{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T13:55:28Z","timestamp":1790862928403,"version":"4.1.0"},"reference-count":26,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T00:00:00Z","timestamp":1782172800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T00:00:00Z","timestamp":1782172800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2026,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Circadian rhythms regulate immune function in vertebrates; however, their contribution to infection outcomes in teleost fish remains unclear. Here, we examined whether infection timing influences host defence against\n                    <jats:italic>Edwardsiella piscicida<\/jats:italic>\n                    in Japanese medaka (\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Oryzias latipes<\/jats:italic>\n                    <\/jats:styled-content>\n                    ). Fish were challenged by immersion with\n                    <jats:italic>E. piscicida<\/jats:italic>\n                    at ZT2 or ZT14 under a 12\u2009h:12\u2009h light\u2013dark cycle, and survival was monitored for 16\u2009days. To evaluate early immune responses, the expression of Toll\u2010like receptors (TLRs) and pro\u2010inflammatory cytokines in head kidney was analysed 6\u2009h after infection by quantitative real\u2010time PCR. Mortality began on day 3 post\u2010infection, and survival differed significantly based on infection time: fish infected at ZT2 showed 75% survival, whereas those infected at ZT14 exhibited 25% survival. Expression levels of\n                    <jats:italic>tlr1<\/jats:italic>\n                    ,\n                    <jats:italic>tlr5m<\/jats:italic>\n                    ,\n                    <jats:italic>tlr21<\/jats:italic>\n                    ,\n                    <jats:italic>il1b<\/jats:italic>\n                    and\n                    <jats:italic>tnfa<\/jats:italic>\n                    were significantly higher in fish infected at ZT2 than in those infected at ZT14, while\n                    <jats:italic>tlr9<\/jats:italic>\n                    showed a similar, but non\u2010significant trend. These results indicate that early induction of pathogen recognition receptors and inflammatory cytokines in medaka differs according to the time of infection and is associated with different outcomes in host survival. Our findings suggest that time\u2010of\u2010day\u2010dependent factors are important determinants of\n                    <jats:italic>E. piscicida<\/jats:italic>\n                    infection outcomes in teleost fish.\n                  <\/jats:p>","DOI":"10.1111\/jfd.70226","type":"journal-article","created":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T09:15:21Z","timestamp":1782206121000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Time\u2010of\u2010Day\u2010Dependent Host Defence Against\n                    <i>Edwardsiella<\/i>\n                    Infection in Medaka (\n                    <i>Oryzias latipes<\/i>\n                    )"],"prefix":"10.1111","volume":"49","author":[{"given":"Takahiko","family":"Hata","sequence":"first","affiliation":[{"name":"Course of Marine Biological Sciences, Department of Applied Biological Science, Interdisciplinary Graduate School of Agriculture and Engineering University of Miyazaki  Miyazaki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miyu","family":"Hirayoshi","sequence":"additional","affiliation":[{"name":"Course of Biochemistry and Applied Biosciences, Graduate School of Agriculture University of Miyazaki  Miyazaki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiyori","family":"Iwai","sequence":"additional","affiliation":[{"name":"Course of Biochemistry and Applied Biosciences, Graduate School of Agriculture University of Miyazaki  Miyazaki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuiko","family":"Kuroyanagi","sequence":"additional","affiliation":[{"name":"Course of Biochemistry and Applied Biosciences, Graduate School of Agriculture University of Miyazaki  Miyazaki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minato","family":"Funakura","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Applied Biosciences Faculty of Agriculture, University of Miyazaki  Miyazaki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun\u2010ichi","family":"Hikima","sequence":"additional","affiliation":[{"name":"Unit of Marine Life Science, Department of Agriculture Faculty of Agriculture, University of Miyazaki  Miyazaki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4164-7594","authenticated-orcid":false,"given":"Tomoya","family":"Kono","sequence":"additional","affiliation":[{"name":"Unit of Marine Life Science, Department of Agriculture Faculty of Agriculture, University of Miyazaki  Miyazaki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,23]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008\u20105472.CAN\u201004\u20100496"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1120636110"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3354\/dao03281"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010017\u201012842\u20101"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature11088"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1601895113"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2024.105143"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1701677"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0906361106"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2017.11.025"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2005.12.005"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2023.108979"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0165799"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.3147\/jsfp.58.175"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1128\/genomeA.00546\u201014"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2019.02.004"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2020.00053"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1577\/h08\u2010061.1"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3386"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467\u2010019\u201011400\u20109"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2011.12.017"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2018.e00579"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2020.07.009"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2022.06.020"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13578\u2010022\u201000947\u20108"}],"container-title":["Journal 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