{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T01:06:54Z","timestamp":1788052014762,"version":"build-2784847793"},"reference-count":52,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T00:00:00Z","timestamp":1774310400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T00:00:00Z","timestamp":1774310400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Cyprinid herpesvirus 2 (CyHV\u20102) is a major pathogen causing high mortality in farmed goldfish and crucian carp, for which effective prophylactic or therapeutic treatments are currently limited. Lauric acid (LA) and glycerol monolaurate (GML), representative of a medium\u2010chain fatty acid (MCFA) and its corresponding monoglyceride, respectively, have been reported to possess antiviral and immunomodulatory properties. This study systematically evaluated the anti\u2010CyHV\u20102 effects of LA and GML through in\u00a0vitro assays, transcriptomic profiling, and in\u00a0vivo experiments. In\u00a0vitro, both LA and GML significantly reduced viral copy numbers in gibel carp caudal fin (GiCF) cells, attenuated virus\u2010induced cytopathic effects (CPE), and suppressed intracellular viral replication. Transcriptomic analysis revealed that LA and GML induced widespread alterations in host signalling pathways, with significant enrichment of pathways related to steroid biosynthesis, ECM\u2013receptor interaction, protein digestion and absorption, and cell survival\u2013associated signalling. Quantitative PCR analysis further demonstrated that the expression levels of\n                    <jats:italic>insr<\/jats:italic>\n                    ,\n                    <jats:italic>akt2<\/jats:italic>\n                    ,\n                    <jats:italic>pdk1<\/jats:italic>\n                    ,\n                    <jats:italic>lamtor3<\/jats:italic>\n                    , and\n                    <jats:italic>hsp90b<\/jats:italic>\n                    were significantly upregulated, whereas\n                    <jats:italic>inpp4b<\/jats:italic>\n                    expression was downregulated. These results further validate that host cell metabolic processes, stress responses, and signal transduction pathways are substantially modulated following LA and GML treatment. The in\u00a0vivo protective efficacy of GML was further assessed. In goldfish infected with CyHV\u20102, GML administration significantly increased survival rate and mitigated histopathological damage in the gills, liver, spleen, and kidney. At 3\u2009days post\u2010infection (dpi), expression levels of pro\u2010inflammatory cytokines\n                    <jats:italic>il\u20101\u03b2<\/jats:italic>\n                    ,\n                    <jats:italic>il\u20106<\/jats:italic>\n                    , and\n                    <jats:italic>tnf\u2010\u03b1<\/jats:italic>\n                    were significantly lower in the GML\u2010treated group compared to the virus\u2010infected control group, whereas the anti\u2010inflammatory cytokine\n                    <jats:italic>il\u201010<\/jats:italic>\n                    was significantly upregulated. By 7 dpi, differences in inflammatory cytokine expression between groups had diminished, suggesting that GML not only exerts direct antiviral activity but may also modulate host immune responses during the early stage of infection. Collectively, these findings provide a theoretical basis for the practical application of LA and GML and propose a novel strategy for developing safe, effective, and environmentally friendly anti\u2010CyHV\u20102 agents.\n                  <\/jats:p>","DOI":"10.1111\/jfd.70174","type":"journal-article","created":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T04:20:28Z","timestamp":1774412428000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Lauric Acid and Glycerol Monolaurate Inhibit the Activity of Cyprinid Herpesvirus 2 and Enhance Host Immune Reaction"],"prefix":"10.1111","volume":"49","author":[{"given":"Jiahua","family":"Zhang","sequence":"first","affiliation":[{"name":"National Pathogen Collection Center for Aquatic Animals Shanghai Ocean University  Shanghai China"},{"name":"Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education Shanghai Ocean University  Shanghai China"},{"name":"Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs Shanghai Ocean University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Qin","sequence":"additional","affiliation":[{"name":"National Pathogen Collection Center for Aquatic Animals Shanghai Ocean University  Shanghai China"},{"name":"Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education Shanghai Ocean University  Shanghai China"},{"name":"Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs Shanghai Ocean University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mingyang","family":"Ma","sequence":"additional","affiliation":[{"name":"National Pathogen Collection Center for Aquatic Animals Shanghai Ocean University  Shanghai China"},{"name":"Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education Shanghai Ocean University  Shanghai China"},{"name":"Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs Shanghai Ocean University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tonghui","family":"Xu","sequence":"additional","affiliation":[{"name":"National Pathogen Collection Center for Aquatic Animals Shanghai Ocean University  Shanghai China"},{"name":"Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education Shanghai Ocean University  Shanghai China"},{"name":"Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs Shanghai Ocean University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"Zhang","sequence":"additional","affiliation":[{"name":"National Pathogen Collection Center for Aquatic Animals Shanghai Ocean University  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