{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T15:23:27Z","timestamp":1788276207727,"version":"build-2803163510"},"reference-count":33,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:00:00Z","timestamp":1777507200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:00:00Z","timestamp":1777507200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>The infectious hypodermal and haematopoietic necrosis virus (IHHNV) represents a significant viral threat to global shrimp aquaculture, leading to considerable economic losses. In this study, we have developed a one\u2010step, one\u2010pot isothermal assay for the detection of IHHNV, employing recombinase\u2010aided amplification in conjunction with clustered regularly interspaced short palindromic repeats\u2010Cas12a (RAA\u2010CRISPR\/Cas12a). The assay is performed at a constant temperature of 37\u00b0C, achieving a detection limit of 10 copies per reaction for the fluorescence assay and 1 copy per reaction for the lateral flow dipstick (LFD) assay within a 60\u2010min timeframe. Additionally, we evaluated the assay against four other prevalent shrimp pathogens (WSSV, DIV1, EHP, VpAHPND) and observed no cross\u2010reactivity. This straightforward detection method exhibits high sensitivity and specificity for IHHNV, offering a promising approach for early and rapid field diagnosis.<\/jats:p>","DOI":"10.1111\/jfd.70196","type":"journal-article","created":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T05:01:53Z","timestamp":1777611713000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["One\u2010Pot\n                    <scp>RAA<\/scp>\n                    \u2010\n                    <scp>CRISPR<\/scp>\n                    \/Cas12a Assay for Rapid Detection of Infectious Hypodermal and Haematopoietic Necrosis Virus (\n                    <scp>IHHNV<\/scp>\n                    ) in Shrimp Aquaculture"],"prefix":"10.1111","volume":"49","author":[{"given":"Zewei","family":"Hou","sequence":"first","affiliation":[{"name":"National Pathogen Collection Center for Aquatic Animals Shanghai Ocean University  Shanghai People's Republic of China"},{"name":"Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education Shanghai Ocean University  Shanghai People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yichen","family":"Zhao","sequence":"additional","affiliation":[{"name":"National Pathogen Collection Center for Aquatic Animals Shanghai Ocean University  Shanghai People's Republic of China"},{"name":"Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education Shanghai Ocean University  Shanghai People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Sun","sequence":"additional","affiliation":[{"name":"National Pathogen Collection Center for Aquatic Animals Shanghai Ocean University  Shanghai People's Republic of China"},{"name":"Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education Shanghai Ocean University  Shanghai People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Zhan","sequence":"additional","affiliation":[{"name":"National Pathogen Collection Center for Aquatic Animals Shanghai Ocean University  Shanghai People's Republic of China"},{"name":"Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education Shanghai Ocean University  Shanghai People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0595-9715","authenticated-orcid":false,"given":"Xilin","family":"Dai","sequence":"additional","affiliation":[{"name":"National Pathogen Collection Center for Aquatic Animals Shanghai Ocean University  Shanghai People's Republic of China"},{"name":"Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education Shanghai Ocean University  Shanghai People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8893-1568","authenticated-orcid":false,"given":"Hao","family":"Wang","sequence":"additional","affiliation":[{"name":"National Pathogen Collection Center for Aquatic Animals Shanghai Ocean University  Shanghai People's Republic of China"},{"name":"Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education Shanghai Ocean University  Shanghai People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,30]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/aah.10218"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1128\/mra.01143\u201021"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao060089"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jip.2018.08.009"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aar6245"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid2802.211874"},{"key":"e_1_2_10_8_1","first-page":"85","volume-title":"Advances in Virus Research","author":"Dhar A. 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