{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T15:23:45Z","timestamp":1788276225715,"version":"build-2803163510"},"reference-count":51,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T00:00:00Z","timestamp":1777939200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T00:00:00Z","timestamp":1777939200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003392","name":"Natural Science Foundation of Fujian Province","doi-asserted-by":"publisher","award":["2023J011070"],"award-info":[{"award-number":["2023J011070"]}],"id":[{"id":"10.13039\/501100003392","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The bacterial pathogen\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Pseudomonas plecoglossicida<\/jats:italic>\n                    <\/jats:styled-content>\n                    causes visceral white nodules disease (VWND) in the large yellow croaker (LYC;\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Larimichthys crocea<\/jats:italic>\n                    <\/jats:styled-content>\n                    ), a commonly farmed species in China, resulting in substantial economic losses in the aquaculture industry. To better understand host innate immune mechanisms during\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. plecoglossicida<\/jats:italic>\n                    <\/jats:styled-content>\n                    infection, LYCs were infected either by intraperitoneal (IP) injection or immersion, and their non\u2010specific immune responses were compared. Fish were subjected to serial concentrations of the pathogen in both challenged groups, and the median lethal dose (LD\n                    <jats:sub>50<\/jats:sub>\n                    ) was determined for subsequent infection trials. The results show that the respiratory burst activity, antioxidant activity (SOD, CAT and MDA), and non\u2010specific immune responses (ACP, ALP and LZM) of LYCs were stimulated, and the expression levels of inflammatory cytokines (\n                    <jats:italic>il\u20101\u03b2<\/jats:italic>\n                    ,\n                    <jats:italic>tnf\u03b11<\/jats:italic>\n                    ,\n                    <jats:italic>il\u20108<\/jats:italic>\n                    and\n                    <jats:italic>il\u201010<\/jats:italic>\n                    ) were considerably upregulated compared with mock\u2010infected fish during bacterial infection periods via both challenge routes. Furthermore, the non\u2010specific response in the immersion group started later and appeared to last longer, often exceeding the response in IP\u2010injected fish. These results will contribute to a further understanding of the pathogenesis and host defence system in fishes following infection with\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>P. plecoglossicida<\/jats:italic>\n                    <\/jats:styled-content>\n                    , and may inform the future development of preventive strategies for controlling VWND.\n                  <\/jats:p>","DOI":"10.1111\/jfd.70201","type":"journal-article","created":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T01:58:14Z","timestamp":1778032694000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Non\u2010Specific Immune Response in Large Yellow Croaker (\n                    <i>Larimichthys crocea<\/i>\n                    ) Experimentally Infected With\n                    <i>Pseudomonas plecoglossicida<\/i>\n                    by Intraperitoneal Injection or Immersion"],"prefix":"10.1111","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9886-7355","authenticated-orcid":false,"given":"Liangyin","family":"He","sequence":"first","affiliation":[{"name":"College of Marine Sciences, Ningde Normal University  Ningde China"},{"name":"Engineering Research Center of Mindong Aquatic Product Deep\u2010Processing, Fujian Province University  Ningde China"},{"name":"Fujian Province Key Laboratory of Special Aquatic Formula Feed, Fujian Tianma Science and Technology Group Co., Ltd.  Fuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rongguang","family":"Zhong","sequence":"additional","affiliation":[{"name":"College of Marine Sciences, Ningde Normal University  Ningde China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Doudou","family":"Wu","sequence":"additional","affiliation":[{"name":"College of Marine Sciences, Ningde Normal University  Ningde China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junkun","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Marine Sciences, Ningde Normal University  Ningde China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinling","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Marine Sciences, Ningde Normal University  Ningde China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8075-7665","authenticated-orcid":false,"given":"Weiqing","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Marine Sciences, Ningde Normal University  Ningde China"},{"name":"Engineering Research Center of Mindong Aquatic Product Deep\u2010Processing, Fujian Province University  Ningde China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,5]]},"reference":[{"key":"e_1_2_11_2_1","first-page":"107","article-title":"A New Pseudomonas Species Observed in Cultured Young Rainbow Trout (Oncorhynchus mykis Walbaum, 1792): \n                     Pseudomonas plecoglossicida","volume":"4","author":"Akayli T.","year":"2011","journal-title":"Biyoloji Bilimleri Ara\u015ft\u0131rma 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