{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T15:53:42Z","timestamp":1785772422095,"version":"3.56.0"},"reference-count":40,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T00:00:00Z","timestamp":1774396800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T00:00:00Z","timestamp":1774396800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Nocardiosis is a chronic bacterial disease causing significant losses in marine aquaculture worldwide. This study reports the first confirmed outbreak of nocardiosis caused by\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Nocardia seriolae<\/jats:italic>\n                    <\/jats:styled-content>\n                    in farmed spotted scat (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Scatophagus argus<\/jats:italic>\n                    <\/jats:styled-content>\n                    ) in Vietnam, with cumulative mortalities reaching up to 70%. Diseased fish exhibited skin ulceration and disseminated white nodules in internal organs and musculature. Histopathological examination revealed typical granulomatous lesions containing abundant Gram\u2010positive, branching filamentous bacteria. A bacterium with consistent morphological characteristics was isolated from affected organs, and molecular identification based on 16S rRNA gene sequencing and species\u2010specific PCR confirmed the pathogen as\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>N. seriolae<\/jats:italic>\n                    <\/jats:styled-content>\n                    . The experimental challenge reproduced the clinical signs and pathological features of natural infection, with an estimated LD50 of 5.3\u2009\u00d7\u200910\n                    <jats:sup>3<\/jats:sup>\n                    \u2009CFU\/fish, confirming high pathogenicity in this host. Antimicrobial susceptibility testing showed reduced susceptibility to amoxicillin and universal reduced susceptibility to trimethoprim\u2013sulfamethoxazole, while all isolates remained wild type or susceptible to florfenicol, oxytetracycline, erythromycin, and doxycycline based on previously reported cut\u2010off values. This study extends the known host range of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>N. seriolae<\/jats:italic>\n                    <\/jats:styled-content>\n                    and highlights nocardiosis as an emerging disease threat to spotted scat aquaculture, emphasising the need for early diagnosis and targeted health management strategies.\n                  <\/jats:p>","DOI":"10.1111\/jfd.70171","type":"journal-article","created":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T03:55:47Z","timestamp":1774497347000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["First Report of Nocardiosis Caused by\n                    <i>Nocardia seriolae<\/i>\n                    in Farmed Spotted Scat (\n                    <i>Scatophagus argus<\/i>\n                    )"],"prefix":"10.1111","volume":"49","author":[{"given":"Anh Tuan","family":"Nguyen","sequence":"first","affiliation":[{"name":"Faculty of Fisheries University of Agriculture and Forestry, Hue University  Hu\u1ebf Th\u1eeba Thi\u00ean Hu\u1ebf Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dang Thi","family":"Hoa","sequence":"additional","affiliation":[{"name":"Faculty of Fisheries Vietnam National University of Agriculture  Hanoi Hanoi Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nguyen Vu","family":"Son","sequence":"additional","affiliation":[{"name":"Faculty of Veterinary Medicine Vietnam National University of Agriculture  Hanoi Hanoi Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doan Thi","family":"Nhinh","sequence":"additional","affiliation":[{"name":"Faculty of Fisheries Vietnam National University of Agriculture  Hanoi Hanoi Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Le Viet","family":"Dung","sequence":"additional","affiliation":[{"name":"Faculty of Fisheries Vietnam National University of Agriculture  Hanoi Hanoi Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2271-849X","authenticated-orcid":false,"given":"Truong Dinh","family":"Hoai","sequence":"additional","affiliation":[{"name":"Faculty of Fisheries Vietnam National University of Agriculture  Hanoi Hanoi Vietnam"},{"name":"Center of Excellence and Innovation Vietnam National University of Agriculture  Hanoi Hanoi Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,25]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2023.740458"},{"key":"e_1_2_11_3_1","first-page":"4","volume-title":"Spawning Induction and Pond Culture of the Spotted Scat (Scatophagus Argus Linnaeus) in The Philippines","author":"Barry T.","year":"1988"},{"issue":"1992","key":"e_1_2_11_4_1","first-page":"163","article-title":"Biology of the Spotted Scat (Scatophagus Argus) in The Philippines","volume":"5","author":"Barry T. 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