2_12_59_1","doi-asserted-by":"publisher","DOI":"10.3147\/jsfp.34.145"},{"key":"e_1_2_12_60_1","doi-asserted-by":"publisher","DOI":"10.1111\/1755\u20100998.13096"}],"container-title":["Journal of Fish Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.70147","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jfd.70147","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.70147","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T14:28:24Z","timestamp":1780583304000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jfd.70147"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,18]]},"references-count":59,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2026,7]]}},"alternative-id":["10.1111\/jfd.70147"],"URL":"https:\/\/doi.org\/10.1111\/jfd.70147","archive":["Portico"],"relation":{},"ISSN":["0140-7775","1365-2761"],"issn-type":[{"value":"0140-7775","type":"print"},{"value":"1365-2761","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,18]]},"assertion":[{"value":"2025-10-23","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-31","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-18","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70147"}}