{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T20:27:26Z","timestamp":1783110446507,"version":"3.54.6"},"reference-count":40,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T00:00:00Z","timestamp":1772841600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T00:00:00Z","timestamp":1772841600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32473146"],"award-info":[{"award-number":["32473146"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFD2401603"],"award-info":[{"award-number":["2023YFD2401603"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Cell line misidentification or contamination undermines the reliability of research outcomes. In this study, we verified the species origin of commonly used fish cell lines, with a particular focus on the grass carp ovarian cell line (GCO). RNA\u2010seq data from 146 samples of 11 widely used fish cell lines were collected from public databases (NCBI\u2010SRA) and were mapped to the reference genomes of eight species. GCO RNA\u2010seq samples from three Chinese laboratories showed a mapping rate of 21.64%\u2009\u00b1\u20093.61% to the grass carp genome, but 89.81%\u2009\u00b1\u20094.83% to the fathead minnow genome. Further transcriptomic similarity analysis revealed that these GCO RNA\u2010seq samples clustered more closely with cell lines originating from fathead minnow. Targeted analysis of the COI gene amplification region confirmed that nearly all reads from GCO RNA\u2010seq samples mapped exclusively to the fathead minnow COI amplicon. Collectively, these results indicate that GCO may have undergone cross\u2010contamination or species misidentification. This study raises doubts about the true species origin of the GCO cell line and proposes that COI DNA barcoding can be used to discriminate the species origin of fish cell lines.<\/jats:p>","DOI":"10.1111\/jfd.70153","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T15:06:00Z","timestamp":1772982360000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Survey of the Species Origins of Common Fish Cell Lines\u2014With a Particular Focus on the Grass Carp Ovarian Cell Line (\n                    <scp>GCO<\/scp>\n                    )"],"prefix":"10.1111","volume":"49","author":[{"given":"Hong","family":"Yang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture (CAS), Hubei Hongshan Laboratory, Key Laboratory of Aquaculture Disease Control, Ministry of Agriculture and Rural Affairs, the Innovation Academy of Seed 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