{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T18:02:29Z","timestamp":1792605749591,"version":"4.5.0"},"reference-count":38,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T00:00:00Z","timestamp":1779753600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T00:00:00Z","timestamp":1779753600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100012547","name":"Natural Science Foundation of Guangxi Zhuang Autonomous Region","doi-asserted-by":"publisher","award":["2024GXNSFAA010004"],"award-info":[{"award-number":["2024GXNSFAA010004"]}],"id":[{"id":"10.13039\/100012547","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2023M732889"],"award-info":[{"award-number":["2023M732889"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002858","name":"China Postdoctoral Science Foundation","doi-asserted-by":"publisher","award":["2024T170733"],"award-info":[{"award-number":["2024T170733"]}],"id":[{"id":"10.13039\/501100002858","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2026,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Fas\u2010associated death domain protein (FADD) was initially identified for its involvement in apoptosis signal transduction, while subsequent research has revealed its additional functions in proliferation, cell cycle regulation, inflammation, and innate immunity. However, current research on\n                    <jats:italic>FADD<\/jats:italic>\n                    predominantly focuses on mammals, with only limited studies conducted on fish. In this study, we cloned\n                    <jats:italic>FADD<\/jats:italic>\n                    from Nile tilapia (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Oreochromis niloticus<\/jats:italic>\n                    <\/jats:styled-content>\n                    ) and analysed its sequence characteristics. Subsequently, qPCR was used to detect the gene expression of\n                    <jats:italic>FADD<\/jats:italic>\n                    in healthy tissues and its changes in\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Streptococcus agalactiae<\/jats:italic>\n                    <\/jats:styled-content>\n                    infected tissues. After obtaining FADD through prokaryotic expression, its ability to bind to\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>S. agalactiae<\/jats:italic>\n                    <\/jats:styled-content>\n                    was assessed using ELISA. Finally, the growth of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>S. agalactiae<\/jats:italic>\n                    <\/jats:styled-content>\n                    was assessed by adding FADD to the bacterial culture medium. The results indicate that the coding sequence of\n                    <jats:italic>FADD<\/jats:italic>\n                    spans 585\u2009bp, encoding 194 amino acids. Predicted to be an intracellular protein, FADD exhibits high hydrophilicity and lacks transmembrane regions, with a structure predominantly composed of \u03b1\u2010helices.\n                    <jats:italic>FADD<\/jats:italic>\n                    is widely expressed, with the highest levels found in the gill. Notably, the expression of\n                    <jats:italic>FADD<\/jats:italic>\n                    in peripheral blood, spleen, head kidney, and brain tissues changed significantly with the invasion of\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>S. agalactiae<\/jats:italic>\n                    <\/jats:styled-content>\n                    . Furthermore, ELISA and absorbance assays confirmed that FADD binds to\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>S. agalactiae<\/jats:italic>\n                    <\/jats:styled-content>\n                    and inhibits its growth, indicating the participation in tilapia resistance against\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>S. agalactiae<\/jats:italic>\n                    <\/jats:styled-content>\n                    infection.\n                  <\/jats:p>","DOI":"10.1111\/jfd.70217","type":"journal-article","created":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T01:25:01Z","timestamp":1779845101000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Identification, Molecular Characterisation, Expression and Functional Analysis of FADD in Response to\n                    <i>Streptococcus agalactiae<\/i>\n                    Infection in Nile Tilapia"],"prefix":"10.1111","volume":"49","author":[{"given":"Jingjing","family":"Ran","sequence":"first","affiliation":[{"name":"Hainan Institute of Northwest A&amp;F University  Sanya Hainan Province China"},{"name":"Guangxi Key Laboratory of Aquatic Biotechnology and Modern Ecological Aquaculture, Guangxi Academy of Marine Sciences Guangxi Academy of Sciences  Nanning Guangxi China"},{"name":"College of Animal Science and Technology Northwest A&amp;F University  Yangling Shaanxi China"},{"name":"Northwest A&amp;F University Shenzhen Research Institute  Shenzhen Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Du","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Aquatic Biotechnology and Modern Ecological Aquaculture, Guangxi Academy of Marine Sciences Guangxi Academy of Sciences  Nanning Guangxi China"},{"name":"Northwest A&amp;F University Shenzhen Research Institute  Shenzhen Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Longkun","family":"Gao","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Aquatic Biotechnology and Modern Ecological Aquaculture, Guangxi Academy of Marine Sciences Guangxi Academy of Sciences  Nanning Guangxi China"},{"name":"Northwest A&amp;F University Shenzhen Research Institute  Shenzhen Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuizheng","family":"Kong","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Aquatic Biotechnology and Modern Ecological Aquaculture, Guangxi Academy of Marine Sciences Guangxi Academy of Sciences  Nanning Guangxi China"},{"name":"Northwest A&amp;F University Shenzhen Research Institute  Shenzhen Guangdong China"},{"name":"College of Information Engineering Northwest A&amp;F University  Yangling Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erlei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Marine Natural Products and Combinatorial Biosynthesis Chemistry Guangxi Academy of Sciences  Nanning Guangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7124-7084","authenticated-orcid":false,"given":"Mingzhu","family":"Liu","sequence":"additional","affiliation":[{"name":"Hainan Institute of Northwest A&amp;F University  Sanya Hainan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Yu","sequence":"additional","affiliation":[{"name":"Hainan Institute of Northwest A&amp;F University  Sanya Hainan Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dengfeng","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology Northwest A&amp;F University  Yangling Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9465-108X","authenticated-orcid":false,"given":"Gaoxue","family":"Wang","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Aquatic Biotechnology and Modern Ecological Aquaculture, Guangxi Academy of Marine Sciences Guangxi Academy of Sciences  Nanning Guangxi China"},{"name":"Northwest A&amp;F University Shenzhen Research Institute  Shenzhen Guangdong China"},{"name":"College of Information Engineering Northwest A&amp;F University  Yangling Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5042-4093","authenticated-orcid":false,"given":"Tianqiang","family":"Liu","sequence":"additional","affiliation":[{"name":"Guangxi Key Laboratory of Aquatic Biotechnology and Modern Ecological Aquaculture, Guangxi Academy of Marine Sciences Guangxi Academy of Sciences  Nanning Guangxi 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A.Schmitt andG.Chiocchia.2009.\u201cIn Vivo Localization of Fas\u2010Associated Death Domain Protein in the Nucleus and Cytoplasm of Normal Thyroid and Liver Cells.\u201darXiv Preprint arXiv:0906.3936.","DOI":"10.2174\/1876894600901010027"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.12681"},{"key":"e_1_2_10_32_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M000811200"},{"key":"e_1_2_10_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2024.110110"},{"key":"e_1_2_10_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2022.06.046"},{"key":"e_1_2_10_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2025.110961"},{"key":"e_1_2_10_36_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.279.5358.1954"},{"key":"e_1_2_10_37_1","doi-asserted-by":"publisher","DOI":"10.14411\/eje.2022.003"},{"key":"e_1_2_10_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2018.01.018"},{"key":"e_1_2_10_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2019.04.030"}],"container-title":["Journal of Fish Diseases"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.70217","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jfd.70217","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jfd.70217","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T12:55:30Z","timestamp":1790859330000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jfd.70217"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,26]]},"references-count":38,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2026,11]]}},"alternative-id":["10.1111\/jfd.70217"],"URL":"https:\/\/doi.org\/10.1111\/jfd.70217","archive":["Portico"],"relation":{},"ISSN":["0140-7775","1365-2761"],"issn-type":[{"value":"0140-7775","type":"print"},{"value":"1365-2761","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,26]]},"assertion":[{"value":"2026-03-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-21","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-26","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70217"}}