{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T20:27:36Z","timestamp":1783110456601,"version":"3.54.6"},"reference-count":32,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T00:00:00Z","timestamp":1771545600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T00:00:00Z","timestamp":1771545600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100005621","name":"Kasetsart University Research and Development Institute","doi-asserted-by":"publisher","award":["FF(KU) 56.69"],"award-info":[{"award-number":["FF(KU) 56.69"]}],"id":[{"id":"10.13039\/501100005621","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Infectious diseases have become a major challenge in global tilapia aquaculture. This study investigated disease outbreaks affecting red hybrid tilapia (\n                    <jats:italic>Oreochromis<\/jats:italic>\n                    spp.) by collecting 80 pooled samples from 36 farms between 2022 and 2024. Although the majority of samples tested positive for tilapia lake virus (TiLV), infectious spleen and kidney necrosis virus (ISKNV) was detected in 10% of the cases, specifically in fry and fingerlings originating from river\u2010based cage systems. All the ISKNV\u2010positive cases involved coinfections with TiLV, bacteria, and ectoparasites. Megalocytes with basophilic intracytoplasmic inclusion bodies were detected along with hemosiderin deposits in the anterior kidneys and spleens of the ISKNV\u2010positive fish. ISKNV was confirmed by PCR and in\u00a0situ hybridization. The virus was successfully isolated in red hybrid tilapia brain (RHTiB) cells, showing cytopathic effects within 2\u20135\u2009days postinfection. Phylogenetic analysis of the major capsid protein gene clustered the Thai isolate within ISKNV Clade 1, related to other Asian strains. Our findings highlight the need to include ISKNV in routine diagnostic surveillance and the importance of implementing integrated health management strategies focused on the early life stages of fish in intensive tilapia production systems.\n                  <\/jats:p>","DOI":"10.1111\/jfd.70150","type":"journal-article","created":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T05:36:11Z","timestamp":1771652171000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Investigation of Mortality Events Reveals Coinfections and Identification of Infectious Spleen and Kidney Necrosis Virus in Red Hybrid Tilapia in Thailand"],"prefix":"10.1111","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-9366-3709","authenticated-orcid":false,"given":"Benya","family":"Chatkaewchai","sequence":"first","affiliation":[{"name":"Department of Farm Resources and Production Medicine, Faculty of Veterinary Medicine Kasetsart University  Nakhon Pathom Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5735-9482","authenticated-orcid":false,"given":"Jidapa","family":"Yamkasem","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Veterinary Medicine Kasetsart University  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Piyaporn","family":"Eiamcharoen","sequence":"additional","affiliation":[{"name":"Department of Pathology, Faculty of Veterinary Medicine Kasetsart University  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Methanan","family":"Kamklang","sequence":"additional","affiliation":[{"name":"Kamphaeng Saen Veterinary Diagnostic Center, Faculty of Veterinary Medicine Kasetsart University  Nakhon Pathom 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Natural Sciences University of Stirling  Stirling UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-0154-6624","authenticated-orcid":false,"given":"Theeraporn","family":"Pulpipat","sequence":"additional","affiliation":[{"name":"Department of Farm Resources and Production Medicine, Faculty of Veterinary Medicine Kasetsart University  Nakhon Pathom Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,2,20]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.17605"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2017.11.019"},{"issue":"1","key":"e_1_2_12_4_1","first-page":"11","article-title":"Distribution and Detection of Megalocytivirus in Ornamental Fishes in Thailand","volume":"43","author":"Baoprasertkul P.","year":"2019","journal-title":"Journal of Fisheries and 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