{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T15:23:37Z","timestamp":1788276217812,"version":"build-2803163510"},"reference-count":37,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T00:00:00Z","timestamp":1777420800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T00:00:00Z","timestamp":1777420800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100012547","name":"Natural Science Foundation of Guangxi Zhuang Autonomous Region","doi-asserted-by":"publisher","award":["AD23026331"],"award-info":[{"award-number":["AD23026331"]}],"id":[{"id":"10.13039\/100012547","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100012547","name":"Natural Science Foundation of Guangxi Zhuang Autonomous Region","doi-asserted-by":"publisher","award":["2022JJA130281"],"award-info":[{"award-number":["2022JJA130281"]}],"id":[{"id":"10.13039\/100012547","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32160873"],"award-info":[{"award-number":["32160873"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Tilapia Lake Virus (TiLV) is an emerging pathogen that poses a significant threat to global tilapia aquaculture, leading to substantial economic losses. The development of rapid, sensitive, and specific diagnostic methods is crucial for disease control and prevention. In this study, TiLV\u2010infected tilapia brain cell line (TiB cells) was used as the target for screening specific aptamers via the Systematic Evolution of Ligands by Exponential Enrichment (SELEX) technique. The affinity, specificity, stability, cytotoxicity, and antiviral activity of the obtained aptamers were systematically analysed through flow cytometry, confocal laser scanning microscopy, real\u2010time quantitative PCR, and cell viability assays. This study successfully screened and obtained two aptamers, LH\u20101 and LH\u20102. Both aptamers specifically recognize and bind to TiLV\u2010infected TiB cells while showing no cross\u2010reactivity with normal cells or cells infected with other viruses. Their secondary structures exhibit typical stem\u2010loop configurations, with dissociation constants (K\n                    <jats:italic>d<\/jats:italic>\n                    ) reaching nanomolar levels at 506.9\u2009nM and 326.4\u2009nM, respectively, and they demonstrate no cytotoxicity toward host cells. Aptamer LH\u20102 is capable of detecting as few as 1\u2009\u00d7\u200910\n                    <jats:sup>3<\/jats:sup>\n                    cells\/mL within 5\u2009min at temperatures ranging from 4\u00b0C to 28\u00b0C. The detection capability of LH\u20102 for TiLV infection was consistent with that of RT\u2010qPCR, as validated in\u00a0vivo. These results demonstrate that LH\u20102 possesses significant potential to be developed as a core recognition component for on\u2010site rapid detection kits. This study provides critical molecular tools and a theoretical foundation for the rapid diagnosis of TiLV and the development of novel targeted prevention and control strategies. It strongly advances the practical application of aptamer technology in the precise prevention and control of aquatic diseases.\n                  <\/jats:p>","DOI":"10.1111\/jfd.70190","type":"journal-article","created":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T05:36:42Z","timestamp":1777527402000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Screening and Application of Novel\n                    <scp>DNA<\/scp>\n                    Aptamers for Targeting Tilapia Lake Virus\u2010Infected Cells"],"prefix":"10.1111","volume":"49","author":[{"given":"Mingzhu","family":"Liu","sequence":"first","affiliation":[{"name":"Guangxi Academy of Marine Sciences, Guangxi Key Laboratory of Aquatic Biotechnology and Modern Ecological Aquaculture, Guangxi Engineering Research Center for Fishery Major Diseases Control and Efffcient Healthy Breeding Industrial Technology (GERCFT), Guangxi Academy of Sciences  Nanning P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peiyu","family":"Li","sequence":"additional","affiliation":[{"name":"Ningbo Institute of Technology, Zhejiang University  Ningbo P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guixiang","family":"Tong","sequence":"additional","affiliation":[{"name":"Guangxi Academy of Fishery Science  Nanning Guangxi P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinxian","family":"Wei","sequence":"additional","affiliation":[{"name":"Guangxi Academy of Fishery Science  Nanning Guangxi P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7139-1454","authenticated-orcid":false,"given":"Junxiang","family":"Lai","sequence":"additional","affiliation":[{"name":"Guangxi Academy of Marine Sciences, Guangxi Key Laboratory of Aquatic Biotechnology and Modern Ecological Aquaculture, Guangxi Engineering Research Center for Fishery Major Diseases Control and Efffcient Healthy Breeding Industrial Technology (GERCFT), Guangxi Academy of Sciences  Nanning P.R. China"},{"name":"Chinese Ministry of Agriculture and Rural Affairs Aquatic and Livestock Farming Field Scientific Observation and Research Station (Nanning), China\u2010ASEAN Modern Fishery Industry Technology Transfer Demonstration Center Beibu Gulf Marine Industrial Research Institute  Nanning P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ermeng","family":"Yu","sequence":"additional","affiliation":[{"name":"Guangxi Academy of Marine Sciences, Guangxi Key Laboratory of Aquatic Biotechnology and Modern Ecological Aquaculture, Guangxi Engineering Research Center for Fishery Major Diseases Control and Efffcient Healthy Breeding Industrial Technology (GERCFT), Guangxi Academy of Sciences  Nanning P.R. China"},{"name":"Chinese Ministry of Agriculture and Rural Affairs Aquatic and Livestock Farming Field Scientific Observation and Research Station (Nanning), China\u2010ASEAN Modern Fishery Industry Technology Transfer Demonstration Center Beibu Gulf Marine Industrial Research Institute  Nanning P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pengfei","family":"Li","sequence":"additional","affiliation":[{"name":"Guangxi Academy of Marine Sciences, Guangxi Key Laboratory of Aquatic Biotechnology and Modern Ecological Aquaculture, Guangxi Engineering Research Center for Fishery Major Diseases Control and Efffcient Healthy Breeding Industrial Technology (GERCFT), Guangxi Academy of Sciences  Nanning P.R. China"},{"name":"Chinese Ministry of Agriculture and Rural Affairs Aquatic and Livestock Farming Field Scientific Observation and Research Station (Nanning), China\u2010ASEAN Modern Fishery Industry Technology Transfer Demonstration Center Beibu Gulf Marine Industrial Research Institute  Nanning P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lichun","family":"Huang","sequence":"additional","affiliation":[{"name":"Guangxi Academy of Marine Sciences, Guangxi Key Laboratory of Aquatic Biotechnology and Modern Ecological Aquaculture, Guangxi Engineering Research Center for Fishery Major Diseases Control and Efffcient Healthy Breeding Industrial Technology (GERCFT), Guangxi Academy of Sciences  Nanning P.R. China"},{"name":"Nanning Aquatic Animal Husbandry and Veterinary Technology Station  Nanning P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Yu","sequence":"additional","affiliation":[{"name":"Guangxi Academy of Marine Sciences, Guangxi Key Laboratory of Aquatic Biotechnology and Modern Ecological Aquaculture, Guangxi Engineering Research Center for Fishery Major Diseases Control and Efffcient Healthy Breeding Industrial Technology (GERCFT), Guangxi Academy of Sciences  Nanning P.R. China"},{"name":"Chinese Ministry of Agriculture and Rural Affairs Aquatic and Livestock Farming Field Scientific Observation and Research Station (Nanning), China\u2010ASEAN Modern Fishery Industry Technology Transfer Demonstration Center Beibu Gulf Marine Industrial Research Institute  Nanning P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,29]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1128\/mra.01368\u201019"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aaf.2021.04.007"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41437\u2010021\u201000447\u20104"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.3969\/j.issn.1005\u2010944X.2017.10.017"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2022.114567"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010023\u201047425\u2010w"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2017.06.035"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2017.04.019"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2022.739166"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010024\u201053619\u20107"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2024.133935"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.13657\/j.cnki.gxkxyxb.20241226.003"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.3c00377"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12917\u2010015\u20100559\u20106"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.13674"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.aquaculture.2025.742768"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12985\u2010025\u201002657\u20102"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.adv6127"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2020.10.019"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2021.06.017"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.2174\/1568009622666220214101059"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/v14061242"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.13693"},{"key":"e_1_2_11_25_1","first-page":"1787","article-title":"Action Mechanism of Semen Ziziphi spinosae Alcohol Extracts and Betulinic Acid Against Nervous Necrosis Virus","volume":"56","author":"Xu W. 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