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Alternative control strategies for this pathogen remain limited. In this study, two lytic jumbo bacteriophages, DJC6219 and NK62157, were isolated from municipal wastewater and characterized for their potential as candidate biocontrol agents against\n                    <jats:italic>A. dhakensis<\/jats:italic>\n                    . Both phages were evaluated using host range assays, transmission electron microscopy, stability tests, multiplicity of infection (MOI) optimization, growth kinetics, and genomic analyses. Both phages showed high specificity toward\n                    <jats:italic>A. dhakensis<\/jats:italic>\n                    . They had icosahedral heads and long non\u2010contractile tails, consistent with siphovirus\u2010like phages. Both phages remained stable within moderate temperature and pH ranges but showed reduced viability under extreme conditions. Growth inhibition was observed at MOIs of 0.1\u20131, while a phage cocktail achieved enhanced bacterial suppression at an MOI of 0.01. Genomic analysis confirmed that both phages are lytic and carried no antimicrobial resistance genes, virulence factors, or lysogeny\u2010related genes. Comparative analysis indicated that both phages are closely related to known\n                    <jats:italic>Aeromonas<\/jats:italic>\n                    phages while representing independent environmental isolates. Our findings highlight the potential of DJC6219 and NK62157 as candidate biocontrol agents against\n                    <jats:italic>A. dhakensis<\/jats:italic>\n                    in aquaculture.\n                  <\/jats:p>","DOI":"10.1111\/jfd.70235","type":"journal-article","created":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T05:07:48Z","timestamp":1783400868000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Isolation and In\u00a0Vitro Characterization of Two Lytic Jumbo Bacteriophages Targeting\n                    <i>Aeromonas dhakensis<\/i>\n                    for Biocontrol in Aquaculture"],"prefix":"10.1111","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6614-5275","authenticated-orcid":false,"given":"Tharindu Polwatta","family":"Gallage","sequence":"first","affiliation":[{"name":"Program in Applied Biological Sciences Chulabhorn Graduate Institute  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phongsawat","family":"Paisantham","sequence":"additional","affiliation":[{"name":"Research Laboratory of Biotechnology Chulabhorn Research Institute  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thitima","family":"Srathongneam","sequence":"additional","affiliation":[{"name":"Research Laboratory of Biotechnology Chulabhorn Research Institute  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heera","family":"Rajandas","sequence":"additional","affiliation":[{"name":"Centre of Excellence for Omics\u2010Driven Computational Biodiscovery (COMBio) AIMST University  Bedong Kedah Malaysia"},{"name":"Center for Evolutionary Hologenomics, Globe Institute University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sivachandran","family":"Parimannan","sequence":"additional","affiliation":[{"name":"Centre of Excellence for Omics\u2010Driven Computational Biodiscovery (COMBio) AIMST University  Bedong Kedah Malaysia"},{"name":"Center for Evolutionary Hologenomics, Globe Institute University of Copenhagen  Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eakapol","family":"Wangkahart","sequence":"additional","affiliation":[{"name":"Laboratory of Fish Immunology and Nutrigenomics, Applied Animal and Aquatic Sciences Research Unit, Faculty of Technology Mahasarakham University  Kantarawichai Mahasarakham Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Win","family":"Surachetpong","sequence":"additional","affiliation":[{"name":"Institute of Aquaculture University of Stirling  Stirling UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rojana","family":"Sukchawalit","sequence":"additional","affiliation":[{"name":"Program in Applied Biological Sciences Chulabhorn Graduate Institute  Bangkok Thailand"},{"name":"Research Laboratory of Biotechnology Chulabhorn Research Institute  Bangkok Thailand"},{"name":"Center of Excellence on Environmental Health and Toxicology (EHT), OPS, MHESI  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Skorn","family":"Mongkolsuk","sequence":"additional","affiliation":[{"name":"Research Laboratory of Biotechnology Chulabhorn Research Institute  Bangkok Thailand"},{"name":"Center of Excellence on Environmental Health and Toxicology (EHT), OPS, MHESI  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7273-4060","authenticated-orcid":false,"given":"Kwanrawee","family":"Sirikanchana","sequence":"additional","affiliation":[{"name":"Research Laboratory of Biotechnology Chulabhorn Research Institute  Bangkok Thailand"},{"name":"Center of Excellence on Environmental Health and Toxicology (EHT), OPS, MHESI  Bangkok Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,6]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/jfd.13164"},{"issue":"4","key":"e_1_2_11_3_1","doi-asserted-by":"crossref","DOI":"10.3390\/antibiotics12040723","article-title":"Bacteriophage Adsorption: Likelihood of Virion Encounter With Bacteria and Other Factors Affecting Rates","volume":"12","author":"Abedon S. 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