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Therefore, developing effective antiviral agents against this viral infection is crucial for the largemouth bass aquaculture industry. Quercetin, a widely utilised flavonoid known for its therapeutic and prophylactic properties, was investigated for its antiviral efficacy against LMBV and underlying mechanisms in this study. The results demonstrated that quercetin significantly inhibited LMBV propagation in\u00a0vitro and in\u00a0vivo, and was effective in both prevention and treatment stages. Specifically, quercetin at 6.25\u2009\u03bcg\/mL suppressed LMBV (major capsid protein)\n                    <jats:italic>MCP<\/jats:italic>\n                    gene transcription by up to 92.09%, with the post\u2010infection treatment achieving 99.33% inhibition in\u00a0vitro. Mechanistic studies revealed that quercetin reduces LMBV infectivity by disrupting viral internalisation and replication, while also potentiating cellular immune responses mediated by the IFN system. In\u00a0vivo experiments further confirmed that quercetin treatment reduced viral transcription in the liver, spleen and kidney by 97.49%, 47.45% and 98.42%, respectively, at 48\u2009h post\u2010infection and significantly enhanced the survival rate of infected juveniles. In conclusion, quercetin demonstrated potent antiviral activity against LMBV and might serve as a promising, eco\u2010friendly antiviral agent.\n                  <\/jats:p>","DOI":"10.1111\/jfd.70152","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T08:56:13Z","timestamp":1772960173000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["The Antiviral Activity and Underlying Mechanisms of Quercetin in Combating Largemouth Bass Virus Infection"],"prefix":"10.1111","volume":"49","author":[{"given":"Lin","family":"Huang","sequence":"first","affiliation":[{"name":"Guangxi Academy of Marine Sciences, Guangxi Academy of Sciences, Guangxi Key Laboratory of Aquatic Biotechnology and Modern Ecological Aquaculture, Guangxi Engineering Research Center for Fishery Major Diseases Control and Efficient Healthy Breeding Industrial Technology  Nanning People's Republic of 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