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The Senegalese sole (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Solea senegalensis<\/jats:italic>\n                    <\/jats:styled-content>\n                    ), a relevant species for Spanish aquaculture diversification, lacks established in\u00a0vitro models. We developed SsB\u20101, the first brain\u2010derived cell line from this species, providing a valuable platform for studying virus\u2013host interactions. SsB\u20101 exhibits glial features, stable morphology, and high susceptibility to several betanodavirus (NNV) genotypes, enabling detailed analysis of neurotropism and virulence mechanisms. Its selective permissiveness to NNV and limited replication of infectious pancreatic necrosis virus (IPNV), spring viremia of carp (SVCV), and rhabdovirus eel virus European X (EVEX) highlight its versatility. The ability of some viruses to enter cells without triggering cytopathic or antiviral responses also points to new research avenues for potential reservoir roles in flatfish. Overall, SsB\u20101 offers a robust, ethical, and versatile model to advance virological and immunological research in support of sustainable marine aquaculture.\n                  <\/jats:p>","DOI":"10.1111\/jfd.70197","type":"journal-article","created":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T08:30:57Z","timestamp":1777365057000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Expanding the Fish\u2010Brain Invitrome With the Senegalese Sole\n                    <scp>SsB<\/scp>\n                    \u20101 Cell Line\u2014A Versatile Model for Neurotropic Virus Research"],"prefix":"10.1111","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5432-4903","authenticated-orcid":false,"given":"Yulema","family":"Valero","sequence":"first","affiliation":[{"name":"Immunobiology for Aquaculture Group, Department of Cell Biology and Histology, Faculty of Biology University of Murcia  Murcia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0906-7995","authenticated-orcid":false,"given":"Alberto","family":"Cuesta","sequence":"additional","affiliation":[{"name":"Immunobiology for Aquaculture Group, Department of Cell Biology and Histology, Faculty of Biology University of Murcia  Murcia Spain"},{"name":"Marine Science Institute (UMUMAR)  Murcia Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,28]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/pathogens9020106"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmars.2022.1038196"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11626\u2010017\u20100142\u20105"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005203"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014\u20104827(77)90089\u20101"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao065091"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10126\u2010025\u201010507\u2010z"},{"key":"e_1_2_11_9_1","first-page":"87","volume-title":"Fish Diseases","author":"Dixon P. 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