{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T15:23:47Z","timestamp":1788276227282,"version":"build-2803163510"},"reference-count":37,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T00:00:00Z","timestamp":1778630400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T00:00:00Z","timestamp":1778630400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100014188","name":"Ministry of Science and ICT, South Korea","doi-asserted-by":"publisher","award":["RS\u20102024\u201000405986"],"award-info":[{"award-number":["RS\u20102024\u201000405986"]}],"id":[{"id":"10.13039\/501100014188","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Vibrio anguillarum<\/jats:italic>\n                    <\/jats:styled-content>\n                    infection poses a significant threat to freshwater and marine aquaculture. Although whole\u2010cell killed and live attenuated vaccines are used to control vibriosis, their comparative efficacy against homologous and heterologous\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>V. anguillarum<\/jats:italic>\n                    <\/jats:styled-content>\n                    strains remains limited. Here, we compared the protective efficacy and antibody responses of a formalin\u2010killed\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>V. anguillarum<\/jats:italic>\n                    <\/jats:styled-content>\n                    (FKC) and an alanine racemase gene knockout auxotrophic\u2010live\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>V. anguillarum<\/jats:italic>\n                    <\/jats:styled-content>\n                    (\u0394\n                    <jats:italic>alr1<\/jats:italic>\n                    \u0394\n                    <jats:italic>\n                      alr2\n                      <jats:styled-content style=\"fixed-case\">V. anguillarum<\/jats:styled-content>\n                    <\/jats:italic>\n                    , LAV), both derived from the\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>V. anguillarum<\/jats:italic>\n                    <\/jats:styled-content>\n                    JJ strain, in rainbow trout against homologous (JJ strain) and heterologous (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>V. anguillarum<\/jats:italic>\n                    <\/jats:styled-content>\n                    90\u201011\u2010287) strains. The LAV conferred superior protection compared to the FKC against both strains under high\u2010dose challenge conditions. Despite this, ELISA antibody levels were comparable between vaccine groups and did not correlate with protective outcomes. No agglutination activity against the JJ strain was detected in vaccinated fish or rabbit antisera, suggesting limited assay sensitivity rather than absence of antibody binding. Analysis of the O\u2010antigen biosynthesis\u2010related genes revealed no genetic differences between strains; however, the JJ strain exhibited a shorter O\u2010antigen, which may contribute to the reduced agglutination of the JJ strain. In conclusion, our results underscore the need for a comprehensive evaluation of vaccine performance, extending beyond antibody quantification, when developing effective vaccination strategies for aquaculture.\n                  <\/jats:p>","DOI":"10.1111\/jfd.70210","type":"journal-article","created":{"date-parts":[[2026,5,14]],"date-time":"2026-05-14T05:19:46Z","timestamp":1778735986000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of Protective Efficacy of Formalin\u2010Killed and Auxotrophic\u2010Live\n                    <scp>\n                      <i>Vibrio anguillarum<\/i>\n                    <\/scp>\n                    Against Homologous and Heterologous Strains in Rainbow Trout (\n                    <scp>\n                      <i>Oncorhynchus mykiss<\/i>\n                    <\/scp>\n                    )"],"prefix":"10.1111","volume":"49","author":[{"given":"Kyung 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