{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T13:54:21Z","timestamp":1790862861729,"version":"4.1.0"},"reference-count":16,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T00:00:00Z","timestamp":1782432000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T00:00:00Z","timestamp":1782432000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["88887.078833\/2024\u201000"],"award-info":[{"award-number":["88887.078833\/2024\u201000"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["Finance Code 001"],"award-info":[{"award-number":["Finance Code 001"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["312684\/2022\u20103"],"award-info":[{"award-number":["312684\/2022\u20103"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Fish Diseases"],"published-print":{"date-parts":[[2026,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Columnaris is an important bacterial disease of freshwater fish and is increasingly recognised as being caused by a species complex historically referred to as\n                    <jats:styled-content style=\"fixed-case\">\n                      <jats:italic>Flavobacterium columnare<\/jats:italic>\n                    <\/jats:styled-content>\n                    . A two\u2010step molecular workflow was applied to ornamental fish with clinical signs of columnaris disease from a multispecies commercial distributor operating a shared recirculating system in S\u00e3o Paulo, Brazil. Skin and fin lesion scrapings were Gram stained, and lesion\u2010derived tissue DNA was screened by ISR\u2010PCR for the columnaris disease complex. ISR\u2010PCR yielded the expected amplicon in 61\/123 samples (49.6%) from 28 ornamental fish species. All ISR\u2010PCR\u2010positive samples were then analysed by multiplex PCR for species assignment. Group\u2010specific amplicons were obtained from 29\/61 samples (47.5%):\n                    <jats:italic>Flavobacterium covae<\/jats:italic>\n                    in 27\/61 (44.3%) and\n                    <jats:italic>F. davisii<\/jats:italic>\n                    in 2\/61 (3.3%); no GG1 or GG4 amplicons were detected. The remaining 32\/61 samples (52.5%) were not typeable by multiplex PCR. These findings provide baseline species\u2010level data and support combined molecular surveillance in multispecies ornamental fish systems.\n                  <\/jats:p>","DOI":"10.1111\/jfd.70228","type":"journal-article","created":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T04:30:12Z","timestamp":1782534612000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Two\u2010Step PCR Workflow Reveals Predominance of\n                    <i>Flavobacterium covae<\/i>\n                    in Ornamental Fish With Clinical Signs of Columnaris Disease"],"prefix":"10.1111","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8387-0121","authenticated-orcid":false,"given":"Pedro Henrique Magalh\u00e3es","family":"Cardoso","sequence":"first","affiliation":[{"name":"Department of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine and Animal Science University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emy","family":"Yano","sequence":"additional","affiliation":[{"name":"Department of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine and Animal Science University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simone","family":"de Carvalho Balian","sequence":"additional","affiliation":[{"name":"Department of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine and Animal Science University of S\u00e3o Paulo  S\u00e3o Paulo Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andr\u00e9 Pegoraro","family":"Poor","sequence":"additional","affiliation":[{"name":"Department of Preventive Veterinary Medicine and Animal Health, School of Veterinary Medicine and Animal Science University of S\u00e3o Paulo  S\u00e3o Paulo 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